Determination of α-chaconine and α-solanine in commercial potato crisps by QuEChERS extraction and UPLC-MS/MS

A simple and fast analytical method for the determination of the main steroidal glycoalkaloids, α-chaconine and α-solanine, in commercial potato crisps, based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction and ultra performance liquid chromatography coupled with an electrospray ionization triple-quadrupole tandem mass spectrometer (UPLC-MS/MS) in the multiple reaction monitoring mode was established and validated. The sample preparation procedure involves the extraction of the analytes with acidified acetonitrile and simultaneous liquid-liquid partitioning achieved by an addition of anhydrous magnesium sulfate and sodium acetate without any further clean-up steps. The limits of quantification (LOQs) for α-chaconine and α-solanine were 31 μg kg−1 and 16 μg kg−1 of fresh mass, respectively, at the signal-to-noise ratio (S/Ns) of 10. The method was applied in a survey of the content of α-chaconine and α-solanine in twenty commercial potato crisps from different brands. The results showed that all the products contained α-chaconine and α-solanine in widely varying concentrations. The amount of α-chaconine was higher than that of α-solanine in all samples.

[1]  Antonio V. Herrera-Herrera,et al.  Dispersive Solid‐Phase Extraction , 2015 .

[2]  J. Lachman,et al.  Effect of peeling and three cooking methods on the content of selected phytochemicals in potato tubers with various colour of flesh. , 2013, Food chemistry.

[3]  E. Primel,et al.  A method for the determination of multiclass pesticides in sugarcane juice employing QuEChERS and LC-ESI-MS/MS , 2013 .

[4]  S. Singh,et al.  Simple and reliable methods for the determination of three steroidal glycosides in the eight species of Solanum by reversed-phase HPLC coupled with diode array detection. , 2013, Phytochemical analysis : PCA.

[5]  I. Ferreira,et al.  Optimization and validation of a method based in a QuEChERS procedure and gas chromatography–mass spectrometry for the determination of multi-mycotoxins in popcorn , 2012 .

[6]  H. Ozier-Lafontaine,et al.  Insecticidal and antifungal chemicals produced by plants: a review , 2012, Environmental Chemistry Letters.

[7]  Roberto Romero-González,et al.  Development and validation of a multiclass method for the determination of veterinary drug residues in chicken by ultra high performance liquid chromatography-tandem mass spectrometry. , 2012, Talanta.

[8]  J. Švarc-Gajić,et al.  Development of a liquid chromatography-tandem mass spectrometric method for the simultaneous determination of tropane alkaloids and glycoalkaloids in crops , 2011, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[9]  Peter W. Jones,et al.  Bioactivities of glycoalkaloids and their aglycones from Solanum species. , 2011, Journal of agricultural and food chemistry.

[10]  R. Sheridan,et al.  Glycoalkaloid content in pet food by UPLC-tandem mass spectrometry. , 2010, Journal of chromatographic science.

[11]  Jinyong Peng,et al.  Simple and reliable methods for the determination of sixteen marker components for quality control of Daochi pill by HPLC coupled with diode array detection. , 2009, Phytochemical analysis : PCA.

[12]  O. Meyer,et al.  A 28-day repeat dose toxicity study of steroidal glycoalkaloids, alpha-solanine and alpha-chaconine in the Syrian Golden hamster. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[13]  M. Wink,et al.  Identification and Quantification of Steroidal Alkaloids from Wild Tuber-Bearing Solanum Species by HPLC and LC-ESI-MS , 2009, Potato Research.

[14]  Keshavan Niranjan,et al.  Potato glycoalkaloids: formation and strategies for mitigation , 2008 .

[15]  G. Lisińska,et al.  Changes in glycoalkaloids content of potatoes destined for consumption , 2008 .

[16]  M. Toledo,et al.  Effect of light and temperature on the formation of glycoalkaloids in potato tubers , 2007 .

[17]  G. Gołubowska,et al.  Changes of glycoalkaloids and nitrate contents in potatoes during chip processing , 2006 .

[18]  Roshani Shakya,et al.  Rapid screening of ascorbic acid, glycoalkaloids, and phenolics in potato using high-performance liquid chromatography. , 2006, Journal of agricultural and food chemistry.

[19]  Z. Polgar,et al.  Determination of Glycoalcaloids in Hungarian Potatoes by HPLC , 2006 .

[20]  G. Gołubowska,et al.  Changes in glycoalkaloid and nitrate contents in potatoes during French fries processing , 2005 .

[21]  J. Meulenbelt,et al.  Potato glycoalkaloids and adverse effects in humans: an ascending dose study. , 2005, Regulatory toxicology and pharmacology : RTP.

[22]  O. Fiehn,et al.  Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic field-grown potatoes. , 2005, Analytical biochemistry.

[23]  Steven J Lehotay,et al.  Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. , 2003, Journal of AOAC International.

[24]  P. Schmitt‐Kopplin,et al.  Evaluation of glycoalkaloids in tubers of genetically modified virus Y-resistant potato plants (var. Désirée) by non-aqueous capillary electrophoresis coupled with electrospray ionization mass spectrometry (NACE–ESI–MS) , 2003, Analytical and bioanalytical chemistry.

[25]  I. Vovk,et al.  High-performance thin-layer chromatographic determination of potato glycoalkaloids. , 2000, Journal of chromatography. A.

[26]  P. Hubert,et al.  Determination of alpha-solanine and alpha-chaconine in potatoes by high-performance thin-layer chromatography/densitometry. , 2000, Journal of AOAC International.

[27]  P. Sporns,et al.  A capillary electrophoresis laser-induced fluorescence method for analysis of potato glycoalkaloids based on a solution-phase immunoassay. 1. Separation and quantification of immunoassay products. , 2000, Journal of agricultural and food chemistry.

[28]  M. Friedman,et al.  Analysis of potato glycoalkaloids by a new ELISA kit , 1998 .

[29]  M. Friedman,et al.  Potato glycoalkaloids: chemistry, analysis, safety, and plant physiology , 1997 .

[30]  L. Plhak,et al.  Immunoassays for Toxic Potato Glycoalkaloids , 1996 .

[31]  David Smith,et al.  Potato glycoalkaloids: Some unanswered questions , 1996 .

[32]  K. Ng,et al.  Determination of Potato Glycoalkaloids Using Isotachophoresis and Comparison with a HPLC Method , 1994 .

[33]  K. Hellenäs,et al.  Determination of potato glycoalkaloids and their aglycone in blood serum by high-performance liquid chromatography. Application to pharmacokinetic studies in humans. , 1992, Journal of chromatography.

[34]  M. Leja,et al.  Changes in Total Phenolic, Total Glycoalkaloid, and Ascorbic Acid Content of Potatoes as a Result of Bruising , 1987 .

[35]  F. Shahidi,et al.  Control of lipid oxidation in cooked meats by combinations of antioxidants and chelators , 1987 .

[36]  R. Bushway,et al.  .alpha.-Chaconine and .alpha.-solanine content of potato products and their stability during several modes of cooking , 1981 .

[37]  L. E. Spencer,et al.  Acetaldehyde as an end-point indicator in the catalytic thermometric titration of weak acids. , 1977, Talanta.