This paper reports a series of laboratory and field measurements of spectral reflectance under artificial and natural light conditions which demonstrate that effects of natural chlorophyll fluorescence are observable in the reflectance red edge spectral region. These are results from the progress made to link physiologically-based indicators to optical indices from hyperspectral remote sensing in the Bioindicators of Forest Sustainability Project. This study is carried out on twelve sites of Acer saccharum M. in the Algoma Region, Ontario (Canada), where field measurements, laboratory-simulation experiments, and hyperspectral CASI imagery have been carried out in 1997, 1998, 1999 and 2000 campaigns. Leaf samples from the study sites have been used for reflectance and transmittance measurements with the Li-Cor Model 1800 integrating sphere apparatus coupled to an Ocean Optics Model ST1000 fibre spectrometer in which the same leaves are illuminated alternatively with and without fluorescence-exciting radiation. A study of the diurnal change in leaf reflectance spectra, combined with fluorescence measurements with the PAM-2000 Fluorometer show that the difference spectra are consistent with observed diurnal changes in steady-state fluorescence. Small canopies of Acer saccharum M. have been used for laboratory measurements with the CASI hyperspectral sensor, and under natural light conditions with a fibre spectrometer in diurnal trials, in which the variation of measured reflectance is shown experimentally to be consistent with a fluorescence signature imposed on the inherent leaf reflectance signature. Such reflectance changes due to CF are measurable under natural illumination conditions, although airborne experiments with the CASI hyperspectral sensor produced promising but less convincing results in two diurnal experiments carried out in 1999 and 2000, where small variations of reflectance due to the effect of CF were observed.
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