Changes in blood lymphocyte populations in experimental bowel allograft rejection

Abstract  The aim of this study is to measure percentages of lymphocyte populations and IL‐2R cellular expression in peripheral blood during the rejection of a small bowel allograft (SBA) in the rat. Thirty rats were allotted to three groups: A, control, no transplantation (Tx); B, rats receiving an orthotopic SBA; C, similar SBA but with thymostimulin (TP‐1) administered before Tx, aimed at increasing the intensity of and accelerating rejection. The percentages of CD19, CD5, CD4 and CD8 cells and of IL‐2R were determined when rejection was present. Rejection appeared in rats in group B between days 11 and 26 post‐Tx and in group C between days 6 and 7 post‐Tx (P < 0.001). In both B and C groups, CD.5 and CD4 cells decreased (P < 0.005) and CD8 cells increased (P < 0.001). A correlation between CD8 and IL‐2R content was found (P < 0.05). In group C, earliness of rejection correlated with the percentage of CD8 cells (P < 0.05) and the intensity of rejection with numbers of CD8 and CD19 cells (P< 0.05).

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