A low-cost, low-power consumption, miniature laser-induced fluorescence system for DNA detection on a microfluidic device.

With a focus on low-cost and low-power consumption, a miniature laser-induced fluorescence (LIF) detection system was assembled using a 635 nm red diode laser as the excitation source and a photodiode element coupled with an operational amplifier for signal collection. The primary elements of the miniature system, namely the laser and the detection system, cost a combined $70 and required only 270 mW of power for operation. When compared to conventional systems assembled using an argon-ion laser source and a photomultiplier tube, this represents a 98% decrease in the cost, and greater than 5000-fold decrease in power consumption. Quantitation of DNA on microdevices using the miniature LIF detection system was also performed with an error of less than 15%. This detection system is a step in the direction of commercializing microfluidic instrumentation by reducing the cost and power required for operation.

[1]  J P Landers,et al.  Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds. , 2001, Analytical biochemistry.

[2]  Karel Klepárník,et al.  Detection of DNA fragmentation in a single apoptotic cardiomyocyte by electrophoresis on a microfluidic device , 2003, Electrophoresis.

[3]  B. Legendre,et al.  Ultrasensitive near-infrared laser-induced fluorescence detection in capillary electrophoresis using a diode laser and avalanche photodiode. , 1997, Journal of chromatography. A.

[4]  J P Landers,et al.  Molecular diagnostics on microfabricated electrophoretic devices: from slab gel- to capillary- to microchip-based assays for T- and B-cell lymphoproliferative disorders. , 1999, Clinical chemistry.

[5]  R. Mathies,et al.  Single-molecule detection of DNA separations in microfabricated capillary electrophoresis chips employing focused molecular streams. , 1999, Analytical chemistry.

[6]  A D Stroock,et al.  An integrated fluorescence detection system in poly(dimethylsiloxane) for microfluidic applications. , 2001, Analytical chemistry.

[7]  E. Yeung,et al.  Diode laser-induced fluorescence detection in capillary electrophoresis after pre-column derivatization of amino acids and small peptides. , 1995, Journal of chromatography. A.

[8]  Dimitra N. Stratis-Cullum,et al.  A miniature biochip system for detection of aerosolized Bacillus globigii spores. , 2003, Analytical chemistry.

[9]  C. Culbertson,et al.  Microchip Structures for Submillisecond Electrophoresis , 1998 .