FRET enhancement close to gold nanoparticles positioned in DNA origami constructs.

Here we investigate the energy transfer rates of a Förster resonance energy transfer (FRET) pair positioned in close proximity to a 5 nm gold nanoparticle (AuNP) on a DNA origami construct. We study the distance dependence of the FRET rate by varying the location of the donor molecule, D, relative to the AuNP while maintaining a fixed location of the acceptor molecule, A. The presence of the AuNP induces an alteration in the spontaneous emission of the donor (including radiative and non-radiative rates) which is strongly dependent on the distance between the donor and AuNP surface. Simultaneously, the energy transfer rates are enhanced at shorter D-A (and D-AuNP) distances. Overall, in addition to the direct influence of the acceptor and AuNP on the donor decay there is also a significant increase in decay rate not explained by the sum of the two interactions. This leads to enhanced energy transfer between donor and acceptor in the presence of a 5 nm AuNP. We also demonstrate that the transfer rate in the three "particle" geometry (D + A + AuNP) depends approximately linearly on the transfer rate in the donor-AuNP system, suggesting the possibility to control FRET process with electric field induced by 5 nm AuNPs close to the donor fluorophore. It is concluded that DNA origami is a very versatile platform for studying interactions between molecules and plasmonic nanoparticles in general and FRET enhancement in particular.

[1]  H. Rigneault,et al.  Matching Nanoantenna Field Confinement to FRET Distances Enhances Förster Energy Transfer Rates. , 2015, Nano letters (Print).

[2]  M. Metzger,et al.  Controlling the dynamics of Förster resonance energy transfer inside a tunable sub-wavelength Fabry-Pérot-resonator. , 2015, Nanoscale.

[3]  Qinghua Xu,et al.  Single-Particle Spectroscopic Study on Fluorescence Enhancement by Plasmon Coupled Gold Nanorod Dimers Assembled on DNA Origami. , 2015, The journal of physical chemistry letters.

[4]  J. Wenger,et al.  FRET enhancement in aluminum zero-mode waveguides. , 2015, Chemphyschem : a European journal of chemical physics and physical chemistry.

[5]  Christof M Niemeyer,et al.  Reversible reconfiguration of DNA origami nanochambers monitored by single-molecule FRET. , 2015, Angewandte Chemie.

[6]  Energy transfer in the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, analyzed by time-resolved fluorescence spectroscopies , 2015, Photosynthesis Research.

[7]  Hao Yan,et al.  Fluorescence quenching of quantum dots by gold nanoparticles: a potential long range spectroscopic ruler. , 2014, Nano letters.

[8]  Hao Yan,et al.  Hierarchical assembly of plasmonic nanostructures using virus capsid scaffolds on DNA origami templates. , 2014, ACS nano.

[9]  F. Schleifenbaum,et al.  Dynamic control of Förster energy transfer in a photonic environment. , 2014, Physical chemistry chemical physics : PCCP.

[10]  Paramjit S. Arora,et al.  Amyloid fibrils nucleated and organized by DNA origami constructions , 2014, Nature nanotechnology.

[11]  Tim Liedl,et al.  Plasmonic DNA-origami nanoantennas for surface-enhanced Raman spectroscopy. , 2014, Nano letters.

[12]  Weihai Ni,et al.  DNA origami-directed, discrete three-dimensional plasmonic tetrahedron nanoarchitectures with tailored optical chirality. , 2014, ACS applied materials & interfaces.

[13]  Philip Tinnefeld,et al.  Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids. , 2014, Nano letters.

[14]  Tao Zhang,et al.  DNA origami based assembly of gold nanoparticle dimers for surface-enhanced Raman scattering , 2014, Nature Communications.

[15]  J. Wenger,et al.  Nanophotonic enhancement of the Förster resonance energy-transfer rate with single nanoapertures. , 2014, Nano letters.

[16]  Vladimir Lesnyak,et al.  Experimental and theoretical investigation of the distance dependence of localized surface plasmon coupled Förster resonance energy transfer. , 2014, ACS nano.

