Comparison of fluorescein and phycoerythrin conjugates for quantifying CD20 expression on normal and leukemic B‐cells

Numerous methods for quantitative fluorescence calibration (QFC) have been developed to quantify receptor expression on lymphocytes as potential disease biomarkers. CD20 expression in B‐cell chronic lymphocytic leukemia (B‐CLL) is one of the best examples of such a biomarker, but results from the use of different QFC methods vary considerably.

[1]  J. Chermann,et al.  Surface CD4 density remains constant on lymphocytes of HIV-infected patients in the progression of disease. , 1991, Research in immunology.

[2]  P. Musto,et al.  Quantitative flow cytometry for the differential diagnosis of leukemic B‐cell chronic lymphoproliferative disorders , 2000, American journal of hematology.

[3]  A. K. Gaigalas,et al.  Quantitating Fluorescence Intensity From Fluorophore: Assignment of MESF Values , 2005, Journal of research of the National Institute of Standards and Technology.

[4]  P. Renzi,et al.  Analysis of T cell subsets in normal adults. Comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry. , 1987, Journal of immunological methods.

[5]  R. Hoffman,et al.  Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation. , 1998, Cytometry.

[6]  M. Grever,et al.  Rituximab using a thrice weekly dosing schedule in B-cell chronic lymphocytic leukemia and small lymphocytic lymphoma demonstrates clinical activity and acceptable toxicity. , 2001, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[7]  L. Ginaldi,et al.  Levels of expression of CD19 and CD20 in chronic B cell leukaemias. , 1998, Journal of clinical pathology.

[8]  G Janossy,et al.  Quantitative analysis of leukocyte membrane antigen expression: normal adult values. , 1996, Cytometry.

[9]  Adolfas K. Gaigalas,et al.  Quantitating Fluorescence Intensity From Fluorophores: Practical Use of MESF Values , 2002, Journal of research of the National Institute of Standards and Technology.

[10]  G. Marti,et al.  CD20 and CD5 Expression in B‐Chronic Lymphocytic Leukemia , 1992, Annals of the New York Academy of Sciences.

[11]  H. Micklem,et al.  Right angle light scatter: a necessary parameter in flow cytofluorimetric analysis of human peripheral blood mononuclear cells. , 1983, Journal of immunological methods.

[12]  T. Denny,et al.  Quantitative determination of surface antibody binding capacities of immune subsets present in peripheral blood of healthy adult donors. , 1996, Cytometry.

[13]  E. Everett,et al.  Reduced expression of CD20 antigen as a characteristic marker for chronic lymphocytic leukemia , 1992, American journal of hematology.

[14]  D Barnett,et al.  Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis. , 1998, Cytometry.