The detection of renal carcinoma cells in the peripheral blood with an enhanced reverse transcriptase–polymerase chain reaction assay for MN/CA9

Using a reverse transcriptase–polymerase chain reaction (RT‐PCR) assay, the authors previously determined the expression of MN/CA9 mRNA in renal cell carcinoma (RCC) and its absence in benign renal tissue. In the current study, the utility of an enhanced RT‐PCR assay in the detection of renal carcinoma cells in the peripheral blood was assessed.

[1]  P. Elson Prognostic Factors in Metastatic Renal Cell Carcinoma , 2000 .

[2]  P. Pisters Chemoradiation treatment strategies for localized sarcoma: conventional and investigational approaches. , 1999, Seminars in surgical oncology.

[3]  Taylor Murray,et al.  Cancer statistics, 1998 , 1998, CA: a cancer journal for clinicians.

[4]  E. Stanbridge,et al.  Identification of the MN/CA9 protein as a reliable diagnostic biomarker of clear cell carcinoma of the kidney. , 1997, Cancer research.

[5]  M. Stifelman,et al.  Expression of the tumor-associated gene MN: a potential biomarker for human renal cell carcinoma. , 1997, Cancer research.

[6]  J. Pastorek,et al.  Transient transformation of mammalian cells by MN protein, a tumor-associated cell adhesion molecule with carbonic anhydrase activity. , 1997, International journal of oncology.

[7]  K. Junker,et al.  Transforming growth factor beta 1 is significantly elevated in plasma of patients suffering from renal cell carcinoma. , 1998, Cytokine.

[8]  G. Snow,et al.  Squamous cell carcinoma-associated antigens used in novel strategies for the detection and treatment of minimal residual head and neck cancer. , 1996, Anticancer research.

[9]  R. Kettmann,et al.  Human MN/CA9 gene, a novel member of the carbonic anhydrase family: structure and exon to protein domain relationships. , 1996, Genomics.

[10]  E. Levine,et al.  Detection of tyrosinase mRNA from the blood of melanoma patients. , 1996, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[11]  F. Marshall,et al.  Serum ferritin as a clinical marker for renal cell carcinoma: influence of tumor volume. , 1995, Urology.

[12]  K. Pittman,et al.  Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. , 1995, British Journal of Cancer.

[13]  R. Kettmann,et al.  Cloning and characterization of MN, a human tumor-associated protein with a domain homologous to carbonic anhydrase and a putative helix-loop-helix DNA binding segment. , 1994, Oncogene.

[14]  P. Disaia,et al.  Identification of the MN antigen as a diagnostic biomarker of cervical intraepithelial squamous and glandular neoplasia and cervical carcinomas. , 1994, The American journal of pathology.

[15]  M. Benson,et al.  Molecular staging of prostate cancer with the use of an enhanced reverse transcriptase-PCR assay. , 1994, Urology.

[16]  E. Dmitrovsky,et al.  Detection of minimal residual disease in acute promyelocytic leukemia by a reverse transcription polymerase chain reaction assay for the PML/RAR-alpha fusion mRNA. , 1993, Blood.

[17]  A. Gross,et al.  Neuron-specific enolase: a serum tumor marker in renal cell carcinoma? , 1993, European urology.

[18]  S. Pastoreková,et al.  A novel quasi-viral agent, MaTu, is a two-component system. , 1992, Virology.

[19]  K. Grankvist,et al.  Erythropoietin in renal cell carcinoma: evaluation of its usefulness as a tumor marker. , 1992, European urology.

[20]  I. Moro,et al.  Tumor markers in human renal cell carcinoma. , 1991, Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine.

[21]  M. Melamed,et al.  Analysis of a mouse monoclonal antibody that reacts with a specific region of the human proximal tubule and subsets renal cell carcinomas. , 1989, Cancer research.

[22]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[23]  J. Dekernion,et al.  Prognostic factors in metastatic renal carcinoma. , 1986, The Journal of urology.

[24]  K. Mullis,et al.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. , 1985, Science.

[25]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .