Pathogenicity of an EnterotoxigenicEscherichia coli Hemolysin (hlyA) Mutant in Gnotobiotic Piglets

ABSTRACT Pigs infected with hemolytic F4+ strains of enterotoxigenic Escherichia coli often develop septicemia secondary to intestinal infection. We tested the hypothesis that inactivation of hemolysin would reduce the ability of F4+enterotoxigenic E. coli to cause septicemia in swine following oral inoculation. Inactivation of the hemolysin structural gene (hlyA) did not decrease the incidence of septicemia in the gnotobiotic piglet model.

[1]  D. Francis,et al.  Distribution of K88 Escherichia coli-adhesive and nonadhesive phenotypes among pigs of four breeds. , 1997, Veterinary microbiology.

[2]  R. Warren,et al.  Molecular characterization of the tia invasion locus from enterotoxigenic Escherichia coli , 1996, Infection and immunity.

[3]  A. Andersen,et al.  Lung and Nasal Lesions Caused by a Swine Chlamydial Isolate in Gnotobiotic Pigs , 1996, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[4]  R. Welch,et al.  Battling against host phagocytes: the wherefore of the RTX family of toxins? , 1995, Infectious agents and disease.

[5]  L. Siegfried,et al.  Serum response of Escherichia coli strains causing dyspepsia and urinary tract infection: relation to alpha-hemolysin production and O type , 1995, Infection and immunity.

[6]  A. Brauner,et al.  P-fimbriation and haemolysin production are the most important virulence factors in diabetic patients with Escherichia coli bacteraemia: a multivariate statistical analysis of seven bacterial virulence factors. , 1995, The Journal of infection.

[7]  C. Gyles.  Escherichia coli in Domestic Animals and Humans , 1994 .

[8]  H. Moon,et al.  Vaccines for preventing enterotoxigenic Escherichia coli infections in farm animals , 1993, Vaccine.

[9]  W. Thomas,et al.  A heterologous membrane protein domain fused to the C-terminal ATP-binding domain of HlyB can export Escherichia coli hemolysin , 1992, Journal of bacteriology.

[10]  R. Drolet,et al.  Hemorrhagic gastroenteritis caused by Escherichia coli in piglets: Clinical, pathological and microbiological findings. , 1992, The Canadian veterinary journal = La revue veterinaire canadienne.

[11]  J. Kaper,et al.  Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector , 1991, Infection and immunity.

[12]  S. Opal,et al.  Aerobactin and alpha-hemolysin as virulence determinants in Escherichia coli isolated from human blood, urine, and stool. , 1990, The Journal of infectious diseases.

[13]  S. Tzipori,et al.  Nature and distribution of mucosal lesions associated with enteropathogenic and enterohemorrhagic Escherichia coli in piglets and the role of plasmid-mediated factors , 1989, Infection and immunity.

[14]  R. E. Rose,et al.  A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K [published erratum appears in Nucleic Acids Res 1988 Aug 11;16(15): 7762] , 1988, Nucleic Acids Res..

[15]  J. Mekalanos,et al.  A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR , 1988, Journal of bacteriology.

[16]  R. Welch,et al.  Transcriptional organization of the Escherichia coli hemolysin genes , 1988, Journal of bacteriology.

[17]  K. Kalland,et al.  Comparative study of colony hybridization with synthetic oligonucleotide probes and enzyme-linked immunosorbent assay for identification of enterotoxigenic Escherichia coli , 1988, Journal of clinical microbiology.

[18]  G. Bohach,et al.  [20] Purification of Escherichia coli α-hemolysin , 1988 .

[19]  J. Guillot,et al.  Cytotoxins in non-enterotoxigenic strains of Escherichia coli isolated from feces of diarrheic calves. , 1987, Veterinary microbiology.

[20]  Francis Dh,et al.  Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis. , 1986 .

[21]  D. Francis,et al.  Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis. , 1986, American journal of veterinary research.

[22]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[23]  Francis Dh.  Use of immunofluorescence, Gram's staining, histologic examination, and seroagglutination in the diagnosis of porcine colibacillosis. , 1983 .

[24]  S. Moseley,et al.  Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers , 1983, Infection and immunity.

[25]  M. Levine,et al.  Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines , 1983, Infection and immunity.

[26]  D. Francis.  Use of immunofluorescence, Gram's staining, histologic examination, and seroagglutination in the diagnosis of porcine colibacillosis. , 1983, American journal of veterinary research.

[27]  A. Pühler,et al.  A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria , 1983, Bio/Technology.

[28]  S. Falkow,et al.  Haemolysin contributes to virulence of extra-intestinal E. coli infections , 1981, Nature.

[29]  S. Falkow,et al.  Detection of enterotoxigenic Escherichia coli by DNA colony hybridization. , 1980, The Journal of infectious diseases.

[30]  T. Pál,et al.  alpha-Haemolysin: an additive virulence factor in Escherichia coli. , 1980, Acta microbiologica Academiae Scientiarum Hungaricae.

[31]  H. Murata,et al.  Relationship between the intestinal permeability to macromolecules and invasion of septicemia-inducing Escherichia coli in neonatal piglets , 1979, Infection and immunity.

[32]  S. Falkow,et al.  Cistrons encoding Escherichia coli heat-labile toxin , 1979, Journal of bacteriology.

[33]  O. P. Miniats,et al.  Gnotobiotic pigs-derivation and rearing. , 1978, Canadian journal of comparative medicine : Revue canadienne de medecine comparee.

[34]  R. C. Meyer,et al.  Experimental Peracute Colibacillosis: Gastrointestinal Lesions in Gnotobiotic Piglets , 1972, Veterinary pathology.

[35]  R. C. Meyer,et al.  Susceptibility of Gnotobiotic Swine to Escherichia coli Isolated from Nonenteric Human Infections , 1972 .

[36]  H. Smith,et al.  Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. , 1971, Journal of medical microbiology.

[37]  R. C. Meyer,et al.  Escherichia coli Isolated from Domestic Animals Pathogenic for Gnotobiotic Piglets , 1971, Infection and immunity.

[38]  O. P. Miniats,et al.  Response of gnotobiotic pigs to Escherichia coli. , 1970, Canadian journal of comparative medicine : Revue canadienne de medecine comparee.

[39]  L. Corley,et al.  Attachment and penetration of Escherichia coli into intestinal epithelium of the ileum in newborn pigs. , 1969, The American journal of pathology.

[40]  Switzer Wp,et al.  Application of the orbital sinus bleeding technique to swine. , 1969 .

[41]  G. Osweiler,et al.  Application of the orbital sinus bleeding technique to swine. , 1969, Laboratory animal care.

[42]  J. B. Spence,et al.  Escherichia coli Infection in Piglets , 1960 .

[43]  W. Sojka,et al.  Escherichia coli serotypes associated with certain pig diseases. , 1960 .