In vivo nuclear magnetic resonance study of citrate metabolism in Propionibacterium freudenreichii subsp. shermanii

Citrate metabolism by resting cells of Propionibacterium freudenreichii subsp. shermanii was investigated. In vivo13C nuclear magnetic resonance spectroscopy was used to study the pathway of citrate breakdown and to probe its utilization, non-invasively, in living cell suspensions. [2,4-13C]citrate was metabolized by resting cells to glutamate labelled in positions 2 and 4. In the presence of lactate or pyruvate, its rate of consumption was faster, but it was still converted to glutamate. No catabolic pathway other than the first third of a turn of the tricarboxylic acid cycle was used by Prop. freudenreichii subsp. shermanii to degrade citrate.

[1]  W. Grosch,et al.  Evaluation of taste compounds of Swiss cheese (Emmentaler) , 1996 .

[2]  H Sahm,et al.  Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing , 1996, Biotechnology and bioengineering.

[3]  P. Boyaval,et al.  Proline-specific activities of Propionibacterium freudenreichii subsp. shermanii , 1995, Journal of Dairy Research.

[4]  H. Santos,et al.  Enzyme Basis for pH Regulation of Citrate and Pyruvate Metabolism by Leuconostoc oenos , 1995, Applied and environmental microbiology.

[5]  G. Bécard,et al.  In vivo nuclear magnetic resonance study of the osmoregulation of phosphocholine-substituted beta-1,3;1,6 cyclic glucan and its associated carbon metabolism in Bradyrhizobium japonicum USDA 110 , 1994, Applied and environmental microbiology.

[6]  A. Ramos,et al.  13C Nuclear Magnetic Resonance Studies of Citrate and Glucose Cometabolism by Lactococcus lactis , 1994, Applied and environmental microbiology.

[7]  W. Verhue,et al.  Study of the Citrate Metabolism of Lactococcus lactis subsp. lactis Biovar Diacetylactis by Means of 13C Nuclear Magnetic Resonance , 1991, Applied and environmental microbiology.

[8]  P. Schmitt,et al.  Co-metabolism of citrate and lactose by Leuconostoc mesenteroides subsp. cremoris , 1991 .

[9]  T. Miller,et al.  Acetate production from hydrogen and [13C]carbon dioxide by the microflora of human feces , 1988, Applied and environmental microbiology.

[10]  A. J. Shaka,et al.  Computer-optimized decoupling scheme for wideband applications and low-level operation , 1985 .

[11]  J. Zeikus,et al.  Single-carbon catabolism in acetogens: analysis of carbon flow in Acetobacterium woodii and Butyribacterium methylotrophicum by fermentation and 13C nuclear magnetic resonance measurement , 1983, Journal of bacteriology.

[12]  A. J. Shaka,et al.  An improved sequence for broadband decoupling: WALTZ-16 , 1983 .

[13]  T. Langsrud,et al.  FLAVOR DEVELOPMENT AND MICROBIOLOGY OF SWISS CHEESE–A REVIEW: II. STARTERS, MANUFACTURING PROCESSES AND PROCEDURES , 1973 .

[14]  E. Vedamuthu,et al.  An evaluation of the taxonomy of Propionibacterium. , 1968, Canadian journal of microbiology.