An integrated microfluidic system for bovine DNA purification and digital PCR detection.

In this paper, we described an integrated modularized microfluidic system that contained two distinct functional modules, one for nucleic acids (NA) extraction and the other for digital PCR (dPCR), allowing for detecting the bovine DNA in ovine tissue.

[1]  Wei Jin,et al.  Integrated glass microdevice for nucleic acid purification, loop-mediated isothermal amplification, and online detection. , 2011, Analytical chemistry.

[2]  Andreas Manz,et al.  Latest developments in micro total analysis systems. , 2010, Analytical chemistry.

[3]  Jiashu Sun,et al.  Integrated microcapillary for sample-to-answer nucleic acid pretreatment, amplification, and detection. , 2014, Analytical chemistry.

[4]  Yusi Fu,et al.  Digital polymerase chain reaction in an array of femtoliter polydimethylsiloxane microreactors. , 2012, Analytical chemistry.

[5]  Bruce K Gale,et al.  Spinning disk platform for microfluidic digital polymerase chain reaction. , 2010, Analytical chemistry.

[6]  Anupam Singhal,et al.  Megapixel digital PCR , 2011, Nature Methods.

[7]  K. A. Wolfe,et al.  Toward a microchip‐based solid‐phase extraction method for isolation of nucleic acids , 2002, Electrophoresis.

[8]  Jerome P Ferrance,et al.  An automated micro-solid phase extraction device involving integrated \high-pressure microvalves for genetic sample preparation , 2009, Biomedical microdevices.

[9]  James P Landers,et al.  Microchip-based solid-phase purification of RNA from biological samples. , 2008, Analytical chemistry.

[10]  Bingwen Yu,et al.  A localized temporary negative pressure assisted microfluidic device for detecting keratin 19 in A549 lung carcinoma cells with digital PCR , 2015 .

[11]  C. Batt,et al.  Nucleic acid purification using microfabricated silicon structures. , 2003, Biosensors & bioelectronics.

[12]  Bingcheng Lin,et al.  Fabrication of two‐weir structure‐based packed columns for on‐chip solid‐phase extraction of DNA , 2007, Electrophoresis.

[13]  Kelvin J. Liu,et al.  A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification. , 2011, Lab on a chip.