Expression of microRNA-27a in a rat model of osteonecrosis of the femoral head and its association with TGF-β/Smad7 signalling in osteoblasts

The present study assessed whether microRNA (miR)-27a is an influential factor in steroid-induced osteonecrosis of the femoral head (ONFH) and investigated the underlying mechanism of action. The results indicated that serum miR-27a was decreased in a rat model of ONFH compared with that in control rats. It was also observed that increased miR-27a expression promoted osteogenic differentiation and cell proliferation, inhibited caspase-3/9 and B-cell lymphoma-2-associated X protein expression and induced alkaline phosphatase (ALP) activity and bone morphogenetic protein (BMP)-2, runt-related transcription factor (Runx)2 and osteonectin mRNA expression in osteoblastic MC3T3-E1 cells. miR-27a mimics also induced transforming growth factor (TGF)-β and Smad7 protein expression in MC3T3-E1 cells. Furthermore, transfection with TGF-β expression plasmid was able to enhance the effects of miR-27a mimics on osteoblastic differentiation, cell proliferation, ALP activity, BMP-2, Runx2 and osteonectin mRNA expression, and Smad7 protein expression in the MC3T3-E1 cells. Transfection with a TGF-β or Smad7 expression plasmid also enhanced the effects of miR-27a mimics on osteoblastic differentiation, cell proliferation, ALP activity and osteonectin mRNA expression in the MC3T3-E1 cells. Taken together, the results of the present study suggested that the induction of TGF-β/Smad7 signaling in osteoblasts may be a potential mechanism by which miR-27a regulates steroid-induced ONFH.

[1]  Huafeng Wang,et al.  Arctigenin protects against liver injury from acute hepatitis by suppressing immune cells in mice. , 2018, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[2]  J. Ge,et al.  Liraglutide attenuates NLRP3 inflammasome-dependent pyroptosis via regulating SIRT1/NOX4/ROS pathway in H9c2 cells. , 2018, Biochemical and biophysical research communications.

[3]  Q. Lv,et al.  Norisoboldine, a natural AhR agonist, promotes Treg differentiation and attenuates colitis via targeting glycolysis and subsequent NAD+/SIRT1/SUV39H1/H3K9me3 signaling pathway , 2018, Cell Death & Disease.

[4]  M. Fujimuro,et al.  Arctigenin induces the apoptosis of primary effusion lymphoma cells under conditions of glucose deprivation. , 2017, International journal of oncology.

[5]  Sam L Cox,et al.  Processing of acceleration and dive data on‐board satellite relay tags to investigate diving and foraging behaviour in free‐ranging marine predators , 2017, Methods in ecology and evolution.

[6]  Zeyan Chen,et al.  MicroRNA-194 inhibition improves dietary-induced non-alcoholic fatty liver disease in mice through targeting on FXR. , 2017, Biochimica et biophysica acta. Molecular basis of disease.

[7]  Seong-ho Lee,et al.  Arctigenin represses TGF-β-induced epithelial mesenchymal transition in human lung cancer cells. , 2017, Biochemical and biophysical research communications.

[8]  F. Che,et al.  Arctigenin attenuates ischemic stroke via SIRT1-dependent inhibition of NLRP3 inflammasome. , 2017, Biochemical and biophysical research communications.

[9]  T. Standiford,et al.  Neutrophil transfer of miR-223 to lung epithelial cells dampens acute lung injury in mice , 2017, Science Translational Medicine.

[10]  G. Norata,et al.  Arctigenin improves vascular tone and decreases inflammation in human saphenous vein , 2017, European journal of pharmacology.

[11]  Xiaonan H. Wang,et al.  MicroRNA-23a and MicroRNA-27a Mimic Exercise by Ameliorating CKD-Induced Muscle Atrophy. , 2017, Journal of the American Society of Nephrology : JASN.

[12]  J. Baik,et al.  MIR‐27a regulates the TGF‐β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer , 2017, Molecular carcinogenesis.

[13]  Rui Chen,et al.  MicroRNA miR-23a cluster promotes osteocyte differentiation by regulating TGF-β signalling in osteoblasts , 2017, Nature Communications.

[14]  Xizeng Feng,et al.  Biodistribution of arctigenin-loaded nanoparticles designed for multimodal imaging , 2017, Journal of Nanobiotechnology.

[15]  Wei Zhang,et al.  Negative regulation of NLRP3 inflammasome by SIRT1 in vascular endothelial cells. , 2017, Immunobiology.

[16]  R. McCallum,et al.  Lubiprostone Accelerates Intestinal Transit and Alleviates Small Intestinal Bacterial Overgrowth in Patients With Chronic Constipation , 2016, The American journal of the medical sciences.

[17]  B. Sands,et al.  Assessing Colonic Exposure, Safety, and Clinical Activity of SRT2104, a Novel Oral SIRT1 Activator, in Patients with Mild to Moderate Ulcerative Colitis , 2015, Inflammatory bowel diseases.

[18]  Shan Sun,et al.  Mir-27a promotes apoptosis of cochlear sensory epithelium in Cx26 knockout mice. , 2016, Frontiers in bioscience.

[19]  Rui Li,et al.  miR-29a suppresses growth and metastasis in papillary thyroid carcinoma by targeting AKT3 , 2016, Tumor Biology.

[20]  Qiang Xu,et al.  Fumigaclavine C ameliorates dextran sulfate sodium-induced murine experimental colitis via NLRP3 inflammasome inhibition. , 2015, Journal of pharmacological sciences.

[21]  Yan Li,et al.  NF-κB signaling relieves negative regulation by miR-194 in hepatocellular carcinoma by suppressing the transcription factor HNF-1α , 2015, Science Signaling.

[22]  Xiaohuan Zou,et al.  MiRNA-194 Regulates Palmitic Acid-Induced Toll-Like Receptor 4 Inflammatory Responses in THP-1 Cells , 2015, Nutrients.

[23]  Y. Chen,et al.  Malondialdehyde and SOD-induced changes of gastric tissues in acute gastric mucosal injury under positive acceleration. , 2015, Genetics and molecular research : GMR.

[24]  Zhihong Wu,et al.  Preliminary screening of differentially expressed circulating microRNAs in patients with steroid‑induced osteonecrosis of the femoral head. , 2014, Molecular medicine reports.

[25]  J. Zhong,et al.  NLRP3 gene is associated with ulcerative colitis (UC), but not Crohn’s disease (CD), in Chinese Han population , 2014, Inflammation Research.

[26]  M. Lamkanfi,et al.  The Nlrp3 inflammasome: contributions to intestinal homeostasis. , 2011, Trends in immunology.

[27]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.