A New Approach to Saffron Aroma

The aroma of saffron has received much attention from scientists in recent years, not only for the compounds that make it up, but also for its glycosidic precursors. Despite it all, the volatile generation mechanisms of the spice are almost completely unknown. Only the generation of safranal, the major compound, from picrocrocin has been established. The great effort carried out to detect and identify the volatile compounds of saffron has not been enougth to clarify which compounds are responsible for saffron aroma. In general, scientists has devoted little attention to the sample analyzed, taking for granted that all saffron is the same, something that makes it difficult to establish a comparison between the results obtained by the different authors, to the point that saffron aroma has not been defined yet. It must be clarified whether saffron aroma is what the consumer perceives via nasally when a container of the spice is uncovered, or whether on the contrary, it is the aroma conferred to food, normally after a thermal cooking process, and perceived retronasally. After an extensive bibliographic research, simple assays are suggested to understand what could be considered as saffron aroma, results that may help to delimit the research for future studies.

[1]  G. Alonso,et al.  Determination of Safranal from Saffron (Crocus sativus L.) by Thermal Desorption−Gas Chromatography , 1996 .

[2]  Eran Pichersky,et al.  The formation and function of plant volatiles: perfumes for pollinator attraction and defense. , 2002, Current opinion in plant biology.

[3]  P. Winterhalter,et al.  SAFFRON—RENEWED INTEREST IN AN ANCIENT SPICE , 2000 .

[4]  Y. Waché,et al.  Effect of cis/trans isomerism of beta-carotene on the ratios of volatile compounds produced during oxidative degradation. , 2003, Journal of agricultural and food chemistry.

[5]  M. R. Salinas,et al.  Adsorption-Thermal Desorption Gas Chromatography Applied to the Determination of Wine Aromas , 1994 .

[6]  A. Zalacain,et al.  Influence of the type of dehydration process on the sensory properties of saffron spice , 2002 .

[7]  J. Iborra,et al.  A non-destructive method to determine the safranal content of saffron (Crocus sativus L.) by supercritical carbon dioxide extraction combined with high-performance liquid chromatography and gas chromatography. , 2000, Journal of biochemical and biophysical methods.

[8]  F. Cormier,et al.  A highly specific glucosyltransferase is involved in the synthesis of crocetin glucosylesters in Crocus sativus cultured cells , 2001 .

[9]  M. Manfait,et al.  Separation of picrocrocin, cis-trans-crocins and safranal of saffron using high-performance liquid chromatography with photodiode-array detection. , 1994, Journal of chromatography. A.

[10]  A. H. Goliaris SAFFRON CULTIVATION IN GREECE , 1999 .

[11]  R. Buchecker,et al.  Absolute Konfiguration von Picrocrocin , 1973 .

[12]  L. Gómez-Gómez,et al.  Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas , 2004, Planta.

[13]  R. Kuhn,et al.  Über die Konstitution des Pikro‐crocins und seine Beziehung zu den Carotin‐Farbstoffen des Safrans , 1934 .

[14]  Moschos G. Polissiou,et al.  Isolation and Identification of the Aroma Components from Saffron (Crocus sativus) , 1997 .

[15]  Gian Luigi Rana,et al.  Volatile organic compounds from saffron , 2004 .

[16]  K. Sink,et al.  Development of an improved procedure for extraction and quantitation of safranal in stigmas of Crocus sativus L. using high performance liquid chromatography , 2000 .

[17]  Keith R Cadwallader,et al.  Freshness and shelf life of foods , 2002 .

[18]  M Carmona,et al.  Screening method for the detection of artificial colours in saffron using derivative UV-Vis spectrometry after precipitation of crocetin , 2005, Food additives and contaminants.

[19]  P. Variyar,et al.  Chemical investigation of gamma-irradiated saffron (Crocus sativus L.). , 2001, Journal of agricultural and food chemistry.

