A layered ultra-porous scaffold for tissue engineering, created via a hydrospinning method.

In this work we propose a new method titled "layered hydrospinning." In this method, nanofibers are being collected on top of a liquid reservoir and assembled layer-by-layer to form a 3D scaffold. The geometrical features of fabricated hydrospun scaffolds show a porosity of 99% and pores of a diameter over 100 microm. Cells were seeded during the hydrospinning process, thus achieving an initial even density of cells in the scaffold. We show that human embryonic stem cells and mouse myoblasts cultured on the scaffolds were able to infiltrate further into the scaffolds and proliferate to a greater extent, compared to conventional electrospun scaffolds collected on a plate.

[1]  Seeram Ramakrishna,et al.  Design strategies of tissue engineering scaffolds with controlled fiber orientation. , 2007, Tissue engineering.

[2]  Ulrich Buttner,et al.  Continuous yarns from electrospun fibers , 2005 .

[3]  M. Márquez,et al.  Structural studies of electrospun cellulose nanofibers , 2006 .

[4]  A. Zadhoush,et al.  Experimental investigation of the governing parameters in the electrospinning of poly(3-hydroxybutyrate) scaffolds : structural characteristics of the pores , 2010 .

[5]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part I. Traditional factors. , 2001, Tissue engineering.

[6]  J. Vacanti,et al.  Tissue engineering. , 1993, Science.

[7]  C. Wilkinson,et al.  Cells react to nanoscale order and symmetry in their surroundings , 2004, IEEE Transactions on NanoBioscience.

[8]  Gregory C Rutledge,et al.  Formation of fibers by electrospinning. , 2007, Advanced drug delivery reviews.

[9]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[10]  C. Lim,et al.  Tensile testing of a single ultrafine polymeric fiber. , 2005, Biomaterials.

[11]  Darrell H. Reneker,et al.  Structure and morphology of small diameter electrospun aramid fibers , 1995 .

[12]  Eyal Zussman,et al.  Electrostatic field-assisted alignment of electrospun nanofibres , 2001 .

[13]  Eyal Zussman,et al.  Experimental investigation of the governing parameters in the electrospinning of polymer solutions , 2004 .

[14]  Seeram Ramakrishna,et al.  A dynamic liquid support system for continuous electrospun yarn fabrication , 2007 .

[15]  E. Zussman,et al.  Formation of nanofiber crossbars in electrospinning , 2003 .

[16]  A. Mikos,et al.  Electrospun poly(epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. , 2006, Biomacromolecules.

[17]  Donald Rivin,et al.  Electrospun fiber mats: Transport properties , 1999 .

[18]  Geunhyung Kim,et al.  Highly porous 3D nanofiber scaffold using an electrospinning technique. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[19]  E. Zussman,et al.  Biohybrid nanosystems with polymer nanofibers and nanotubes , 2006, Applied Microbiology and Biotechnology.

[20]  R. Bizios,et al.  Engineering bone: challenges and obstacles , 2005, Journal of cellular and molecular medicine.

[21]  Darrell H. Reneker,et al.  Electrospinning of Nanofibers from Polymer Solutions and Melts , 2007 .

[22]  Sudha Agarwal,et al.  Improved cellular infiltration in electrospun fiber via engineered porosity. , 2007, Tissue engineering.

[23]  Il Keun Kwon,et al.  Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential. , 2005, Biomaterials.

[24]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.

[25]  Hae Woon Choi,et al.  Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation , 2007 .

[26]  Andrzej Ziabicki,et al.  Fundamentals of fibre formation: The science of fibre spinning and drawing , 1976 .