Coulometric microdevice for organophosphate pesticide detection

Abstract A coulometric microdevice based on plug-based microfluidics was developed for the detection of organophosphate pesticides (OPs). Detection was based on the inhibition of an enzyme, acetylcholinesterase (AChE), by the OPs. Hydrogen peroxide (H2O2), produced in a series of enzymatic reactions involving AChE and choline oxidase (ChOx), was oxidized on a microelectrode array, and changes in AChE activity upon addition of an OP were measured by coulometry. A linear relationship was confirmed between the charge generated and the logarithm of the OP concentration. The lower limit of detection (LOD) was 33 nM for malathion and 90 nM for acephate, MEP, and diazinon. An increase in the ratio of organic solvents was also found to inhibit AChE activity. However, the concentrations sufficient to produce this effect were several orders of magnitude higher than those of the OPs, indicating that this influence can be ignored in the analysis of OPs.

[1]  Charlotte Carlsson,et al.  Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases , 2005 .

[2]  Yuehe Lin,et al.  Carbon nanotube-based electrochemical sensor for assay of salivary cholinesterase enzyme activity: an exposure biomarker of organophosphate pesticides and nerve agents. , 2008, Environmental science & technology.

[3]  Andreas Manz,et al.  Latest developments in micro total analysis systems. , 2010, Analytical chemistry.

[4]  M. Castaño‐Álvarez,et al.  Smart portable electrophoresis instrument based on multipurpose microfluidic chips with electrochemical detection , 2012, Electrophoresis.

[5]  A. Avramescu,et al.  Detection of organophosphorus insecticides with immobilized acetylcholinesterase – comparative study of two enzyme sensors , 2002, Analytical and bioanalytical chemistry.

[6]  Hiroaki Suzuki,et al.  Thin-Film Ag/AgCl Structure and Operational Modes to Realize Long-Term Storage , 2001 .

[7]  Joseph Wang,et al.  Microchip devices for detecting terrorist weapons , 2003 .

[8]  J. Hart,et al.  A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides. , 2011, Biosensors & bioelectronics.

[9]  A Mulchandani,et al.  Flow injection amperometric enzyme biosensor for direct determination of organophosphate nerve agents. , 2001, Environmental science & technology.

[10]  Shengyang Tao,et al.  Ultra-sensitive biosensor based on mesocellular silica foam for organophosphorous pesticide detection. , 2011, Biosensors & bioelectronics.

[11]  J. Marty,et al.  Flow-injection amperometric determination of pesticides on the basis of their inhibition of immobilized acetylcholinesterases of different origin , 2002, Analytical and bioanalytical chemistry.

[12]  Andreas Manz,et al.  Microfluidics: Applications for analytical purposes in chemistry and biochemistry , 2008, Electrophoresis.

[13]  M. Pumera,et al.  Lab-on-a-chip for ultrasensitive detection of carbofuran by enzymatic inhibition with replacement of enzyme using magnetic beads. , 2009, Lab on a chip.

[14]  J. Landers,et al.  Development of a micro-total analysis system (μ-TAS) for the determination of catecholamines , 2010, Analytical and bioanalytical chemistry.

[15]  P. D Patel,et al.  (Bio)sensors for measurement of analytes implicated in food safety: a review , 2002 .

[16]  Fumihiro Sassa,et al.  Coulometric detection of components in liquid plugs by microfabricated flow channel and electrode structures. , 2010, Analytical chemistry.

[17]  Jean-Louis Marty,et al.  Twenty years research in cholinesterase biosensors: from basic research to practical applications. , 2006, Biomolecular engineering.

[18]  Plamen Atanasov,et al.  Direct electron transfer based tri-enzyme electrode for monitoring of organophosphorus pesticides , 1996 .

[19]  Joseph Wang,et al.  Disposable Carbon Nanotube Modified Screen‐Printed Biosensor for Amperometric Detection of Organophosphorus Pesticides and Nerve Agents , 2004 .

[20]  Helen Song,et al.  Reactions in droplets in microfluidic channels. , 2006, Angewandte Chemie.

[21]  Jean-Louis Marty,et al.  Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides , 2002 .

[22]  A. Ciucu,et al.  Detection of pesticides using an amperometric biosensor based on ferophthalocyanine chemically modified carbon paste electrode and immobilized bienzymatic system. , 2003, Biosensors & bioelectronics.

[23]  A. Manz,et al.  Micro total analysis systems. Latest advancements and trends. , 2006, Analytical chemistry.

[24]  Fumihiro Sassa,et al.  Microprocessing of liquid plugs for bio/chemical analyses. , 2008, Analytical chemistry.