BIODISTRIBUTION AND STABILITY OF [ 65 ZN ] BLEOMYCIN COMPLEX

Bleomycin (BLM) has been labeled with various radioisotopes and widely used in therapy and diagnosis. In this study BLM was labeled with [Zn] zinc chloride and its distribution and stability in normal and tumor bearing mice was determined. The complex was obtained at the pH=2 in normal saline at 90 °C in 60 minutes. Radio-TLC showed an overall radiochemical yield of 95-97% (radiochemical purity >97%). The in vitro stability of the complex was determined in mice and human plasma. Preliminary studies were performed to determine distribution of [Zn]BLM in normal and tumor bearing mice on the basis of these results. [Zn]BLM may be used in therapeutic studies due to its suitable physico-chemical properties.

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