Molecular Identification of Korean Ginseng Cultivars (Panax ginseng C. A. Mey.) using Peptide Nucleic Acid (PNA) Microarray

This study was carried out to identify Korean ginseng cultivars using peptide nucleic acid (PNA) microarray. Sixty-seven probes were designed based on nucleotide variation to distinguish Korean ginseng cultivars of Panax ginseng. Among those PNA probes, three (PGB74, PGB110 and PGB130) have been developed to distinguish five Korean ginseng cultivars. Five Korean ginseng cultivars were denoted as barcode numbers depending on their fluorescent signal patterns of each cultivar using three probe sets in the PNA microarray. Five Korean ginseng cultivars, Chunpoong, Yunpoong, Gopo- ong, Gumpoong and Sunpoong, were simply denoted as '111', '222', '211', '221' and '122', respectively. This is the first report of PNA microarray which provided an objective and reliable method for the authentication of Korean ginseng culti- vars. Also, the PNA microarray will be useful for management system and pure guarantee in ginseng seed.

[1]  Young-Chang Kim,et al.  Analysis of Genetic Polymorphism of Korean Ginseng Cultivars and Foreign Accessions using SSR Markers , 2011 .

[2]  Young-Chang Kim,et al.  Development of SSR Markers for Identification of Korean Ginseng (Panax ginseng C. A. Meyer) Cultivars , 2011 .

[3]  R. Marchelli,et al.  Food analysis and food authentication by peptide nucleic acid (PNA)-based technologies. , 2011, Chemical Society reviews.

[4]  Young-Chang Kim,et al.  Development of peptide nucleic acid (PNA) microarray for identification of Panax species based on the nuclear ribosomal internal transcribed spacer (ITS) and 5.8S rDNA regions , 2010, Genes & Genomics.

[5]  Jeong-Woo Choi,et al.  Application of peptide nucleic acid towards development of nanobiosensor arrays. , 2010, Bioelectrochemistry.

[6]  Young-Chang Kim,et al.  Construction of genomic DNA library of Korean ginseng (Panax ginseng C. A. MEYER) and development of sequence-tagged sites. , 2010, Biological & pharmaceutical bulletin.

[7]  N. Ramchiary,et al.  Development and characterization of new microsatellite markers in Panaxginseng (C.A. Meyer) from BAC end sequences , 2010, Conservation Genetics.

[8]  J. Choi,et al.  Peptide Nucleic Acid-Based Array for Detecting and Genotyping Human Papillomaviruses , 2009, Journal of Clinical Microbiology.

[9]  S. Hamamah,et al.  Peptide nucleic acids (PNAs) as diagnostic devices for genetic and cytogenetic analysis. , 2008, Current pharmaceutical design.

[10]  Shiu-ying Hu.  The genusPanax (Ginseng) in Chinese medicine , 2008, Economic Botany.

[11]  Young Chang Kim,et al.  Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences , 2007, Plant Biotechnology Reports.

[12]  이은경,et al.  인삼(Panax ginseng C. A. Meyer)접합자 배의 형질전환을 위한 재분화 및 항생제 농도 조건 , 2003 .

[13]  B. Seong,et al.  Analysis of Diversity of Panax ginseng Collected in Korea by RAPD Technique , 2003 .

[14]  박현규,et al.  DNA Chip과 고정화 기술 , 2002 .

[15]  H. Chang,et al.  DNA Chip and Immobilization Technologies , 2002 .

[16]  Deok-Chun Yang,et al.  Comparison of ITS (Internal Transcribed Spacer) and 5.8S rDNA Sequences among Varieties and Cultivars in Panax ginseng , 2001 .

[17]  이명구,et al.  인삼 신품종 연풍의 육성경과 및 생육특성 ( Breeding Process and Characteristics of Yunpoong, a New Variety of Panax ginseng C.A. Meyer ) , 2000 .

[18]  P. Shaw,et al.  Molecular authentication of Panax species. , 1999, Phytochemistry.

[19]  이명구,et al.  인삼 우수계통 KG101의 육성경과 생육특성 ( Breeding Process and Characteristics of KG101 , a Superior Line of Panax ginseng C. A. Meyer ) , 1998 .

[20]  T. Namba,et al.  Application of PCR-RFLP and MASA analyses on 18S ribosomal RNA gene sequence for the identification of three Ginseng drugs. , 1997, Biological & pharmaceutical bulletin.

[21]  J. Wen,et al.  Phylogeny and biogeography of Panax L. (the ginseng genus, araliaceae): inferences from ITS sequences of nuclear ribosomal DNA. , 1996, Molecular phylogenetics and evolution.

[22]  P. Shaw,et al.  Authentication of Panax species and their adulterants by random-primed polymerase chain reaction. , 1995, Planta medica.

[23]  M. Egholm,et al.  Sequence-specific transcription arrest by peptide nucleic acid bound to the DNA template strand. , 1994, Gene.

[24]  임용표,et al.  Randomly Amplified Polymorphic DNA ( RAPD ) 기술을 이용한 고려인삼의 유전분석을 위한 Primer선발 및 변종별 비교 ( Survery of Proper Primers and Genetic Analysis of Korean Ginseng ( Panax ginseng C. A. Meyer ) Variants using the RAPD Technique ) , 1993 .

[25]  신희석,et al.  고려인삼의 화서 , 화뢰 , 과실 및 엽의 형태학적 특성 ( Morphological characteristics of inflorescence , flowering bud , fruit and leaf of Korean ginseng ) , 1982 .