Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers

The first objective of this study was to evaluate the performance of fluorescence spectroscopy for diagnosing pre‐cancers in stratified squamous epithelial tissues in vivo using two different probe geometries with (1) overlapping versus (2) non‐overlapping illumination and collection areas on the tissue surface. Probe (1) and probe (2) are preferentially sensitive to the fluorescence originating from the tissue surface and sub‐surface tissue depths, respectively. The second objective was to design a novel, angled illumination fiber‐optic probe to maximally exploit the depth‐dependent fluorescence properties of epithelial tissues.

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