Nucleic Acid Homologies Among Oxidase-Negative Moraxella Species

The deoxyribonucleic acid (DNA) base composition and DNA homologies of more than 40 strains of oxidase-negative Moraxella species were determined. These bacteria have also been identified as belonging to the Mima-Herellea-Acinetobacter group and the Bacterium anitratum group, as well as to several other genera including Achromobacter and Alcaligenes. The DNA base content of these strains ranged from 40 to 46% guanine plus cytosine. DNA-DNA competition experiments distinguished five groups whose members were determined by showing 50% or more homology to one of the reference strains: B. anitratum type B5W, Achromobacter haemolyticus var. haemolyticus, Alcaligenes haemolysans, Achromobacter metalcaligenes, and Moraxella lwoffi. A sixth group comprised those strains showing less than 50% homology to any of the reference strains. Negligible homology was found between strains of oxidase-negative and oxidase-positive Moraxella species in DNADNA competition experiments. However, evidence of a distant relationship between the two groups was obtained in competition experiments by using ribosomal ribonucleic acid.

[1]  A. Henderson.  TheMoraxella lwoffi group of bacteria; a review , 2005, Antonie van Leeuwenhoek.

[2]  M. Córdova,et al.  [The Moraxella lwoffi group of bacteria (author's transl)]. , 1976, Revista medica de Chile.

[3]  M. Doudoroff,et al.  A Study of the Moraxella Group II. Oxidative-negative Species (Genus Acinetobacter) , 1968, Journal of bacteriology.

[4]  J. Johnson,et al.  Deoxyribonucleic Acid Homology in Bacterial Taxonomy: Effect of Incubation Temperature on Reaction Specificity , 1968, Journal of Bacteriology.

[5]  M. Doudoroff,et al.  Study of the Moraxella Group I. Genus Moraxella and the Neisseria catarrhalis Group , 1968, Journal of bacteriology.

[6]  B. Mccarthy,et al.  Comparative Study of Ribosomal Ribonucleic Acid Cistrons in Enterobacteria and Myxobacteria , 1967, Journal of bacteriology.

[7]  Hideo Takahashi,et al.  Species specificity of the ribosomal RNA cistrons in bacteria , 1967 .

[8]  R. E. Click,et al.  The isolation of ribonucleic acid from plant, bacterial or animal cells. , 1966, Biochimica et biophysica acta.

[9]  R. Doi,et al.  Heterogeneity of the Conserved Ribosomal Ribonucleic Acid Sequences of Bacillus subtilis , 1966, Journal of bacteriology.

[10]  D T Denhardt,et al.  A membrane-filter technique for the detection of complementary DNA. , 1966, Biochemical and biophysical research communications.

[11]  D. Dubnau,et al.  Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[12]  S. Spiegelman,et al.  A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. , 1965, Journal of molecular biology.

[13]  W. Mannheim,et al.  On the classification and nomenclature of some nonmotile and coccoid diplobacteria, exhibiting the properties of Achromobacteriaceae , 1963 .

[14]  B. Mccarthy,et al.  An approach to the measurement of genetic relatedness among organisms. , 1963, Proceedings of the National Academy of Sciences of the United States of America.

[15]  J. Marmur.  [100] A procedure for the isolation of deoxyribonucleic acid from microorganisms , 1963 .

[16]  P. Doty,et al.  Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. , 1962, Journal of molecular biology.

[17]  P. Doty,et al.  Thermal renaturation of deoxyribonucleic acids. , 1961, Journal of molecular biology.

[18]  S. D. Henriksen.  Moraxella: classification and taxonomy. , 1952, Journal of general microbiology.

[19]  '. Paulbaumann.  Isolation of Acinetobacter from Soil and Water , 2022 .

[20]  R. Doil,et al.  Conservation of Ribosomal and Messenger Ribonucleic Acid Cistrons in Bacillus Species , 2022 .