Large-Scale Cell Culture Technology

Publisher Summary Large-scale growth of vertebrate cells in vitro offers a method for the production of biomolecules not available from other sources. This has focused the attention of researchers on the development of mass culture systems that offer opportunities for large-scale growth of such cells for production of desired biomolecules. This chapter describes a variety of mass culture systems that can be operated antibiotic-free and can be selected to meet the specific requirements for each cell/product combination. It reviews the data on the vertebrate culture methods currently available and in use for the production of biologically important molecules with a particular focus on large-scale cell culture technology. The chapter presents a review of present large-scale cell culture technology and describes the development in the laboratory of significant advances in a new, multifaceted approach to this area. It would be useful both for the establishment of large-scale cell culture facilities dedicated to the production of biomolecules for clinical or commercial application and for the research laboratory.

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