A strategy for accurate detection of glucose in human serum and whole blood based on an upconversion nanoparticles-polydopamine nanosystem

The accurate detection of blood glucose is of critical importance in the diagnosis and management of diabetes and its complications. Herein, we report a novel strategy based on an upconversion nanoparticles-polydopamine (UCNPs-PDA) nanosystem for the accurate detection of glucose in human serum and whole blood through a simple blending of test samples with ligand-free UCNPs, dopamine, and glucose oxidase (GOx). Owing to the high affinity of lanthanide ions exposed on the surface of ligand-free UCNPs, dopamine monomers could spontaneously attach to the UCNPs and further polymerize to form a PDA shell, resulting in a remarkable upconversion luminescence (UCL) quenching (97.4%) of UCNPs under 980-nm excitation. Such UCL quenching can be effectively inhibited by H2O2 produced from the GOx/glucose enzymatic reaction, thus enabling the detection of H2O2 or glucose based on the UCL quenching/inhibition bioassay. Owing to the highly sensitive UCL response and background-free interference of the UCNPs-PDA nanosystem, we achieved a sensitive, selective, and high-throughput bioassay for glucose in human serum and whole blood, thereby revealing the great potential of the UCNPs-PDA nanosystem for the accurate detection of blood glucose or other H2O2-generated biomolecules in clinical bioassays.

[1]  Classification and diagnosis of diabetes mellitus and other categories of glucose intolerance. National Diabetes Data Group. , 1979, Diabetes.

[2]  W. Tseng,et al.  Formation of fluorescent polydopamine dots from hydroxyl radical-induced degradation of polydopamine nanoparticles. , 2015, Physical chemistry chemical physics : PCCP.

[3]  Ru-Qin Yu,et al.  MnO2-Nanosheet-Modified Upconversion Nanosystem for Sensitive Turn-On Fluorescence Detection of H2O2 and Glucose in Blood. , 2015, ACS applied materials & interfaces.

[4]  Markus P. Hehlen,et al.  Hexagonal Sodium Yttrium Fluoride Based Green and Blue Emitting Upconversion Phosphors. , 2004 .

[5]  Dayong Jin,et al.  Controlling upconversion nanocrystals for emerging applications. , 2015, Nature nanotechnology.

[6]  R. Nitschke,et al.  Quantum dots versus organic dyes as fluorescent labels , 2008, Nature Methods.

[7]  Wei Feng,et al.  Resonance Energy Transfer in Upconversion Nanoplatforms for Selective Biodetection. , 2017, Accounts of chemical research.

[8]  Sook Hee Ku,et al.  Human endothelial cell growth on mussel-inspired nanofiber scaffold for vascular tissue engineering. , 2010, Biomaterials.

[9]  A. Heller,et al.  Electrochemical Glucose Sensors and Their Applications in Diabetes Management , 2008 .

[10]  Ping Huang,et al.  Inorganic lanthanide nanoprobes for background-free luminescent bioassays , 2015, Science China Materials.

[11]  S. Yao,et al.  Sensitive fluorescent detection of H2O2 and glucose in human serum based on inner filter effect of squaric acid-iron(III) on the fluorescence of upconversion nanoparticle. , 2017, Talanta.

[12]  Zhihong Liu,et al.  Construction of an upconversion nanoprobe with few-atom silver nanoclusters as the energy acceptor. , 2015, Angewandte Chemie.

[13]  Wei Wang,et al.  A high-throughput colorimetric assay for glucose detection based on glucose oxidase-catalyzed enlargement of gold nanoparticles. , 2015, Nanoscale.

[14]  Xiao Dong Chen,et al.  Glucose oxidase: natural occurrence, function, properties and industrial applications , 2008, Applied Microbiology and Biotechnology.

[15]  Jianhua Hao,et al.  Ultrasensitive Detection of Ebola Virus Oligonucleotide Based on Upconversion Nanoprobe/Nanoporous Membrane System. , 2016, ACS nano.

[16]  P. J. Huang,et al.  Hydrogen peroxide displacing DNA from nanoceria: mechanism and detection of glucose in serum. , 2015, Journal of the American Chemical Society.

[17]  Meng Wang,et al.  Lanthanide-doped upconversion nanoparticles electrostatically coupled with photosensitizers for near-infrared-triggered photodynamic therapy. , 2014, Nanoscale.

[18]  Vinod P. Shah,et al.  Bioanalytical Method Validation—A Revisit with a Decade of Progress , 2000, Pharmaceutical Research.

[19]  Datao Tu,et al.  A facile “ship-in-a-bottle” approach to construct nanorattles based on upconverting lanthanide-doped fluorides , 2015, Nano Research.

[20]  S. Clarke,et al.  A history of blood glucose meters and their role in self-monitoring of diabetes mellitus , 2012, British journal of biomedical science.

[21]  Jing Wang,et al.  A novel strategy for improving upconversion luminescence of NaYF4:Yb, Er nanocrystals by coupling with hybrids of silver plasmon nanostructures and poly(methyl methacrylate) photonic crystals , 2013, Nano Research.

