Effects of sub-lethal high-pressure homogenization treatment on the outermost cellular structures and the volatile-molecule profiles of two strains of probiotic lactobacilli

Applying sub-lethal levels of high-pressure homogenization (HPH) to lactic acid bacteria has been proposed as a method of enhancing some of their functional properties. Because the principal targets of HPH are the cell-surface structures, the aim of this study was to examine the effect of sub-lethal HPH treatment on the outermost cellular structures and the proteomic profiles of two known probiotic bacterial strains. Moreover, the effect of HPH treatment on the metabolism of probiotic cells within a dairy product during its refrigerated storage was investigated using SPME-GC-MS. Transmission electron microscopy was used to examine the microstructural changes in the outermost cellular structures due to HPH treatment. These alterations may be involved in the changes in some of the technological and functional properties of the strains that were observed after pressure treatment. Moreover, the proteomic profiles of the probiotic strains treated with HPH and incubated at 37°C for various periods showed different peptide patterns compared with those of the untreated cells. In addition, there were differences in the peaks that were observed in the low-mass spectral region (2000–3000 Da) of the spectral profiles of the control and treated samples. Due to pressure treatment, the volatile-molecule profiles of buttermilk inoculated with treated or control cells and stored at 4°C for 30 days exhibited overall changes in the aroma profile and in the production of molecules that improved its sensory profile, although the two different species imparted specific fingerprints to the product. The results of this study will contribute to understanding the changes that occur in the outermost cellular structures and the metabolism of LAB in response to HPH treatment. The findings of this investigation may contribute to elucidating the relationships between these changes and the alterations of the technological and functional properties of LAB induced by pressure treatment.

[1]  G. Tabanelli,et al.  Effect of a sublethal high‐pressure homogenization treatment on the fatty acid membrane composition of probiotic lactobacilli , 2014, Letters in applied microbiology.

[2]  Z. Zdráhal,et al.  The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling. , 2013, Rapid communications in mass spectrometry : RCM.

[3]  G. Tabanelli,et al.  Effect of sub-lethal high pressure homogenization treatments on the in vitro functional and biological properties of lactic acid bacteria , 2013 .

[4]  G. Tabanelli,et al.  Effect of a non-lethal High Pressure Homogenization treatment on the in vivo response of probiotic lactobacilli. , 2012, Food microbiology.

[5]  Gilbert GREUB,et al.  Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. , 2012, FEMS microbiology reviews.

[6]  K. Aryana,et al.  Some low homogenization pressures improve certain probiotic characteristics of yogurt culture bacteria and Lactobacillus acidophilus LA-K. , 2011, Journal of dairy science.

[7]  A. Urbani,et al.  MALDI-TOF mass spectrometry proteomic phenotyping of clinically relevant fungi. , 2011, Molecular bioSystems.

[8]  N. Innocente,et al.  Feasibility of the SPME method for the determination of the aroma retention capacity of proteose–peptone milk protein fraction at different pH values , 2011 .

[9]  Francesca Patrignani,et al.  Suitability of high pressure-homogenized milk for the production of probiotic fermented milk containing Lactobacillus paracasei and Lactobacillus acidophilus , 2009, Journal of Dairy Research.

[10]  L. Vannini,et al.  Effect of a pre-treatment of milk with high pressure homogenization on yield as well as on microbiological, lipolytic and proteolytic patterns of "Pecorino" cheese. , 2008, International journal of food microbiology.

[11]  G. Vinderola,et al.  Suitability of whey and buttermilk for the growth and frozen storage of probiotic lactobacilli , 2008 .

[12]  F. Patrignani,et al.  Effects of sub-lethal concentrations of hexanal and 2-(E)-hexenal on membrane fatty acid composition and volatile compounds of Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Escherichia coli. , 2008, International journal of food microbiology.

[13]  F. Patrignani,et al.  Probiotic Crescenza cheese containing Lactobacillus casei and Lactobacillus acidophilus manufactured with high-pressure homogenized milk. , 2008, Journal of dairy science.

[14]  A. Palva,et al.  Isolation of surface (S) layer protein carrying Lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues. , 2007, Veterinary microbiology.

