Sugar- and Nitrogen-Dependent Regulation of an Amanita muscaria Phenylalanine Ammonium Lyase Gene

ABSTRACT The cDNA of a key enzyme of secondary metabolism, phenylalanine ammonium lyase, was identified for an ectomycorrhizal fungus by differential screening of a mycorrhizal library. The gene was highly expressed in hyphae grown at low external monosaccharide concentrations, but its expression was 30-fold reduced at elevated concentrations. Gene repression was regulated by hexokinase.

[1]  U. Nehls,et al.  Carbon allocation in ectomycorrhizas: identification and expression analysis of an Amanita muscaria monosaccharide transporter. , 1998, Molecular plant-microbe interactions : MPMI.

[2]  Sally E Smith and David J Read.  Mycorrhizal Symbiosis 2nd ed , 1997 .

[3]  J. Sheen,et al.  Sugar sensing in higher plants , 1997 .

[4]  F. Romeo,et al.  Content of phenolic substances in basidiomycetes , 1997 .

[5]  B. Sundberg,et al.  Axenic mycorrhization of wild type and transgenic hybrid aspen expressing T-DNA indoleacetic acid-biosynthetic genes , 1996, Trees.

[6]  S. Wölfl,et al.  Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[7]  D. Jennings.  Stress tolerance of fungi. , 1994 .

[8]  R. Hampp,et al.  Sugar uptake by protoplasts of the ectomycorrhizal fungus, Amanita muse aria (L. ex fr.) Hooker. , 1993, The New phytologist.

[9]  D. Werner,et al.  Fast Growing Trees and Nitrogen Fixing Trees: International Conference Marburg, October 8Th-12th, 1989 , 1991 .

[10]  B. Dell,et al.  Occurrence and distribution of aspartate aminotransferases in spruce and beech ectomycorrhizas , 1990 .

[11]  K E Allen,et al.  Deoxyglucose-resistant mutants of Neurospora crassa: isolation, mapping, and biochemical characterization , 1989, Journal of bacteriology.

[12]  R. Strausberg,et al.  Nucleotide sequence of gene for phenylalanine ammonia-lyase from Rhodotorula rubra. , 1988, Nucleic acids research.

[13]  C. Reid,et al.  Interactions of nitrogen and carbon in the physiology of ectomycorrhizae , 1983 .

[14]  G. Marzluf,et al.  Regulation of L-phenylalanine ammonia-lyase by L-phenylalanine and nitrogen in Neurospora crassa , 1982, Journal of bacteriology.

[15]  G. Towers,et al.  Metabolism of aromatic acids by Polyporus hispidus , 1973 .

[16]  J. Kelly,et al.  Regulation of Carbon Metabolism in Mycelial Fungi , 1996 .

[17]  H. Ronne.  Glucose repression in fungi. , 1995, Trends in genetics : TIG.

[18]  F. Martin,et al.  Gene Expression in Roots During Ectomycorrhiza Development , 1995 .

[19]  M. Nuti,et al.  Biotechnology of ectomycorrhizae : molecular approaches , 1995 .

[20]  D. Read,et al.  Amino acids as nitrogen sources for ectomycorrhizal fungi: Utilization of individual amino acids , 1988 .

[21]  N. Minton,et al.  Complete nucleotide sequence of the Rhodosporidium toruloides gene coding for phenylalanine ammonia-lyase. , 1987, Gene.