Variation in the fluorescence decay properties of haematoporphyrin derivative during its conversion to photoproducts.

Haematoporphyrin derivative (HpD) photoproducts are formed in aqueous solutions during light exposure in the presence of oxygen. The evaluation of the fluorescence decay of the photoproduct-enriched HpD solution shows an increase in the short-lived components, especially about 2 ns, in comparison with HpD without photoproducts. The bleaching of the HpD fluorescence and the photoproduct formation by the fluorescence-exciting radiation has to be taken into account in the evaluation of stationary as well as time-resolved fluorescence measurements.

[1]  A. McLean,et al.  Detection of haematoporphyrin derivative and haematoporphyrin excited states in cell environments. , 1988, Cancer letters.

[2]  G. Jonusauskas,et al.  Light-induced transformations of hematoporphyrin diacetate and hematoporphyrin. , 1988, Journal of photochemistry and photobiology. B, Biology.

[3]  Rinaldo Cubeddu,et al.  Hematoporphyrin derivative: experimental evidence for aggregated species , 1982 .

[4]  R. Santus,et al.  SELECTIVE TYPE I PHOTOOXIDATIONS IN MIXTURES OF PORPHYRINS AND ELECTROPHILIC NITROIMIDAZOLES , 1986 .

[5]  J Moan,et al.  Photoproducts formed from photofrin II in cells. , 1988, Journal of photochemistry and photobiology. B, Biology.

[6]  T. Seki,et al.  Fluorescence of hematoporphyrin in living cells and in solution. , 1988, Journal of photochemistry and photobiology. B, Biology.

[7]  H. Schneckenburger,et al.  Time-resolved fluorescence in photobiology. , 1988, Journal of photochemistry and photobiology. B, Biology.