Elastic light scattering from nucleated blood cells: rapid numerical analysis.

A model is presented to calculate the light-scatter properties of nucleated blood cells which are mimicked by two concentric spheres. The light-scatter characteristics were derived from the Rayleigh-Debye-Gans approximation and by simultaneous application of field averaging of the internal field and modification of the propagation constant inside the different cellular compartments. It is shown that the results obtained with this simple model are similar to those obtained with more complex and more exact theories. In addition it is demonstrated that simultaneous detection of the light-scatter intensities in the forward-, lateral-, and backscatter directions is required to optimize the detection of different cell types in heterogeneous populations of nucleated blood cells.

[1]  M. Hartmann,et al.  Light scattering by small particles. Von H. C. VANDE HULST. New York: Dover Publications, Inc. 1981. Paperback, 470 S., 103 Abb. und 46 Tab., US $ 7.50 , 1984 .

[2]  A. R. Jones.  Calculation of the ratios of complex Riccati-Bessel functions for Mie scattering , 1983 .

[3]  F. Bryant,et al.  Changes in total light scattering and absorption caused by changes in particle conformation. , 1968, Journal of theoretical biology.

[4]  J. Steinkamp,et al.  A new multiparameter separator for microscopic particles and biological cells. , 1973, The Review of scientific instruments.

[5]  S. Asano,et al.  Light scattering by a spheroidal particle. , 1975, Applied optics.

[6]  Irene A. Stegun,et al.  Handbook of Mathematical Functions. , 1966 .

[7]  M. Kerker,et al.  Elastic and inelastic light scattering in flow cytometry. , 1983, Cytometry.

[8]  G. van den Engh,et al.  Light scattering properties of murine hemopoietic cells. , 1980, Blood cells.

[9]  Milton Kerker,et al.  The Scattering of Light and Other Electromagnetic Radiation ~Academic , 1969 .

[10]  Milton Kerker,et al.  Light scattering by structured spheres , 1978 .

[11]  R. A. Meyer,et al.  Light scattering from nucleated biological cells. , 1975, Biophysical journal.

[12]  W. Wiscombe.  Improved Mie scattering algorithms. , 1980, Applied optics.

[13]  T. Lindmo,et al.  Flow cytometry and sorting , 1979 .

[14]  D. S. Coffey,et al.  The application of perpendicular and forward light scatter to assess nuclear and cellular morphology. , 1984, Cytometry.

[15]  R. Snyderman,et al.  Rapid changes in light scattering from human polymorphonuclear leukocytes exposed to chemoattractants. Discrete responses correlated with chemotactic and secretory functions. , 1984, The Journal of clinical investigation.

[16]  Koichi Shimizu.  Modification of the Rayleigh–Debye approximation , 1983 .

[17]  R. Snyderman,et al.  Rapid Changes in Light Scattering from Human , 1984 .

[18]  H. Micklem,et al.  Right angle light scatter: a necessary parameter in flow cytofluorimetric analysis of human peripheral blood mononuclear cells. , 1983, Journal of immunological methods.

[19]  Milton Kerker,et al.  Scattering of Electromagnetic Waves from Two Concentric Spheres , 1951 .

[20]  G C Salzman,et al.  A flow-system multiangle light-scattering instrument for cell characterization. , 1975, Clinical chemistry.

[21]  H. V. Hulst.  Light Scattering by Small Particles , 1957 .

[22]  M. Melamed,et al.  Size and refractive index dependence of simple forward angle scattering measurements in a flow system using sharply-focused illumination. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[23]  M. Loken,et al.  Flow cytometry as an analytical and preparative tool in immunology. , 1982, Journal of immunological methods.

[24]  G C Salzman,et al.  Cell classification by laser light scattering: identification and separation of unstained leukocytes. , 1975, Acta cytologica.

[25]  C F Bohren,et al.  Absorption by a sphere: a simple approximation. , 1983, Applied optics.

[26]  P. Barber,et al.  Scattering of electromagnetic waves by arbitrarily shaped dielectric bodies. , 1975, Applied optics.

[27]  Herbert Begemann,et al.  Atlas of Clinical Hematology , 1979, Springer Berlin Heidelberg.

[28]  P. F. Mullaney,et al.  Light scattering from coated spheres: model for biological cells. , 1972, Applied optics.