OCCURRENCE OF AFLATOXIN M1 IN RAW MILK DURING THE SUMMER AND WINTER SEASONS IN HAMEDAN, IRAN

A survey on the occurrence of aflatoxin M 1 (AFM 1 ) was carried out in summer and winter in raw milk samples from 93 traditional and industrial dairy farms of the Hamedan district in order to address representative data on AFM 1 in milk collected from these regions. Of the 186 milk samples, AFM 1 was detected in 119 samples (63.97%). The mean concentration of AFM 1 in contaminated samples was 43.4 ng/L, and the minimum and maximum levels were ≤10 and 410 ng/L, respectively. Fourteen (11.76%) contaminated samples had AFM 1 in concentrations in excess of the maximum levels specified in European Union regulations, i.e., 50 ng/L. The contamination ratio of milk in summer and winter months was 56.5 and 71.7%, respectively (P < 0.02). This is the first report on AFM 1 determinations in raw milk from western Iran, one of the main milk-production areas, as well as comparison between milk obtained from different dairy farms in Iran.

[1]  J. Frémy,et al.  Recherche d'aflatoxine M1 dans les poudres de lactosérum. Evaluation saisonnière de la contamination , 1980 .

[2]  F. Chu Mycotoxins: food contamination, mechanism, carcinogenic potential and preventive measures. , 1991, Mutation research.

[3]  G. Barbieri,et al.  Aflatoxin M1 in Parmesan cheese: HPLC determination , 1994 .

[4]  A. Saad,et al.  Exposure of infants to aflatoxin M1 from mothers' breast milk in Abu Dhabi, UAE. , 1995, Food additives and contaminants.

[5]  H. P. Egmond Mycotoxins: Regulations, quality assurance and reference materials , 1995 .

[6]  M. Trucksess Committee on Natural Toxins:Mycotoxins , 1996 .

[7]  P. Markaki,et al.  Occurrence of aflatoxin M1 in commercial pasteurized milk determined with ELISA and HPLC. , 1997, Food additives and contaminants.

[8]  K. Saitanu Incidence of Aflatoxin M1 in Thai Milk Products. , 1997, Journal of food protection.

[9]  A. Biancardi Determinazione di aflatossina M1 nel latte : Metodologie immunochimiche e cromatografiche a confronto , 1997 .

[10]  A. Biancardi Determination of aflatoxin M1 residues in milk. A comparative assessment of ELISA and IAC-HPLC methods , 1997 .

[11]  C. A. Pinto,et al.  Immunochemical assessment of aflatoxin M1 in milk powder consumed by infants in São Paulo, Brazil. , 1997, Food additives and contaminants.

[12]  P. Bertuzzi,et al.  Aflatoxin M1 occurrence in samples of Grana Padano cheese. , 1997, Food additives and contaminants.

[13]  J. W. Park,et al.  Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC , 2000, Food additives and contaminants.

[14]  M. Quilliam,et al.  Committee on Natural Toxins: Mycotoxins: Phycotoxins: Plant Toxins , 2000 .

[15]  E. Panariti Seasonal variations of aflatoxin M1 in the farm milk in Albania. , 2001, Arhiv za higijenu rada i toksikologiju.

[16]  Joint Fao,et al.  Evaluation of certain mycotoxins in food. Fifty-sixth report of the Joint FAO/WHO Expert Committee on Food Additives. , 2002, World Health Organization technical report series.

[17]  L. Bulacio,et al.  Presence of aflatoxin M1 in milk for human consumption in Argentina , 2003 .

[18]  M. Trucksess Committee on natural toxins and food allergens. Mycotoxins. , 2003, Journal of AOAC International.

[19]  H. Akiyama,et al.  Occurrence of aflatoxin M1 in domestic milk in Japan during the winter season , 2004, Food additives and contaminants.

[20]  Premendra D. Dwivedi,et al.  Detection of Aflatoxin M1 contamination in milk and infant milk products from Indian markets by ELISA , 2004 .

[21]  M. A. Jonker,et al.  Worldwide regulations for mycotoxins in food and feed in 2003 , 2004 .

[22]  A. Kamkar,et al.  A study on the occurrence of aflatoxin M1 in raw milk produced in Sarab city of Iran , 2005 .

[23]  Behrooz Astaneh,et al.  Aflatoxin M1 contamination in pasteurized milk in Shiraz (south of Iran) , 2006 .

[24]  A. Kamkar A study on the occurrence of aflatoxin M1 in Iranian Feta cheese , 2006 .