The attenuation of microwave emissions through the canopy is quantified by the vegetation optical depth (VOD), which is related to the amount of water, the biomass and the structure of vegetation. To provide microwave-derived plant water estimates, one must account for biomass/structure contributions in order to extract the water component from the VOD. This study uses Aquarius scatterometer data to build an L-band global seasonality of vegetation volume fraction (δ), representative of biomass/structure dynamics. The dynamic range of δ is adapted for its application in a gravimetric moisture (Mg) retrieval model. Results show that δ ranging from 0 to 3.35.10-4 is needed for modelling physically reasonable Mg values. The global average of δ shows consistent spatial patterns across vegetation distributions, and δ seasonality is coherent with the phenology of the studied vegetation types. These findings enable the separation of information on vegetation water and biomass/structure inherent within VOD.