Running head (max. 50 characters): Systems genetics of fatty acids in Brassica rapa

1 Title: A systems genetics approach identifies gene regulatory networks associated with 2 fatty acid composition in Brassica rapa seed 3 Authors: 4 Ram Kumar Basnet, Dunia Pino Del Carpio, Dong Xiao, Johan Bucher, Mina Jin, 5 Kerry Boyle, Pierre Fobert, Richard G. F. Visser, Chris Maliepaard, Guusje 6 Bonnema 7 8 Laboratory of Plant Breeding, Wageningen University, Wageningen, The Netherlands, 9 Centre for BioSystems Genomics, Wageningen, The Netherlands, 10 Current address: Department of Plant Breeding and Genetics, Cornell University, Ithaca, 11 New York, USA, 12 Current address: State Key Laboratory of Crop Genetics and Germplasm Enhancement, 13 Horticultural College, Nanjing Agricultural University, Nanjing, China 14 Department of Agricultural Biotechnology, National Academy of Agricultural Science, 15 Rural Development Administration, 150 Suin-ro, Gwonseon-gu, Suwon 441-707, Korea 16 Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Canada 17 Corresponding author name: Guusje Bonnema 18 Address: Wageningen UR Plant Breeding, 19 Wageningen University, 20 Droevendaalsesteeg 1 (Radix building 107; room 2.166) 21 6708 PB Wageningen, 22 The Netherlands. 23 Tel: +31 317 484028 24 E-mail: Guusje.Bonnema@wur.nl 25 The journal research area most appropriate for the paper: Genetics, Genomics, and Systems 26 biology. 27 The authors responsible for distribution of materials integral to the findings presented in this 28 article in accordance with the policy described in the Instructions for Authors 29 (www.plantphysiol.org) is: Guusje Bonnema (Guusje.Bonnema@wur.nl). 30 Author contributions 31 RKB designed and conducted the experiments, collected samples, analyzed data, performed 32 network construction and wrote the manuscript. DPDC was involved in marker 33 development and reviewed the manuscript, DX was involved in the marker development 34 and performed RT-qPCR experiment, JB provided technical support, MJ was involved in 35 marker development, KB did fatty acid measurement, PF was involved in fatty acid 36 measurement, RGFV reviewed the manuscript, CM and GB designed and supervised the 37 Plant Physiology Preview. Published on October 30, 2015, as DOI:10.1104/pp.15.00853

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