[17]  M. Bathe,et al.  Structure-based model for light-harvesting properties of nucleic acid nanostructures , 2013, Nucleic acids research.

[18]  J. R. Zurita-Sánchez,et al.  A revisitation of the Förster energy transfer near a metallic spherical nanoparticle: (1) Efficiency enhancement or reduction? (2) The control of the Förster radius of the unbounded medium. (3) The impact of the local density of states. , 2013, The Journal of chemical physics.

[19]  Weihai Ni,et al.  Bifacial DNA origami-directed discrete, three-dimensional, anisotropic plasmonic nanoarchitectures with tailored optical chirality. , 2013, Journal of the American Chemical Society.

[20]  B. Albinsson,et al.  Photon upconversion facilitated molecular solar energy storage , 2013 .

[21]  Hao Yan,et al.  Quantum Efficiency Modification of Organic Fluorophores Using Gold Nanoparticles on DNA Origami Scaffolds , 2013 .

[22]  M. Francis,et al.  Controlled integration of gold nanoparticles and organic fluorophores using synthetically modified MS2 viral capsids. , 2013, Journal of the American Chemical Society.

[23]  B. Albinsson,et al.  Self-assembled nanoscale DNA-porphyrin complex for artificial light harvesting. , 2013, Journal of the American Chemical Society.

[24]  J Alexander Liddle,et al.  Quantum-dot fluorescence lifetime engineering with DNA origami constructs. , 2013, Angewandte Chemie.

[25]  A. L. Bradley,et al.  Effect of Metal Nanoparticle Concentration on Localized Surface Plasmon Mediated Förster Resonant Energy Transfer , 2012 .

[26]  Philip Tinnefeld,et al.  Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas , 2012, Science.

[27]  Jinkyu Lee,et al.  Switching off FRET in the hybrid assemblies of diblock copolymer micelles, quantum dots, and dyes by plasmonic nanoparticles. , 2012, ACS nano.

[28]  Tim Liedl,et al.  Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami. , 2012, ACS nano.

[29]  Tian Ming,et al.  Plasmon-Controlled Förster Resonance Energy Transfer , 2012 .

[30]  Baoquan Ding,et al.  Rolling up gold nanoparticle-dressed DNA origami into three-dimensional plasmonic chiral nanostructures. , 2012, Journal of the American Chemical Society.

[31]  A. Mosk,et al.  Nanophotonic control of the Förster resonance energy transfer efficiency. , 2011, Physical review letters.

[32]  F. Simmel,et al.  DNA-based self-assembly of chiral plasmonic nanostructures with tailored optical response , 2011, Nature.

[33]  Vladimir Lesnyak,et al.  Surface plasmon enhanced energy transfer between donor and acceptor CdTe nanocrystal quantum dot monolayers. , 2011, Nano letters.

[34]  Tim Liedl,et al.  Single-molecule FRET ruler based on rigid DNA origami blocks. , 2011, Chemphyschem : a European journal of chemical physics and physical chemistry.

[35]  Hao Yan,et al.  DNA-origami-directed self-assembly of discrete silver-nanoparticle architectures. , 2010, Angewandte Chemie.

[36]  Hao Yan,et al.  Gold nanoparticle self-similar chain structure organized by DNA origami. , 2010, Journal of the American Chemical Society.

[37]  Friedrich C. Simmel,et al.  DNA Origami as a Nanoscopic Ruler for Super‐Resolution Microscopy , 2009 .

[38]  T. Brown,et al.  Nucleic acid base analog FRET-pair facilitating detailed structural measurements in nucleic acid containing systems. , 2009, Journal of the American Chemical Society.

[39]  B. Nordén,et al.  Membrane-anchored DNA assembly for energy and electron transfer. , 2009, Journal of the American Chemical Society.

[40]  G. Strouse,et al.  Tracking spatial disorder in an optical ruler by time-resolved NSET. , 2009, The journal of physical chemistry. B.

[41]  B. Albinsson,et al.  Self-assembled DNA photonic wire for long-range energy transfer. , 2008, Journal of the American Chemical Society.

