Metal-recognition by repeating polypeptides

Attachment of proteins to metal surfaces has the potential to improve our understanding of protein adhesion and has applications in sensor technology. Repeating polypeptides able to bind to metallic gold or chromium were selected from a population of approximately 5 million different polypeptides. Each polypeptide contained several direct repeats of identical peptide units 14 or 28 amino acids long. The metal-recognizing polypeptides were found to retain their binding properties when freed from the protein used to select them. One gold-binding polypeptide's avidity for gold was found to be dependent on the number of repeats and the presence of salt.

[1]  A. Ménez,et al.  Direct expression in E. coli of a functionally active protein A--snake toxin fusion protein. , 1989, Protein engineering.

[2]  D. Handley,et al.  Colloidal Gold: A Pluripotent Receptor Probe , 1983, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[3]  S. Brown,et al.  Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[4]  D. Lockshon,et al.  Sites of reaction of Escherichia coli DNA gyrase on pBR322 in vivo as revealed by oxolinic acid-induced plasmid linearization. , 1985, Journal of molecular biology.

[5]  J. Vieira,et al.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. , 1982, Gene.

[6]  J. Watterson.  Artifacts resembling budding bacteria produced in placer-gold amalgams by nitric acid leaching , 1994 .

[7]  H. Nojima,et al.  High efficiency transformation of Escherichia coli with plasmids. , 1990, Gene.

[8]  Stanley Brown,et al.  Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli , 1987, Cell.

[9]  F. Caruso,et al.  Immobilisation of IgG onto gold surfaces and its interaction with anti-IgG studied by surface plasmon resonance. , 1994, Journal of immunological methods.

[10]  S. Pääbo,et al.  DNA damage promotes jumping between templates during enzymatic amplification. , 1990, The Journal of biological chemistry.

[11]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[12]  E. E. Kim,et al.  Reaction mechanism of alkaline phosphatase based on crystal structures. Two-metal ion catalysis. , 1991, Journal of molecular biology.

[13]  T. Creighton.  Proteins: Structures and Molecular Properties , 1986 .

[14]  P. Fenter,et al.  Self-Assembly of n-Alkyl Thiols as Disulfides on Au(111) , 1994, Science.

[15]  I. Wilson,et al.  Enzymic parameters: measurement of V and Km. , 1971, Biochimica et Biophysica Acta.

[16]  E. Atkins,et al.  Chemical sequence control of beta-sheet assembly in macromolecular crystals of periodic polypeptides. , 1994, Science.

[17]  H. Schägger,et al.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. , 1987, Analytical biochemistry.

[18]  A. Fire,et al.  Rolling replication of short DNA circles. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[19]  G M Whitesides,et al.  A self-assembled monolayer for the binding and study of histidine-tagged proteins by surface plasmon resonance. , 1996, Analytical chemistry.

[20]  M. Hofnung,et al.  Linker mutagenesis in the gene of an outer membrane protein of escherichia coli, LamB , 1984, Journal of cellular biochemistry.

[21]  C. Richardson,et al.  The effect of the T7 and Escherichia coli DNA-binding proteins at the replication fork of bacteriophage T7. , 1988, The Journal of biological chemistry.

[22]  A. Ménez,et al.  Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities. , 1992, Protein engineering.

[23]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .

[24]  A. C. Chang,et al.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid , 1978, Journal of bacteriology.

[25]  S. G. Smith,et al.  The amino acid sequence in a fraction of the fibroin of Bombyx mori. , 1957, The Biochemical journal.

[26]  E. McGroarty,et al.  Quantitation of metal cations bound to membranes and extracted lipopolysaccharide of Escherichia coli. , 1983, Biochemistry.

[27]  R. Hoess,et al.  Tendamistat as a scaffold for conformationally constrained phage peptide libraries. , 1995, Journal of molecular biology.