论文信息 - Effect of Sugars on Rooting of Shoots of Japanese Persimmon Propagated in vitro

Effect of Sugars on Rooting of Shoots of Japanese Persimmon Propagated in vitro

The effect of different concentrations of sucrose, glucose and fructose on in vitro rooting of shoots of Japanese persimmon that had been induced from selected rootstocks was studied. When the shoots of Japanese persimmon strain “No.3” were cultured on autoclaved half-strength Murashige and Skoog medium (MS medium) containing 0.2M fructose, roots were induced in 94% of the shoots after 45 days of culture whereas only 30% of rooting was obtained on media containing the same concentration of sucrose or glucose. However, filter-sterilized fructose had no stimulative effect on rooting. The autoclaved medium containing 0.2M fructose showed consistently high percentages of rooting (80%<) of the shoots for 9 rootstock strains tested, whereas only 3 strains responded to the culture on medium containing 0.1M sucrose following 250mg/l IBA pretreatment.

H. Kagami

[1] A. Sugiura,et al. Micropropagation of Japanese persimmon (Diospyros kaki L.) , 1992 .

[2] H. Fukui,et al. Varietal Differences in Rooting Ability of In Vitro Subcultured Japanese Persimmon Shoots , 1992 .

[3] H. Fukui,et al. Varietal differences in Shoot Tip Culture of Japanese Persimmon , 1990 .

[4] R. Tao,et al. In Vitro Shoot Proliferation and Rooting of Several Japanese Persimmon Cultivars , 1989 .

[5] H. Fukui,et al. Shoot Tip Culture of Japanese Persimmon (Diospyros kaki Thunb.) , 1989 .

[6] R. Tao,et al. In Vitro Propagation of Japanese Persimmon , 1986, HortScience.

[7] C. Chong,et al. Carbon nutrition of Ottawa 3 apple rootstock during stages of in vitro propagation , 1985 .

[8] D. J. James,et al. Shoot and root initiation in vitro in the apple rootstock M.9 and the promotive effects of phloroglucinol , 1981 .

[9] D. J. James,et al. Rapid in Vitro Rooting of the Apple Rootstock M.9 , 1979 .

[10] O. Jones,et al. The successful propagation in vitro of two rootstocks of Prunus: the plum rootstock Pixy (P. insititia) and the cherry rootstock F 12/1 (P. avium). , 1979 .

[11] O. Jones,et al. Propagation in Vitro of M.26 Apple Rootstocks , 1977 .

[12] R. Berry,et al. Acid-catalyzed degradation of d-fructose , 1967 .

[13] F. Skoog,et al. A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .