Effects of Arum dioscoridis Extract on Hepatic Toxicity Caused by Thioacetamide in Rats

Background: The aim of this study was to investigate the prophylactic and therapeutic effects of Arum dioscoridis (tirsik) plant extract against thioacetamide-induced experimental liver toxicity. Method s: In this study, 35 male Wistar–Albino rats, of 12-14 weeks old, weighing between 200 and 270 g, were used. Rats were divided into 5 groups of 7 each. The first group was determined as the control group, the second group as the hepatotoxicity group, the third group as the prophylaxis group, the fourth group as the intraperitoneal treatment group, and the fifth group as the oral treatment group. Hepatotoxicity was achieved with a single intraperitoneal dose of 350 mg/kg of thioacetamide (TAA). On the seventh day, the rats were sacrificed under general anesthesia. Their blood was taken and liver enzymes were studied. Malondialdehyde (MDA), glutathyon peroxidase (GPx), catalase (CAT), superoxit dismutase (SOD) enzymes were studied from liver tissues. In addition, liver tissues were evaluated histopathologically. Results: With Arum dioscoridis treatment and prophylaxis, improvements in all parameters and increases in tissue antioxidant levels were detected. Conclusion: It was determined that Arum dioscoridis plant extract has prophylactic and therapeutic effects on liver toxicity. In cases of acute liver injury and hepatotoxicity, we suggest the potential application of Arum dioscoridis for effective and inexpensive treatment.

[1]  F. Koçer,et al.  Antimicrobial activities of some bryophytes in Turkey , 2018 .

[2]  N. Sekeroglu,et al.  Total phenolic content, ferric reducing and DPPH scavenging activity of Arum dioscoridis , 2015, Natural product research.

[3]  C. Loddenkemper,et al.  A guide to histomorphological evaluation of intestinal inflammation in mouse models. , 2014, International journal of clinical and experimental pathology.

[4]  O. M. A. Abdel Salam,et al.  The effect of antidepressant drugs on thioacetamide-induced oxidative stress. , 2013, European review for medical and pharmacological sciences.

[5]  S. Yildiz,et al.  SCREENING OF PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF ARUM DIOSCORIDIS SEEDS , 2012 .

[6]  J. O'connor,et al.  Mitochondrial dysfunction, persistent oxidative damage, and catalase inhibition in immune cells of naïve and treated Crohn's disease , 2010, Inflammatory bowel diseases.

[7]  P. Reddanna,et al.  Fulminant hepatic failure induced oxidative stress in nonsynaptic mitochondria of cerebral cortex in rats , 2004, Neuroscience Letters.

[8]  A. Burke,et al.  Non‐Alcoholic Fatty Liver Disease, Non‐Alcoholic Steatohepatitis and Orthotopic Liver Transplantation , 2004, American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons.

[9]  M. Cooke,et al.  Factors contributing to the outcome of oxidative damage to nucleic acids , 2004, BioEssays : news and reviews in molecular, cellular and developmental biology.

[10]  K. Nakahara,et al.  Antibacterial activity of extracts from some edible plants commonly consumed in Asia. , 2003, International journal of food microbiology.

[11]  I. Benjamin,et al.  Reproducible production of thioacetamide-induced macronodular cirrhosis in the rat with no mortality. , 2002, Journal of hepatology.

[12]  H. Dashti,et al.  Level of Superoxide Dismutase, Glutathione Peroxidase and Uric Acid in Thioacetamide‐Induced Cirrhotic Rats , 2002, Anatomia, histologia, embryologia.

[13]  S. Yasar,et al.  In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats , 2001, Archives of Toxicology.

[14]  P. Vuorela,et al.  Effects of simple aromatic compounds and flavonoids on Ca2+ fluxes in rat pituitary GH(4)C(1) cells. , 2001, European journal of pharmacology.

[15]  J. Matés,et al.  Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. , 2000, Toxicology.

[16]  H. Chung,et al.  Expression of genes coding for antioxidant enzymes and heat shock proteins is altered in primary cultures of rat hepatocytes , 1996, Journal of cellular physiology.

[17]  X. Leng,et al.  Changes of blood humoral substances in experimental cirrhosis and their effects on portal hemodynamics. , 1990, Chinese medical journal.

[18]  R. Floyd.  The role of 8-hydroxyguanine in carcinogenesis. , 1990, Carcinogenesis.

[19]  R. Barneby,et al.  Flora of Turkey and the East Aegean Islands. , 1966 .

[20]  M. Ryska,et al.  Acute liver failure induced by thioacetamide: selection of optimal dosage in Wistar and Lewis rats. , 2014, Physiological research.

[21]  K. S. Dayisoylu.  Changes of antioxidant activity in different forms and meal of Arum maculatum in Kahramanmaras province from Turkey. , 2010 .

[22]  K. Frenkel.  Carcinogen-mediated oxidant formation and oxidative DNA damage. , 1992, Pharmacology & therapeutics.