[15]  F. Patrignani,et al.  Effect of high-pressure homogenization, nonfat milk solids, and milkfat on the technological performance of a functional strain for the production of probiotic fermented milks. , 2007, Journal of dairy science.

[16]  M. Zagorec,et al.  Protein synthesis in lactic acid and pathogenic bacteria during recovery from a high pressure treatment. , 2007, Research in microbiology.

[17]  F. Patrignani,et al.  Effects of high pressure homogenization on the activity of lysozyme and lactoferrin against Listeria monocytogenes , 2007 .

[18]  L. Vannini,et al.  Effect of high pressure homogenisation of milk on cheese yield and microbiology, lipolysis and proteolysis during ripening of Caciotta cheese , 2006, Journal of Dairy Research.

[19]  C. Michiels,et al.  High-Pressure Homogenization as a Non-Thermal Technique for the Inactivation of Microorganisms , 2006, Critical reviews in microbiology.

[20]  T. Klaenhammer,et al.  Functional Analysis of Putative Adhesion Factors in Lactobacillus acidophilus NCFM , 2005, Applied and Environmental Microbiology.

[21]  A. Palva,et al.  Lactobacillus surface layers and their applications. , 2005, FEMS microbiology reviews.

[22]  A. Palva,et al.  surface layers and their applications , 2005 .

[23]  J. Frece,et al.  Importance of S‐layer proteins in probiotic activity of Lactobacillus acidophilus M92 , 2005, Journal of applied microbiology.

[24]  L. Vannini,et al.  Interactions between high pressure homogenization and antimicrobial activity of lysozyme and lactoperoxidase. , 2004, International journal of food microbiology.

[25]  S. Salminen,et al.  Effects of Polyunsaturated Fatty Acids in Growth Medium on Lipid Composition and on Physicochemical Surface Properties of Lactobacilli , 2004, Applied and Environmental Microbiology.

[26]  M. Kaláb,et al.  Disruption of Lactobacillus delbrueckii ssp. bulgaricus 11842 cells for lactose hydrolysis in dairy products: a comparison of sonication, high-pressure homogenization and bead milling , 2001 .

[27]  C. Vinderola,et al.  Viability of probiotic (Bifidobacterium, Lactobacillus acidophilus and Lactobacillus casei) and nonprobiotic microflora in Argentinian Fresco cheese. , 2000, Journal of dairy science.

[28]  Juliane Floury,et al.  Effect of high-pressure homogenization on droplet size distributions and rheological properties of model oil-in-water emulsions , 2000 .

[29]  S. Salminen,et al.  The ability of probiotic bacteria to bind to human intestinal mucus. , 1998, FEMS microbiology letters.

[30]  M. Sinigaglia,et al.  Increased cellular fatty acid desaturation as a possible key factor in thermotolerance in Saccharomyces cerevisiae. , 1997, Canadian journal of microbiology.

[31]  R. Lanciotti,et al.  Effect of high hydrostatic pressure and high pressure homogenization on the enantioselectivity of microbial reductions , 1996 .

[32]  R. Weselake,et al.  Phosphatidate phosphatases of mammals, yeast, and higher plants , 1996, Lipids.

[33]  E. Skolnik,et al.  Finally, some signaling molecules find a home in yeast , 1996, Nature Biotechnology.

[34]  N J Russell,et al.  Membranes as a target for stress adaptation. , 1995, International journal of food microbiology.

[35]  G. Tabanelli,et al.  Combined effects of high pressure homogenization treatment and citral on microbiological quality of apricot juice. , 2013, International journal of food microbiology.

[36]  F. Patrignani,et al.  Effects of milk high pressure homogenization on biogenic amine accumulation during ripening of ovine and bovine Italian cheeses , 2007 .

[37]  A. Basson,et al.  Evaluation of Adherence, Hydrophobicity, Aggregation, and Biofilm Development of Flavobacterium johnsoniae-Like Isolates , 2007, Microbial Ecology.

[38]  F. Patrignani,et al.  Potential of high pressure homogenization in the control and enhancement of proteolytic and fermentative activities of some Lactobacillus species , 2007 .

[39]  S. Salminen,et al.  The influence of polyunsaturated fatty acids on probiotic growth and adhesion. , 2001, FEMS microbiology letters.