[42]  Energy transfer between fluorescent dyes in photonic crystals. , 2008, Optics letters.

[43]  Conformational Flexibility in DNA Nanoconstructs: A Time-Resolved Fluorescence Resonance Energy Transfer Study , 2008 .

[44]  D. Lilley,et al.  The structure of cyanine 5 terminally attached to double-stranded DNA: implications for FRET studies. , 2008, Biochemistry.

[45]  Rahul Roy,et al.  A practical guide to single-molecule FRET , 2008, Nature Methods.

[46]  Jian Zhang,et al.  Enhanced Förster Resonance Energy Transfer on Single Metal Particle. 2. Dependence on Donor-Acceptor Separation Distance, Particle Size, and Distance from Metal Surface. , 2007, The journal of physical chemistry. C, Nanomaterials and interfaces.

[47]  J. Lakowicz,et al.  Enhanced Förster Resonance Energy Transfer (FRET) on Single Metal Particle. , 2007, The journal of physical chemistry. C, Nanomaterials and interfaces.

[48]  N. Kotov,et al.  Theory of plasmon-enhanced Förster energy transfer in optically excited semiconductor and metal nanoparticles , 2006, cond-mat/0612274.

[49]  M. Singh,et al.  Fluorescent lifetime quenching near d = 1.5 nm gold nanoparticles: probing NSET validity. , 2006, Journal of the American Chemical Society.

[50]  P. Rothemund Folding DNA to create nanoscale shapes and patterns , 2006, Nature.

[51]  T. Klar,et al.  Gold nanoparticles quench fluorescence by phase induced radiative rate suppression. , 2005, Nano letters.

[52]  N O Reich,et al.  Nanometal surface energy transfer in optical rulers, breaking the FRET barrier. , 2005, Journal of the American Chemical Society.

[53]  W. Barnes,et al.  Energy Transfer Across a Metal Film Mediated by Surface Plasmon Polaritons , 2004, Science.

[54]  Jean-Jacques Greffet,et al.  Single-molecule spontaneous emission close to absorbing nanostructures , 2004 .

[55]  Igor L. Medintz,et al.  Self-assembled nanoscale biosensors based on quantum dot FRET donors , 2003, Nature materials.

[56]  W. Eaton,et al.  Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy , 2002, Nature.

[57]  D. Ginger,et al.  Enhanced Förster energy transfer in organic/inorganic bilayer optical microcavities , 2001 .

[58]  Y. Kamagata,et al.  Fluorescence-Quenching Phenomenon by Photoinduced Electron Transfer between a Fluorescent Dye and a Nucleotide Base , 2001, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[59]  W. Barnes,et al.  Förster energy transfer in an optical microcavity. , 2000, Science.

[60]  S. Weiss Fluorescence spectroscopy of single biomolecules. , 1999, Science.

[61]  Markus Sauer,et al.  NUCLEOBASE-SPECIFIC QUENCHING OF FLUORESCENT DYES. 1. NUCLEOBASE ONE-ELECTRON REDOX POTENTIALS AND THEIR CORRELATION WITH STATIC AND DYNAMIC QUENCHING EFFICIENCIES , 1996 .

[62]  Mikael Kubista,et al.  Experimental correction for the inner-filter effect in fluorescence spectra , 1994 .

[63]  D. Millar,et al.  Distance distribution in a dye-linked oligonucleotide determined by time-resolved fluorescence energy transfer. , 1992, Biophysical chemistry.

[64]  Abraham Nitzan,et al.  Theory of energy transfer between molecules near solid state particles , 1985 .

[65]  A. Nitzan,et al.  Accelerated energy transfer between molecules near a solid particle , 1984 .

[66]  R. Ruppin,et al.  Decay of an excited molecule near a small metal sphere , 1982 .

[67]  K. Drexhage Influence of a dielectric interface on fluorescence decay time , 1970 .

[68]  L. Stryer,et al.  Energy transfer: a spectroscopic ruler. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[69]  E. Purcell,et al.  Resonance Absorption by Nuclear Magnetic Moments in a Solid , 1946 .