Low-intensity laser therapy and led (light emitting diode) therapy in mechanical resistance of Rattus norvegicus chest inscision with implant of steel wire for sternal suture

INTRODUCTION: Low-intensity laser (LILT) and LED therapy are indicated in repairing surgical incisions, which occur in cardiac surgery. One major concern in this case is the presence of metallic wire used for sternal sutures, its temperature may rise while using a laser or LED. This study aimed to analyze the tensiometric properties of the skin tissue with longitudinal incision and metallic wire implant used for sternal suture irradiated with LILT and LED. METHODS: Twenty-five subjects (Rattus norvegicus) were submitted to thoracic incision with implantation of metallic wire, and subsequently divided into five groups: GI (control group); GII (laser group 6J/cm2); GIII (laser group 10J/cm2); GIV (LED group 6 J/cm2); and GV (LED 10J/cm2). Photobiomodulation was performed on a daily basis for eight consecutive days and the local temperature was measured before and after phototherapy. After euthanasia the tissues were subjected to tensiometric and histological analysis. RESULTS: All irradiated groups showed increase in break strength (p = 0.009), and decrease in tissue deformation strength compared with the control group (p = 0.03). CONCLUSION: Both LILT and LED can promote wound healing at the tested doses with increased tissue resistance, although its elasticity is reduced. The imprecision of the assessing instruments precluded an accurate measurement of a possible local tissue heating post irradiation and future studies are need to elucidate this effect.

[1]  Serge Mordon,et al.  Can Thermal Lasers Promote Skin Wound Healing? , 2003, American journal of clinical dermatology.

[2]  A. Pinheiro,et al.  Effect of LED phototherapy of three distinct wavelengths on fibroblasts on wound healing: a histological study in a rodent model. , 2010, Photomedicine and laser surgery.

[3]  H. Abrahamse,et al.  In vitro exposure of wounded diabetic fibroblast cells to a helium-neon laser at 5 and 16 J/cm2. , 2007, Photomedicine and laser surgery.

[4]  M. Pacheco,et al.  The effects of low-level light emitting diode on the repair process of Achilles tendon therapy in rats , 2009, Lasers in Medical Science.

[5]  Vladislav A. Kamensky,et al.  Laser-induced modification of the patellar ligament tissue: comparative study of structural and optical changes , 2010, Lasers in Medical Science.

[6]  Marisa H. G. Medeiros,et al.  Antioxidantes na manutenção do equilíbrio redox cutâneo: uso e avaliação de sua eficácia , 2007 .

[7]  M. Trelles,et al.  Red light‐emitting diode (LED) therapy accelerates wound healing post‐blepharoplasty and periocular laser ablative resurfacing , 2006, Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology.

[8]  M. Ribeiro,et al.  Ultrastructural and autoradiographical analysis show a faster skin repair in He-Ne laser-treated wounds. , 2007, Journal of photochemistry and photobiology. B, Biology.

[9]  C. Thorup,et al.  Precautions Related to Midline Sternotomy in Cardiac Surgery: A Review of Mechanical Stress Factors Leading to Sternal Complications , 2010, European journal of cardiovascular nursing : journal of the Working Group on Cardiovascular Nursing of the European Society of Cardiology.

[10]  V. Bagnato,et al.  Hardness evaluation of a dental composite polymerized with experimental LED-based devices. , 2001, Dental materials : official publication of the Academy of Dental Materials.

[11]  C. Figueira,et al.  Effect of 670-nm laser therapy and dexamethasone on tissue repair: a histological and ultrastructural study. , 2008, Photomedicine and laser surgery.

[12]  E. Munin,et al.  Comparative analysis of coherent light action (laser) versus non-coherent light (light-emitting diode) for tissue repair in diabetic rats , 2009, Lasers in Medical Science.

[13]  C. Baum,et al.  Normal Cutaneous Wound Healing: Clinical Correlation with Cellular and Molecular Events , 2005, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[14]  L. Calzà,et al.  Very low level laser therapy attenuates edema and pain in experimental models. , 2004, International journal of tissue reactions.

[15]  C. Enwemeka,et al.  Phototherapy promotes healing of chronic diabetic leg ulcers that failed to respond to other therapies , 2009, Lasers in surgery and medicine.

[16]  Vanderlei Salvador Bagnato,et al.  Photobiomodulation on the angiogenesis of skin wounds in rats using different light sources. , 2007, Photomedicine and laser surgery.

[17]  Jan Magnus Bjordal,et al.  Effect of 830 nm low-level laser therapy in exercise-induced skeletal muscle fatigue in humans , 2009, Lasers in Medical Science.

[18]  L. Calzà,et al.  Laser acupuncture for acute inflammatory, visceral and neuropathic pain relief: An experimental study in the laboratory rat. , 2010, Research in veterinary science.

[19]  Satoshi Itami,et al.  Green light emitting diodes accelerate wound healing: Characterization of the effect and its molecular basis in vitro and in vivo , 2012, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.