Comparative evaluation of the efficacy of commercial and prototype PRRS subunit vaccines against an HP-PRRSV challenge

The objective of this study was to compare the efficacy of a commercial porcine reproductive and respiratory syndrome (PRRS) subunit vaccine and a prototype PRRS II subunit vaccine against a highly pathogenic PRRS virus (HP-PRRSV) in pigs. Both vaccines were administered intramuscularly in 2 doses at 21 and 42 days of age, and the pigs were challenged intranasally with HP-PRRSV at 63 days of age. Pigs vaccinated with the prototype PRRS II subunit vaccine had significantly higher anti-PRRSV antibody titers, a greater number of interferon-γ-secreting cells, and a greater reduction in lung lesion scores compared to pigs vaccinated with the commercial PRRS subunit vaccine. Therefore, the commercial PRRS subunit and prototype PRRS II subunit vaccines are efficacious against HP-PRRSV.

[1]  C. Chae,et al.  Evaluation of the new commercial recombinant chimeric subunit vaccine PRRSFREE in challenge with heterologous types 1 and 2 porcine reproductive and respiratory syndrome virus. , 2017, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.

[2]  A. Zakhartchouk,et al.  Anti-apoptosis in porcine respiratory and reproductive syndrome virus , 2016, Virulence.

[3]  C. Chae,et al.  Comparison of two genetically distant type 2 porcine reproductive and respiratory syndrome virus (PRRSV) modified live vaccines against Vietnamese highly pathogenic PRRSV. , 2015, Veterinary microbiology.

[4]  J. Bates,et al.  Pigs immunized with Chinese highly pathogenic PRRS virus modified live vaccine are protected from challenge with North American PRRSV strain NADC-20. , 2015, Vaccine.

[5]  T. T. Nguyen,et al.  Comparison of experimental infection with northern and southern Vietnamese strains of highly pathogenic porcine reproductive and respiratory syndrome virus. , 2015, Journal of comparative pathology.

[6]  K. Lager,et al.  Efficacy of Type 2 PRRSV vaccine against Chinese and Vietnamese HP-PRRSV challenge in pigs. , 2014, Vaccine.

[7]  C. Chae,et al.  Evaluation of the efficacy of a new modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine (Fostera PRRS) against heterologous PRRSV challenge. , 2014, Veterinary microbiology.

[8]  W. Feng,et al.  Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice , 2014, Journal of veterinary science.

[9]  Zuzhang Wei,et al.  Immunization of pigs with a type 2 modified live PRRSV vaccine prevents the development of a deadly long lasting hyperpyrexia in a challenge study with highly pathogenic PRRSV JX143. , 2013, Vaccine.

[10]  B. Lee,et al.  Evaluation of monoclonal antibody–based immunohistochemistry for the detection of European and North American Porcine reproductive and respiratory syndrome virus and a comparison with in situ hybridization and reverse transcription polymerase chain reaction , 2012, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[11]  K. Tian,et al.  Emergence and pathogenicity of highly pathogenic Porcine reproductive and respiratory syndrome virus in Vientiane, Lao People’s Democratic Republic , 2012, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[12]  G. Gao,et al.  Porcine Respiratory and Reproductive Syndrome Virus Variants, Vietnam and China, 2007 , 2008, Emerging infectious diseases.

[13]  F. Osorio,et al.  Assessment of the efficacy of commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines based on measurement of serologic response, frequency of gamma-IFN-producing cells and virological parameters of protection upon challenge. , 2007, Veterinary microbiology.

[14]  Di Liu,et al.  Emergence of Fatal PRRSV Variants: Unparalleled Outbreaks of Atypical PRRS in China and Molecular Dissection of the Unique Hallmark , 2007, PloS one.

[15]  F. Joisel,et al.  Porcine reproductive and respiratory syndrome (PRRS) virus-specific interferon-gamma(+) T-cell responses after PRRS virus infection or vaccination with an inactivated PRRS vaccine. , 2005, Viral immunology.

[16]  I. Díaz,et al.  Use of ELISPOT and ELISA to evaluate IFN-γ, IL-10 and IL-4 responses in conventional pigs , 2005 .

[17]  I. Díaz,et al.  Use of ELISPOT and ELISA to evaluate IFN-gamma, IL-10 and IL-4 responses in conventional pigs. , 2005, Veterinary immunology and immunopathology.

[18]  M. Torremorell,et al.  Detection of U.S., Lelystad, and European-Like Porcine Reproductive and Respiratory Syndrome Viruses and Relative Quantitation in Boar Semen and Serum Samples by Real-Time PCR , 2004, Journal of Clinical Microbiology.

[19]  F. Osorio,et al.  Gradual development of the interferon-gamma response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination. , 2003, Virology.

[20]  R. Forsberg,et al.  Reversion of a live porcine reproductive and respiratory syndrome virus vaccine investigated by parallel mutations. , 2001, The Journal of general virology.

[21]  Robert H. Schmidt,et al.  2000 Report of the AVMA Panel on Euthanasia. , 2001, Journal of the American Veterinary Medical Association.

[22]  P. Halbur,et al.  Comparison of the Antigen Distribution of Two US Porcine Reproductive and Respiratory Syndrome Virus Isolates with that of the Lelystad Virus , 1996 .

[23]  P. Halbur,et al.  Comparison of the Pathogenicity of Two US Porcine Reproductive and Respiratory Syndrome Virus Isolates with that of the Lelystad Virus , 1995, Veterinary pathology.