The principles of scale space applied to structure and colour in light microscopy

This article shows that scale space theory and spatial colour models can be quantitatively applied to light microscopy images through the use of computer software algorithms. The use of scale space is particularly help- ful in obtaining information about an image in which the conditions were less than optimal, thus reducing the pressure and time during experi- mental setup and data acquisition. In particular, this article considers two dimensional brightfield and fluorescence microscopy. However, the princi- ples described can be applied to other forms of microscopy, including three dimensional images and time series images, thus introducing a number of avenues for assisting in future research.