Production of lactic acid from renewable resources using electrodialysis for product recovery

Lactic acid production by a mixed culture of Lactobacillus brevis and Lactobacillus pentosus on hemicellulose from pre-treatment of wheat straw was evaluated. Different pre-treatment conditions were applied to minimise the use of alkali and oxygen in the process. Wet oxidation (no alkali) gave the highest amount of solubilised hemicellulose (measured as monosaccharides after acid hydrolysis) in particular at high oxygen pressure (25.8 g sugar/100 g straw). However, these conditions gave the lowest recovery of hemicellulose, whereas the previously found optimal conditions (alkaline wet oxidation at 12 bar oxygen) resulted in the highest hemicellulose recovery. Hence, a compromise between optimal hemicellulose recovery and solubilisation is necessary e.g. wet oxidation at 6-bar oxygen giving 23.8 g sugar/100 g straw with a hemicellulose recovery of 58%. The different hemicellulose-rich fractions were hydrolysed to fermentable sugars by the enzyme Celluclast after which these sugars were fermented to lactic acid giving up to 20 g lactic acid per 100 g straw. No inhibition from degradation products on either L. pentosus or L. brevis was observed. Hence, hemicellulose substrates look promising for lactic acid production in Denmark. Fermentation/Chapter 4

[1]  M. Iwahara,et al.  Lactic acid production by electrodialysis fermentation using immobilized growing cells. , 1987, Biotechnology and bioengineering.

[2]  M. Kleerebezem,et al.  Lactic acid bacteria as a cell factory: rerouting of carbon metabolism in Lactococcus lactis by metabolic engineering. , 2000, Enzyme and microbial technology.

[3]  M. Muir.  Physical Chemistry , 1888, Nature.

[4]  James Gomes,et al.  Axial Dispersion Model for Upflow Anaerobic Sludge Blanket Reactors , 1998, Biotechnology progress.

[5]  Masayoshi Iwahara,et al.  Novel Method of Lactic Acid Production by Electrodialysis Fermentation , 1986, Applied and environmental microbiology.

[6]  H. Gregor,et al.  Charged gels and membranes , 1976 .

[7]  José A. Manzanares,et al.  Effects of temperature and ion transport on water splitting in bipolar membranes , 1992 .

[8]  Ulrich Kulozik,et al.  Rapid lactic acid production at high cell concentrations in whey ultrafiltrate by Lactobacillus helveticus , 1999 .

[9]  J. Caro,et al.  Basic Principles of Membrane Technology , 1998 .

[10]  A. M.W.,et al.  Fermented co-products and fermented compound diets for pigs : a review , 1999 .

[11]  Kiyoshi Ishii,et al.  Continuous Glucose Fermentation for Lactic Acid Production: Recovery of Acid by Electrodialysis , 1987 .

[12]  R. Rota,et al.  Lactic acid production by electrodialysis Part II: Modelling , 2013 .

[13]  M. Cheryan,et al.  Lactic acid from cheese whey permeate. Productivity and economics of a continuous membrane bioreactor , 1995, Applied Microbiology and Biotechnology.

[14]  D. Pyle,et al.  Emulsion liquid membrane extraction of lactic acid from aqueous solutions and fermentation broth , 1993, Biotechnology and bioengineering.

[15]  J. Rincón,et al.  SELECTION OF A CATION EXCHANGE RESIN TO PRODUCE LACTIC ACID SOLUTIONS FROM WHEY FERMENTATION BROTHS , 1997 .

[16]  G. Jonsson,et al.  Concentration polarization in a reverse osmosis test cell , 1977 .

[17]  J. Villadsen.  Lactic Acid Production , 2000 .

[18]  P. Boyaval,et al.  Lactose continuous fermentation with cells recycled by ultrafiltration and lactate separation by electrodialysis: model identification , 1989, Applied Microbiology and Biotechnology.

[19]  Viatcheslav Freger,et al.  Separation of concentrated organic/inorganic salt mixtures by nanofiltration , 2000 .

[20]  J. Lunt.  Large-scale production, properties and commercial applications of polylactic acid polymers , 1998 .

[21]  W. Holzapfel,et al.  Lactic acid bacteria of foods and their current taxonomy. , 1997, International journal of food microbiology.

[22]  H. Brunner,et al.  Integrated bioprocess for the simultaneous production of lactic acid and dairy sewage treatment , 1998 .