Dietary arginine supplementation enhances immune responses to inactivated Pasteurella multocida vaccination in mice

The present study was conducted to determine the adjuvant effect of arginine in mice immunised with inactivated vaccine. Mice immunised with an inactivated Pasteurella multocida vaccine and fed diets supplemented with 0·2 % (vaccine-0·2 %) or 0·5 % (vaccine-0·5 %) arginine exhibited 100 % protection from a challenge with P. multocida serotype A (CQ2) at a dose of 4·4 × 105 colony-forming units (2LD50; median lethal dose), when compared with mice receiving no arginine supplementation. Meanwhile, antibody titres in the vaccine-0·2 % arginine group were much higher than those in the vaccine-oil adjuvant group before challenge and at 36 h post-infection. Furthermore, immunisation with the inactivated vaccine and dietary supplementation with 0·2 % arginine increased serum levels of glutathione peroxidase, in comparison with immunisation with the inactivated vaccine and an oil adjuvant. Collectively, dietary arginine supplementation confers an immunostimulatory effect in mice immunised with the inactivated P. multocida vaccine. The present results also indicate that optimal supplemental doses of arginine are 0·2–0·5 % in the mouse model.

[1]  Guoyao Wu,et al.  Effect of dietary arginine supplementation on reproductive performance of mice with porcine circovirus type 2 infection , 2011, Amino Acids.

[2]  P. Praveena,et al.  Cytokine profiles, apoptosis and pathology of experimental Pasteurella multocida serotype A1 infection in mice. , 2010, Research in veterinary science.

[3]  V. Tumiatti,et al.  Synthetic polyamines: an overview of their multiple biological activities , 2010, Amino Acids.

[4]  Guoyao Wu,et al.  Amino acids: metabolism, functions, and nutrition , 2009, Amino Acids.

[5]  Guoyao Wu,et al.  Dietary l-arginine supplementation enhances the immune status in early-weaned piglets , 2009, Amino Acids.

[6]  Sung Woo Kim,et al.  Arginine metabolism and nutrition in growth, health and disease , 2009, Amino Acids.

[7]  A. Ralph,et al.  L-arginine and vitamin D: novel adjunctive immunotherapies in tuberculosis. , 2008, Trends in microbiology.

[8]  M. Karin,et al.  The wolf in sheep's clothing: the role of interleukin-6 in immunity, inflammation and cancer. , 2008, Trends in molecular medicine.

[9]  S. Ugel,et al.  Role of arginine metabolism in immunity and immunopathology. , 2008, Immunobiology.

[10]  Peng Li,et al.  Amino acids and immune function , 2007, British Journal of Nutrition.

[11]  C. Tayade,et al.  l-Arginine stimulates intestinal intraepithelial lymphocyte functions and immune response in chickens orally immunized with live intermediate plus strain of infectious bursal disease vaccine , 2006, Vaccine.

[12]  L. Baillie,et al.  Importance of Nitric Oxide Synthase in the Control of Infection by Bacillus anthracis , 2006, Infection and Immunity.

[13]  T. N. Jaiswal,et al.  L-arginine stimulates immune response in chickens immunized with intermediate plus strain of infectious bursal disease vaccine. , 2006, Vaccine.

[14]  K. Chopra,et al.  Renal protective effect of molsidomine and L-arginine in ischemia-reperfusion induced injury in rats. , 2005, The Journal of surgical research.

[15]  G. Screaton,et al.  Characterisation of monoclonal antibodies to the TNF and TNF receptor families. , 2005, Cellular immunology.

[16]  L. Brennan,et al.  New insights into amino acid metabolism, β-cell function and diabetes , 2005 .

[17]  Lorraine Brennan,et al.  New insights into amino acid metabolism, beta-cell function and diabetes. , 2005, Clinical science.

[18]  J. Matés,et al.  Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells. , 2004, European journal of biochemistry.

[19]  Guoyao Wu,et al.  Glutathione metabolism and its implications for health. , 2004, The Journal of nutrition.

[20]  E. Battegay,et al.  Angiogenesis: mechanistic insights, neovascular diseases, and therapeutic prospects , 1995, Journal of Molecular Medicine.

[21]  V. Bronte,et al.  L-arginine metabolism in myeloid cells controls T-lymphocyte functions. , 2003, Trends in immunology.

[22]  B. Langkamp-Henken,et al.  Arginine and immunity: a unique perspective. , 2002, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[23]  J. Ruijter,et al.  Arginine deficiency affects early B cell maturation and lymphoid organ development in transgenic mice. , 2002, The Journal of clinical investigation.

[24]  G. Wu,et al.  The metabolic basis of arginine nutrition and pharmacotherapy. , 2002, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[25]  A. Visintin,et al.  Myeloid Suppressor Lines Inhibit T Cell Responses by an NO-Dependent Mechanism1 , 2002, The Journal of Immunology.

[26]  R. Johnstone,et al.  Role of TNF in lymphocyte‐mediated cytotoxicity , 2000, Microscopy research and technique.

[27]  Guoyao Wu,et al.  Arginine metabolism: nitric oxide and beyond. , 1998, The Biochemical journal.

[28]  P. Holt,et al.  Macrophage-derived nitric oxide regulates T cell activation via reversible disruption of the Jak3/STAT5 signaling pathway. , 1998, Journal of immunology.

[29]  E. Moore,et al.  Interleukin-6 in the injured patient. Marker of injury or mediator of inflammation? , 1996, Annals of surgery.

[30]  S. James.  Role of nitric oxide in parasitic infections. , 1995, Microbiological reviews.

[31]  M. Baggiolini,et al.  Interleukin-8 and the chemokine family. , 1995, International journal of immunopharmacology.

[32]  A. Barbul,et al.  Arginine enhances T-cell responses in athymic nude mice. , 1992, JPEN. Journal of parenteral and enteral nutrition.

[33]  H. Sewell,et al.  Stimulation of lymphocyte natural cytotoxicity by L-arginine , 1991, The Lancet.

[34]  C. Nathan,et al.  Role of nitric oxide synthesis in macrophage antimicrobial activity. , 1991, Current opinion in immunology.

[35]  J. Reynolds,et al.  Immunologic effects of arginine supplementation in tumor-bearing and non-tumor-bearing hosts. , 1990, Annals of surgery.

[36]  C. Avnstorp,et al.  Interleukin‐1 , 1987 .

[37]  E. Seifter,et al.  Thymic stimulatory actions of arginine. , 1980, JPEN. Journal of parenteral and enteral nutrition.