Determination of carbamate pesticides by a cholinesterase‐based flow injection biosensor

A potentiometric flow injection-type biosensor developed in our laboratory was used for the determination of carbamate pesticides. The principle of the biosensor is based on the inhibition of enzyme by pesticides, which is dependent on the concentration of the pesticides present. The sensor system consisted of a reactor with acetylcholinesterase immobilized on controlled pore glass and a detector with tubular H 1 -selective membrane electrode. The sensor protocol established was applied to the analysis of nine carbamate pesticides. All the pesticides at 10 -6 M inhibited the sensor enzyme, demonstrating the utility of the developed method. Treatment of the inhibited enzyme with pyridine-2-aldoxime restored nearly all of the enzyme activity allowing repeated use of the sensor. Calibration curves for carbaryl and carbofuran over the concentration range 10 -11 -10 -4 M were obtained. The lower detection limits were 10 -10 M and 10 -11 M, respectively.

[1]  P C Pandey,et al.  Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase. , 1990, Biosensors & bioelectronics.

[2]  M. Mascini,et al.  Sensitive detection of pesticides using amperometric sensors based on cobalt phthalocyanine-modified composite electrodes and immobilized cholinesterases. , 1992, Biosensors & bioelectronics.

[3]  Petr Skládal,et al.  Detection of carbamate pesticides in vegetable samples using cholinesterase-based biosensors , 1997 .

[4]  Hye-Sung Lee,et al.  Flow Injection Biosensor for the Detection of Anti-Cholinesterases , 1998 .

[5]  A. Kok,et al.  Determination of N-methylcarbamate pesticides in environmental water samples using automated on-line trace enrichment with exchangeable cartridges and high-performance liquid chromatography , 1994 .

[6]  Salvador Alegret,et al.  Amperometric determination of pesticides using a biosensor based on a polishable graphie-epoxy biocomposite , 1994 .

[7]  C. Tran-Minh,et al.  Determination of organophosphorous and carbamate insecticides by flow injection analysis. , 1992, Analytical biochemistry.

[8]  Salvador Alegret,et al.  Determination of organophosphorus and carbamate pesticides using a biosensor based on a polishable, 7,7,8,8-tetracyanoquino-dimethane-modified, graphite—epoxy biocomposite , 1997 .

[9]  E H Hansen.  Flow injection analysis: A complementary or alternative concept to biosensors. , 1994, Talanta.

[10]  D. Barceló,et al.  Determination of carbamate residues in crop samples by cholinesterase-based biosensors and chromatographic techniques , 1998 .

[11]  Petr Skládal,et al.  Detection of organophosphate and carbamate pesticides using disposable biosensors based on chemically modified electrodes and immobilized cholinesterase , 1992 .

[12]  Marek Trojanowicz,et al.  Determination of pesticides using electrochemical biosensors , 1996 .

[13]  M. Mascini,et al.  Determination of anticholinesterase pesticides in real samples using a disposable biosensor , 1997 .

[14]  Mark E. Meyerhoff,et al.  An ion-selective electrode/flow-injection analysis experiment: determination of potassium in serum , 1983 .

[15]  R. T. Krause.  Resolution, sensitivity and selectivity of a high-performance liquid chromatographic post-column fluorometric labeling technique for determination of carbamate insecticides , 1979 .

[16]  G. Cha,et al.  Fabrication of butyrylcholinesterase sensor using polyurethane-based ion-selective membranes. , 1999, Biosensors & bioelectronics.

[17]  Andrea Cagnini,et al.  DISPOSABLE RUTHENIZED SCREEN-PRINTED BIOSENSORS FOR PESTICIDES MONITORING , 1995 .

[18]  T. Noguer,et al.  Enzyme sensors for the detection of pesticides , 1993 .