Method of applying sanitizers and sample preparation affects recovery of native microflora and Salmonella on whole cantaloupe surfaces.

Standardized methods for applying sanitizer treatments to cantaloupes and for recovering surviving native microflora or Salmonella on inoculated cantaloupe after sanitizing are lacking. Accordingly, the objectives of this study were to compare four methods for applying sanitizers (dipping, dipping with rotation, dipping with agitation, and dipping with rubbing) using 200 ppm of chlorine or 5% H2O2, two recovery methods (homogenization of rind plugs in a stomacher or blender), and five selective recovery media for Salmonella. Whole cantaloupes were submerged in a cocktail of five strains of Salmonella (each at approximately 2 x 10(8) CFU/ml) for 10 min and allowed to dry for 1 h inside a biosafety cabinet and stored at 20 degrees C for approximately 23 h before sanitizing. The recovery of Salmonella from whole cantaloupe without sanitizing averaged 5.09 log CFU/cm2 by blending and 4.30 log CFU/cm2 by homogenization in a stomacher for the five selective agar media. Microbial populations (Salmonella or the indigenous aerobic mesophilic bacteria, gram-negative bacteria, lactic acid bacteria, Pseudomonas spp., and yeast and mold) were not significantly (P > 0.05) reduced by treating with water regardless of the treatment method used. Sanitizing with chlorine or H2O2 by dipping, with or without rotation for 2 min, also did not reduce microbial populations. However, populations of all classes of native microflora and Salmonella were significantly (P < 0.05) reduced by sanitizer treatments (2 min) applied with agitation or by rubbing. In general, sanitizer treatments applied by rubbing resulted in greater log reductions (by up to 1.7 log unit) than for treatments applied with agitation. Populations of native microflora and Salmonella recovered from cantaloupe were higher (by up to 1.8 log unit) by blending compared to homogenization in a stomacher. In most instances, selective media used did not differ significantly (P > 0.05) for recovery of Salmonella after washing treatments.

[1]  J. Wells,et al.  Factors influencing the detection and enumeration of Escherichia coli O157:H7 on alfalfa seeds. , 2001, International journal of food microbiology.

[2]  C. M. Park,et al.  Evaluation of sanitizers for killing Escherichia coli O157: H7, Salmonella, and naturally occurring microorganisms on cantaloupes, honeydew melons, and asparagus , 1999 .

[3]  M. Tamplin.  SALMONELLA AND CANTALOUPES , 1997 .

[4]  M. Osterholm,et al.  Multistate outbreak of Salmonella poona infections - United States and Canada, 1991 , 1991 .

[5]  William F. Fett,et al.  Effectiveness of chlorine and nisin-edta treatments of whole melons and fresh-cut pieces for reducing native microflora and extending shelf-life , 2002 .

[6]  C. Hedberg,et al.  Food-related illness and death in the United States. , 1999, Emerging infectious diseases.

[7]  William C Hurst,et al.  Fresh Produce Processing -- an Industry Perspective. , 1992, Journal of food protection.

[8]  E. H. Garrett,et al.  Standardization of a method to determine the efficacy of sanitizers in inactivating human pathogenic microorganisms on raw fruits and vegetables. , 2001, Journal of food protection.

[9]  G. M. Sapers,et al.  Effect of sanitizer treatments on Salmonella Stanley attached to the surface of cantaloupe and cell transfer to fresh-cut tissues during cutting practices. , 2001, Journal of food protection.

[10]  G. M. Sapers,et al.  INFLUENCE OF WASHING TREATMENT ON NATIVE MICROFLORA AND ESCHERICHIA COLI POPULATION OF INOCULATED CANTALOUPES , 2001 .

[11]  L. Harris,et al.  Efficacy and reproducibility of a produce wash in killing Salmonella on the surface of tomatoes assessed with a proposed standard method for produce sanitizers. , 2001, Journal of food protection.

[12]  Larry R. Beuchat,et al.  Pathogenic Microorganisms Associated with Fresh Produce. , 1996, Journal of food protection.

[13]  Multistate outbreaks of Salmonella serotype Poona infections associated with eating cantaloupe from Mexico--United States and Canada, 2000-2002. , 2002, MMWR. Morbidity and mortality weekly report.

[14]  L. Beuchat,et al.  Comparison of sample preparation methods for recovering Salmonella from raw fruits, vegetables, and herbs. , 2001, Journal of food protection.