[22]  Igor L. Medintz,et al.  Quantum dot bioconjugates for imaging, labelling and sensing , 2005, Nature materials.

[23]  Zhihong Liu,et al.  Biosensing platform based on fluorescence resonance energy transfer from upconverting nanocrystals to graphene oxide. , 2011, Angewandte Chemie.

[24]  Guohua Liu,et al.  Fluorescent gold nanoclusters based photoelectrochemical sensors for detection of H2O2 and glucose. , 2015, Biosensors & bioelectronics.

[25]  Yongsheng Liu,et al.  Lanthanide-doped semiconductor nanocrystals: electronic structures and optical properties , 2015, Science China Materials.

[26]  G. A. Swan.  CHEMICAL STRUCTURE OF MELANINS , 1963, Annals of the New York Academy of Sciences.

[27]  Geoffrey A Ozin,et al.  Synthesis of ligand-free colloidally stable water dispersible brightly luminescent lanthanide-doped upconverting nanoparticles. , 2011, Nano letters.

[28]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[29]  Lehui Lu,et al.  Polydopamine and its derivative materials: synthesis and promising applications in energy, environmental, and biomedical fields. , 2014, Chemical reviews.

[30]  Sung Min Kang,et al.  One-step modification of superhydrophobic surfaces by a mussel-inspired polymer coating. , 2010, Angewandte Chemie.

[31]  Visual fluorescence detection of H2O2 and glucose based on "molecular beacon"-hosted Hoechst dyes. , 2015, The Analyst.

[32]  Dalong Ni,et al.  Upconversion nano-photosensitizer targeting into mitochondria for cancer apoptosis induction and cyt c fluorescence monitoring , 2016, Nano Research.

[33]  Peter A. Tanner,et al.  Upconversion for White Light Generation by a Single Compound. , 2010 .

[34]  Hui Li,et al.  NiO nanoparticles modified with 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin: promising peroxidase mimetics for H2O2 and glucose detection. , 2015, Biosensors & bioelectronics.

[35]  Huafeng Yang,et al.  Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene. , 2009, Analytical chemistry.

[36]  Danke Xu,et al.  Bioinspired polydopamine nanospheres: a superquencher for fluorescence sensing of biomolecules , 2014 .

[37]  Z. Trajanoski,et al.  Accuracy of home blood glucose meters during hypoglycemia , 1996, Diabetes Care.

[38]  Wei Zheng,et al.  Lanthanide-doped upconversion nano-bioprobes: electronic structures, optical properties, and biodetection. , 2015, Chemical Society reviews.

[39]  Hans H Gorris,et al.  Photon-upconverting nanoparticles for optical encoding and multiplexing of cells, biomolecules, and microspheres. , 2013, Angewandte Chemie.

[40]  G. Rossi,et al.  Diagnosis and Classification of Diabetes Mellitus The information that follows is based largely on the reports of the Expert Committee on the Diagnosis and Classification of Diabetes (Diabetes Care 20:1183–1197, 1997, and Diabetes Care 26:3160–3167, 2003). , 2008, Diabetes Care.

[41]  Zhihong Liu,et al.  A Rationally Designed Upconversion Nanoprobe for in Vivo Detection of Hydroxyl Radical. , 2015, Journal of the American Chemical Society.

[42]  Ping Huang,et al.  Lanthanide-doped LiLuF(4) upconversion nanoprobes for the detection of disease biomarkers. , 2014, Angewandte Chemie.

[43]  Yongsheng Liu,et al.  Breakdown of crystallographic site symmetry in lanthanide-doped NaYF4 crystals. , 2013, Angewandte Chemie.

[44]  Ping Huang,et al.  Ultrasensitive Luminescent In Vitro Detection for Tumor Markers Based on Inorganic Lanthanide Nano‐Bioprobes , 2016, Advanced science.

[45]  Juan Tang,et al.  Simultaneous detection of hydrogen peroxide and glucose in human serum with upconversion luminescence. , 2015, Biosensors & bioelectronics.

[46]  Mingying Yang,et al.  NIR-induced highly sensitive detection of latent fingermarks by NaYF4:Yb,Er upconversion nanoparticles in a dry powder state , 2015, Nano Research.

[47]  P. Zimmet,et al.  Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus. Provisional report of a WHO Consultation , 1998, Diabetic medicine : a journal of the British Diabetic Association.

[48]  R. Yu,et al.  Fabrication of a LRET-based upconverting hybrid nanocomposite for turn-on sensing of H2O2 and glucose. , 2016, Nanoscale.

[49]  Liyuan Liu,et al.  Down-shifting luminescence of water soluble NaYF4:Eu3+@Ag core-shell nanocrystals for fluorescence turn-on detection of glucose , 2016, Science China Materials.

[50]  O. Wolfbeis,et al.  Luminescent sensing of oxygen using a quenchable probe and upconverting nanoparticles. , 2011, Angewandte Chemie.