The effect of protein-coated contact lenses on the adhesion and viability of gram negative bacteria

Purpose. Gram negative bacterial adhesion to contact lenses can cause adverse responses. During contact lens wear, components of the tear film adsorb to the contact lens. This study aimed to investigate the effect of this conditioning film on the viability of bacteria. Methods. Bacteria adhered to contact lenses which were either unworn, worn for daily-, extended- or overnight-wear or coated with lactoferrin or lysozyme. Numbers of viable and total cells were estimated. Results. The number of viable attached cells was found to be significantly lower than the total number of cells on worn (50% for strain Paer1 on daily-wear lenses) or lactoferrin-coated lenses (56% for strain Paer1). Lysozyme-coated lenses no statistically significant effect on adhesion. Discussion. The conditioning film gained through wear may not inhibit bacterial adhesion, but may act adversely upon those bacteria that succeed in attaching.

[1]  K. Juárez,et al.  The Pseudomonas aeruginosa rhlAB Operon Is Not Expressed during the Logarithmic Phase of Growth Even in the Presence of Its Activator RhlR and the Autoinducer N-Butyryl-Homoserine Lactone , 2003, Journal of bacteriology.

[2]  H. C. van der Mei,et al.  Influence of wear and overwear on surface properties of etafilcon A contact lenses and adhesion of Pseudomonas aeruginosa. , 2002, Investigative ophthalmology & visual science.

[3]  C. Keel,et al.  Genetically programmed autoinducer destruction reduces virulence gene expression and swarming motility in Pseudomonas aeruginosa PAO1. , 2002, Microbiology.

[4]  H. J. Griesser,et al.  XPS and surface-MALDI-MS characterisation of worn HEMA-based contact lenses. , 2001, Biomaterials.

[5]  H. C. van der Mei,et al.  Bacterial adhesion to surface hydrophilic and hydrophobic contact lenses. , 2001, Biomaterials.

[6]  D. Ammon,et al.  The relationship between contact lens surface charge and in-vitro protein deposition levels. , 2001, Biomaterials.

[7]  B. Bassler,et al.  Quorum sensing in bacteria. , 2001, Annual review of microbiology.

[8]  M. Willcox,et al.  Association of Acinetobacter Species With Contact Lens–induced Adverse Responses , 2001, Cornea.

[9]  Savitri Sharma,et al.  Bacterial Colonization of Disposable Soft Contact Lenses Is Greater during Corneal Infiltrative Events than during Asymptomatic Extended Lens Wear , 2000, Journal of Clinical Microbiology.

[10]  H. Ostolaza,et al.  Permeabilizing action of an antimicrobial lactoferricin‐derived peptide on bacterial and artificial membranes , 1999, FEBS letters.

[11]  E. Hume,et al.  Adhesion and growth of Serratia marcescens on artificial closed eye tears soaked hydrogel contact lenses. , 1997, Australian and New Zealand journal of ophthalmology.

[12]  Savitri Sharma,et al.  Haemophilus influenzae adherent to contact lenses associated with production of acute ocular inflammation , 1996, Journal of clinical microbiology.

[13]  G. Pier,et al.  Relationship between cytotoxicity and corneal epithelial cell invasion by clinical isolates of Pseudomonas aeruginosa , 1996, Infection and immunity.

[14]  D. Seal,et al.  Epidemiology of Pseudomonas aeruginosa keratitis in contact lens wearers , 1995, Epidemiology and Infection.

[15]  W. Pitt,et al.  Bacterial Adhesion to Protein-Coated Hydrogels , 1993, Journal of biomaterials applications.

[16]  K. Yamauchi,et al.  Antibacterial activity of lactoferrin and a pepsin-derived lactoferrin peptide fragment , 1993, Infection and immunity.

[17]  M. Tomita,et al.  Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. , 1992, The Journal of applied bacteriology.

[18]  M. Refojo,et al.  Attachment of Pseudomonas to Human-Worn, Disposable Etafilcon A Contact Lenses , 1992, Cornea.

[19]  G. Minno,et al.  Quantitative Analysis of Protein Deposits on Hydrophilic Soft Contact Lenses: I. Comparison to Visual Methods of Analysis. II. Deposit Variation among FDA Lens Material Groups , 1991, Optometry and vision science : official publication of the American Academy of Optometry.