[20]  M. Désage,et al.  Identification and Isotopic Analysis of Safranal from Supercritical Fluid Extraction and Alcoholic Extracts of Saffron , 1996 .

[21]  H. Himeno,et al.  Synthesis of Crocin, Picrocrocin and Safranal by Saffron Stigma-like Structures Proliferated in Vitro , 1987 .

[22]  A. Zalacain,et al.  Influence of different drying and aging conditions on saffron constituents. , 2005, Journal of agricultural and food chemistry.

[23]  K. Cadwallader Flavor chemistry of saffron , 2002 .

[24]  D. Rajalakshmi,et al.  Saffron: Quality evaluation by sensory profile and gas chromatography. , 1992 .

[25]  M. Fernandez,et al.  Adsorption-Thermal Desorption-Gas Chromatography Applied to the Determination of Wine Aromas , 1997 .

[26]  G. Alonso,et al.  A NEW LOOK AT SAFFRON: MISTAKEN BELIEFS , 2004 .

[27]  N. Davies,et al.  Effect of drying temperature and air flow on the production and retention of secondary metabolites in saffron. , 2005, Journal of agricultural and food chemistry.

[28]  G. A. Ravishankar,et al.  Methods for the analysis of the saffron metabolites crocin, crocetins, picrocrocin and safranal for the determination of the quality of the spice using thin-layer chromatography, high-performance liquid chromatography and gas chromatography , 1992 .

[29]  M. Giaccio Crocetin from Saffron: An Active Component of an Ancient Spice , 2004, Critical reviews in food science and nutrition.

[30]  J. P. Yuan,et al.  Hydrolysis kinetics of astaxanthin esters and stability of astaxanthin of Haematococcus pluvialis during saponification. , 1999, Journal of agricultural and food chemistry.

[31]  P. Winterhalter,et al.  Novel Glycosidic Constituents from Saffron , 1997 .

[32]  P. Tarantilis,et al.  Qualitative determination of volatile compounds and quantitative evaluation of safranal and 4-hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde (HTCC) in Greek saffron. , 2004, Journal of agricultural and food chemistry.

[33]  E. Winterstein.,et al.  Uber Bestandteile des Safrans. I. Mitteilung. Über das Pikrocrocin , 1922 .

[34]  J. Mutterer,et al.  Oxidative remodeling of chromoplast carotenoids: identification of the carotenoid dioxygenase CsCCD and CsZCD genes involved in Crocus secondary metabolite biogenesis. , 2003, The Plant cell.

[35]  Frank David,et al.  Stir bar sorptive extraction (SBSE), a novel extraction technique for aqueous samples: Theory and principles† , 1999 .

[36]  E. Pichersky,et al.  Biochemical and molecular genetic aspects of floral scents. , 2000, Plant physiology.

[37]  M. Tsimidou,et al.  Saffron Quality: Effect of Agricultural Practices, Processing and Storage , 2004 .

[38]  S. G. Agarwal,et al.  Changes in Pigments and Volatiles of Saffron ( Crocus sativusL) During Processing and Storage , 1996 .

[39]  Wolfgang Rödel,et al.  Analysis of the volatile components of saffron , 1991 .

[40]  I. Singh,et al.  Analysis of Aroma Release from Scented Teas , 1997 .

[41]  R. Kuhn,et al.  Die Dihydroverbindung der isomeren Bixine und die Elektronen-Konfiguration der Polyene (Über konjugierte Doppelbindungen, XXIII. Mitteil.) , 1932 .

[42]  Peter Winterhalter,et al.  Identification of Novel Glycosidic Aroma Precursors in Saffron (Crocus sativus L.) , 1998 .

[43]  M. Sánchez-Fernández,et al.  Note. Safranal Content in Spanish Saffron , 2001 .

[44]  H. Baek,et al.  Characterization of Saffron Flavor by Aroma Extract Dilution Analysis , 1997 .

[45]  D. E. Heinz,et al.  Monoterpene aldehydes and isophorone-related compounds of saffron , 1971 .