Growth and metabolism of mannitol by strains of Saccharomyces cerevisiae.

Of 40 polyploid strains of Saccharomyces cerevisiae screened for growth on D-mannitol (5%, w/v), half grew well (5-20 mg dry biomass ml-1). Certain of these strains were unable to grow on low concentrations of mannitol (1-2%, w/v) and others, initially unable to grow on mannitol, exhibited long-term adaptation to growth. An NAD+-dependent D-mannitol dehydrogenase (EC 1.1.1.67) was detected in mannitol-grown yeast. Growth was dependent on mitochondrial function and was obligately aerobic. Measurement of products of metabolism and respiratory activity indicated that growth on mannitol allows catabolite derepression.

[1]  A. Wiles.  IDENTIFICATION AND SIGNIFICANCE OF YEASTS ENCOUNTERED IN THE BREWERY , 1953 .

[2]  L. Bisson,et al.  Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[3]  A. Busturia,et al.  Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae. , 1986, Journal of general microbiology.

[4]  D. Lewis,et al.  CARBOHYDRATE PHYSIOLOGY OF MYCORRHIZAL ROOTS OF BEECH , 1965 .

[5]  N. Kaplan,et al.  HEXITOL METABOLISM IN ESCHERICHIA COLI , 1956, Journal of bacteriology.

[6]  E. Cabib,et al.  Regulation of glycogen synthesis in the intact yeast cell. , 1969, Biochemistry.

[7]  W. A. Maxwell,et al.  Mannitol Uptake by Saccharomyces cerevisiae , 1971 .

[8]  L. W. Parks,et al.  Metabolism of sterols in yeast. , 1978, CRC critical reviews in microbiology.

[9]  C. F. Strittmatter.  Adaptive variation in the level of oxidative activity in Saccharomyces cerevisiae. , 1957, Journal of general microbiology.

[10]  A. Linnane,et al.  Isolation of mitochondria and techniques for studying mitochondrial biogenesis in yeasts. , 1975, Methods in cell biology.

[11]  R. Doyle,et al.  Selective enhancement of glycolytic activity by incubation of yeast cells in sugars and polyols. , 1968, Canadian journal of microbiology.

[12]  M. Bard,et al.  Studies on the accumulation of ergosterol and 24(28)-dehydroergosterol in 3 strains of Saccharomyces cerevisiae. , 1974, Microbios.

[13]  A. Sols,et al.  Specificity of the constitutive hexose transport in yeast. , 1968, European journal of biochemistry.

[14]  D. Quain.  DIFFERENTIATION OF BREWING YEAST , 1986 .

[15]  O. Käppeli,et al.  Regulation of glucose metabolism in growing yeast cells. , 1981, Advances in microbial physiology.

[16]  R H De Deken,et al.  The Crabtree effect: a regulatory system in yeast. , 1966, Journal of general microbiology.

[17]  A. Linnane,et al.  THE BIOGENESIS OF MITOCHONDRIA , 1968, The Journal of cell biology.

[18]  P. Lord,et al.  Dependency of size of Saccharomyces cerevisiae cells on growth rate , 1979, Journal of bacteriology.

[19]  R. H. De Deken,et al.  The Crabtree Effect: A Regulatory System in Yeast , 1966 .

[20]  Johannes P. van Dijken,et al.  Redox balances in the metabolism of sugars by yeasts (NAD(H); NADP(H); glucose metabolism; xylose fermentation; ethanol; Crabtree effect; Custers effect) , 1986 .

[21]  G. Clark-Walker,et al.  THE BIOGENESIS OF MITOCHONDRIA IN SACCHAROMYCES CEREVISIAE , 1967, The Journal of cell biology.

[22]  E. Neufeld,et al.  CARBOHYDRATE METABOLISM. , 1965, Annual review of biochemistry.

[23]  B. Kirsop.  SEGREGATION OF STRAINS OF BREWING YEAST BY THEIR CARBON ASSIMILATION PATTERNS , 1974 .

[24]  J. A. Barnett.  The Catabolism of Acyclic Polyols by Yeasts , 1968 .

[25]  J. Haslam,et al.  The Effects of Catabolite Derepression on the Accumulation of Steryl Esters and the Activity of β-Hydroxymethylglutaryl-CoA Reductase in Saccharomyces cerevisiae , 1979 .

[26]  S. H. Lillie,et al.  Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation , 1980, Journal of bacteriology.

[27]  J. Haslam,et al.  The manipulation of cellular cytochrome and lipid composition in a haem mutant of Saccharomyces cerevisiae. , 1977, The Biochemical journal.

[28]  L. W. Parks,et al.  Acid-labilization of sterols for extraction from yeast. , 1977, Biochimica et biophysica acta.

[29]  J. Spencer,et al.  Genetic improvement of industrial yeasts. , 1983, Annual review of microbiology.

[30]  T. Bauchop,et al.  The growth of micro-organisms in relation to their energy supply. , 1960, Journal of general microbiology.

[31]  D. Quain.  THE DETERMINATION OF GLYCOGEN IN YEASTS , 1981 .

[32]  R. Lagunas.  Energy metabolism of Saccharomyces cerevisiae discrepancy between ATP balance and known metabolic functions. , 1976, Biochimica et biophysica acta.

[33]  J. Broach,et al.  The Molecular biology of the yeast Saccharomyces : metabolism and gene expression , 1982 .