The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.

Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most serious diseases of rice. Here we describe the isolation and characterization of Pib, one of the rice blast resistance genes. The Pib gene was isolated by a map-based cloning strategy. The deduced amino acid sequence of the Pib gene product contains a nucleotide binding site (NBS) and leucine-rich repeats (LRRs); thus, Pib is a member of the NBS-LRR class of plant disease resistance genes. Interestingly, a duplication of the kinase 1a, 2 and 3a motifs of the NBS region was found in the N-terminal half of the Pib protein. In addition, eight cysteine residues are clustered in the middle of the LRRs, a feature which has not been reported for other R genes. Pib gene expression was induced upon altered environmental conditions, such as altered temperatures and darkness.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  R. Michelmore,et al.  The Major Resistance Gene Cluster in Lettuce Is Highly Duplicated and Spans Several Megabases , 1998, Plant Cell.

[3]  J. Mes,et al.  Dissection of the Fusarium I2 Gene Cluster in Tomato Reveals Six Homologs and One Active Gene Copy , 1998, Plant Cell.

[4]  M. Yano,et al.  Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[5]  S. Dinesh-Kumar,et al.  Signaling in plant-microbe interactions. , 1997, Science.

[6]  E. Finnegan,et al.  Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region. , 1997, The Plant cell.

[7]  M. Yano,et al.  Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b , 1997, Theoretical and Applied Genetics.

[8]  Daniel T. Lavelle,et al.  Tomato Prf Is a Member of the Leucine-Rich Repeat Class of Plant Disease Resistance Genes and Lies Embedded within the Pto Kinase Gene Cluster , 1996, Cell.

[9]  Li-li Chen,et al.  A Receptor Kinase-Like Protein Encoded by the Rice Disease Resistance Gene, Xa21 , 1995, Science.

[10]  S. Kang,et al.  The PWL host specificity gene family in the blast fungus Magnaporthe grisea. , 1995, Molecular plant-microbe interactions : MPMI.

[11]  J. Dangl,et al.  Structure of the Arabidopsis RPM1 gene enabling dual specificity disease resistance , 1995, Science.

[12]  F. Chumley,et al.  Identification, cloning, and characterization of PWL2, a gene for host species specificity in the rice blast fungus. , 1995, The Plant cell.

[13]  E. Finnegan,et al.  The L6 gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N. , 1995, The Plant cell.

[14]  G. Martin,et al.  Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes. , 1995, Trends in genetics : TIG.

[15]  S. Lin,et al.  A 300 kilobase interval genetic map of rice including 883 expressed sequences , 1994, Nature Genetics.

[16]  F. Ausubel,et al.  The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats , 1994, Cell.

[17]  S. Dinesh-Kumar,et al.  The product of the tobacco mosaic virus resistance gene N: Similarity to toll and the interleukin-1 receptor , 1994, Cell.

[18]  J. Giraudat,et al.  RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. , 1994, Science.

[19]  R. Nelson,et al.  RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. , 1994, Genetics.

[20]  P. Quail,et al.  Expression of a Maize Ubiquitin Gene Promoter-bar Chimeric Gene in Transgenic Rice Plants. , 1992, Plant physiology.

[21]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[22]  S. Kiyosawa.  Genetics and Epidemiological Modeling of Breakdown of Plant Disease Resistance , 1982 .

[23]  W. F. Thompson,et al.  Rapid isolation of high molecular weight plant DNA. , 1980, Nucleic acids research.

[24]  H H Flor,et al.  Current Status of the Gene-For-Gene Concept , 1971 .

[25]  S. Lin,et al.  A high-density rice genetic linkage map with 2275 markers using a single F2 population. , 1998, Genetics.

[26]  Jonathan D. G. Jones,et al.  The Role of Leucine-Rich Repeat Proteins in Plant Defences , 1997 .

[27]  S. Kawasaki,et al.  High resolution mapping of the Indica-derived rice blast reisistance genes. I. Pi-b , 1996 .

[28]  K. Shimamoto,et al.  Electroporation-Mediated Gene Transfer to Japonica Rice Protoplasts and Regeneration of Transgenic Plants , 1995 .

[29]  S. Datta.  Polyethylene-Glycol-Mediated Direct Gene Transfer to Indica Rice Protoplasts and Regeneration of Transgenic Plants , 1995 .

[30]  T. Kinoshita.  Report of committee on gene symbolization, nomenclature and linkage groups , 1995 .

[31]  G. Churchill,et al.  Pooled-sampling makes high-resolution mapping practical with DNA markers. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[32]  D. Mackill,et al.  Inheritance of Blast Resistance in Near-Isogenic Lines of Rice , 1992 .

[33]  D. D. Kosambi.  The estimation of map distances from recombination values. , 1943 .