[20]  R. Fullard,et al.  Changes in human tear protein levels with progressively increasing stimulus. , 1991, Investigative ophthalmology & visual science.

[21]  D. Mirejovsky,et al.  Effect of proteins on water and transport properties of various hydrogel contact lens materials. , 1991, Current eye research.

[22]  M. Refojo,et al.  Pseudomonas attachment to low-water and high-water, ionic and nonionic, new and rabbit-worn soft contact lenses. , 1991, Investigative ophthalmology & visual science.

[23]  B. Dunn,et al.  Lactoferrin and transferrin damage of the gram-negative outer membrane is modulated by Ca2+ and Mg2+. , 1990, Journal of general microbiology.

[24]  R. Mandell,et al.  Initial in Vivo Tear Protein Deposition on Individual Hydrogel Contact Lenses , 1990, Optometry and vision science : official publication of the American Academy of Optometry.

[25]  R. Fullard,et al.  Protein levels in nonstimulated and stimulated tears of normal human subjects. , 1990, Investigative ophthalmology & visual science.

[26]  J. Baum,et al.  Bacterial adherence to extended wear soft contact lenses. , 1990, Ophthalmology.

[27]  M. Refojo,et al.  Adherence of Viable and Nonviable Bacteria to Soft Contact Lenses , 1989, Cornea.

[28]  R. Ellison,et al.  Damage of the outer membrane of enteric gram-negative bacteria by lactoferrin and transferrin , 1988, Infection and immunity.

[29]  L A Wilson,et al.  Effects of protein, mucin, and human tears on adherence of Pseudomonas aeruginosa to hydrophilic contact lenses , 1988, Journal of clinical microbiology.

[30]  S. Klotz,et al.  The adherence of Pseudomonas aeruginosa to soft contact lenses. , 1987, Ophthalmology.

[31]  S. Klotz,et al.  Carbohydrate deposits on the surfaces of worn extended-wear soft contact lenses. , 1987, Archives of ophthalmology.

[32]  R. Sack,et al.  Specificity and biological activity of the protein deposited on the hydrogel surface. Relationship of polymer structure to biofilm formation. , 1987, Investigative ophthalmology & visual science.

[33]  Dart Jk,et al.  Bacterial adherence to contact lenses. , 1986 .

[34]  S. Rosenthal.  Local and Systemic Therapy of Pseudomonas Septicemia in Burned Mice , 1967, Annals of surgery.

[35]  R. Millican,et al.  Efficacy of rabbit pseudomonas antiserum in experimental Pseudomonas aeruginosa infection. , 1960, The Journal of infectious diseases.

[36]  B. Holden,et al.  Gram-negative bacteria can induce contact lens related acute red eye (CLARE) responses. , 1996, The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc.

[37]  D. Korb.  Tear film-contact lens interactions. , 1994, Advances in experimental medicine and biology.

[38]  F. Stapleton,et al.  Bacterial adherence and glycocalyx formation onunworn hydrogel lenses , 1993 .

[39]  R. Tripathi,et al.  Analysis of glycoprotein deposits on disposable soft contact lenses. , 1992, Investigative ophthalmology & visual science.

[40]  R. Mandell,et al.  Protein accumulation on disposable extended wear lenses. , 1991, The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc.

[41]  A. Kijlstra.  The role of lactoferrin in the nonspecific immune response on the ocular surface. , 1990, Regional immunology.

[42]  S. Klotz,et al.  Contact lens surface deposits increase the adhesion of Pseudomonas aeruginosa. , 1990, Current eye research.

[43]  E. Alfonso,et al.  Ulcerative keratitis associated with contact lens wear. , 1989, American journal of ophthalmology.

[44]  M. Miller,et al.  Adherence of Pseudomonas aeruginosa to hydrophilic contact lenses and other substrata. , 1987, Journal of clinical microbiology.

[45]  J. Dart,et al.  Bacterial adherence to contact lenses. , 1986, The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc.

[46]  G. Stern,et al.  The pathogenesis of contact lens-associated Pseudomonas aeruginosa corneal ulceration. I. The effect of contact lens coatings on adherence of Pseudomonas aeruginosa to soft contact lenses , 1986 .