Comparing the Resistance, Resilience, and Stability of Replicate Moving Bed Biofilm and Suspended Growth Combined Nitritation-Anammox Reactors.

Combined partial nitritation-anammox (PN/A) systems are increasingly being employed for sustainable removal of nitrogen from wastewater, but process instabilities present ongoing challenges for practitioners. The goal of this study was to elucidate differences in process stability between PN/A process variations employing two distinct aggregate types: biofilm [in moving bed biofilm reactors (MBBRs)] and suspended growth biomass. Triplicate reactors for each process variation were studied under baseline conditions and in response to a series of transient perturbations. MBBRs displayed elevated NH4+ removal rates relative to those of suspended growth counterparts over six months of unperturbed baseline operation but also exhibited significantly greater variability in performance. Transient perturbations led to strikingly divergent yet reproducible behavior in biofilm versus suspended growth systems. A temperature perturbation prompted a sharp reduction in NH4+ removal rates with no accumulation of NO2- and rapid recovery in MBBRs, compared to a similar reduction in NH4+ removal rates but a high level of accumulation of NO2- in suspended growth reactors. Pulse additions of a nitrification inhibitor (allylthiourea) prompted only moderate declines in performance in suspended growth reactors compared to sharp decreases in NH4+ removal rates in MBBRs. Quantitative fluorescence in situ hybridization demonstrated a significant enrichment of anammox in MBBRs compared to suspended growth reactors, and conversely a proportionally higher AOB abundance in suspended growth reactors. Overall, MBBRs displayed significantly increased susceptibility to transient perturbations employed in this study compared to that of suspended growth counterparts (stability parameter), including significantly longer recovery times (resilience). No significant difference in the maximal impact of perturbations (resistance) was apparent. Taken together, our results suggest that aggregate architecture (biofilm vs suspended growth) in PN/A processes exerts an unexpectedly strong influence on process stability.

[1]  C. Vannini,et al.  Kinetic parameters and inhibition response of ammonia‐ and nitrite‐oxidizing bacteria in membrane bioreactors and conventional activated sludge processes , 2010, Environmental technology.

[2]  H. Insam,et al.  Identification of anammox bacteria in a full-scale deammonification plant making use of anaerobic ammonia oxidation. , 2007, Systematic and applied microbiology.

[3]  Susanne Lackner,et al.  Evaluating operation strategies and process stability of a single stage nitritation-anammox SBR by use of the oxidation-reduction potential (ORP). , 2012, Bioresource technology.

[4]  Anneli Andersson Chan,et al.  Experience from start-ups of the first ANITA Mox plants. , 2013, Water science and technology : a journal of the International Association on Water Pollution Research.

[5]  H. Siegrist,et al.  Full-scale nitrogen removal from digester liquid with partial nitritation and anammox in one SBR. , 2009, Environmental science & technology.

[6]  P. Nielsen,et al.  FISH Handbook for Biological Wastewater Treatment , 2009 .

[7]  K. Rosenwinkel,et al.  Deammonification in the Moving‐Bed Process for the Treatment of Wastewater with High Ammonia Content , 2005 .

[8]  S. Lackner,et al.  Comparing different reactor configurations for Partial Nitritation/Anammox at low temperatures. , 2015, Water research.

[9]  M. V. van Loosdrecht,et al.  The effect of nitrite inhibition on the anammox process. , 2012, Water research.

[10]  D. Gao,et al.  Versatility and application of anaerobic ammonium-oxidizing bacteria , 2011, Applied Microbiology and Biotechnology.

[11]  B Wett,et al.  Development and implementation of a robust deammonification process. , 2007, Water science and technology : a journal of the International Association on Water Pollution Research.

[12]  M Christensson,et al.  Integrated fixed-film activated sludge ANITA™Mox process--a new perspective for advanced nitrogen removal. , 2014, Water science and technology : a journal of the International Association on Water Pollution Research.

[13]  G. Wells,et al.  Microbial activity balance in size fractionated suspended growth biomass from full-scale sidestream combined nitritation-anammox reactors. , 2016, Bioresource technology.

[14]  Christian Wissel,et al.  Babel, or the ecological stability discussions: an inventory and analysis of terminology and a guide for avoiding confusion , 1997, Oecologia.

[15]  Yongzhen Peng,et al.  Biological nitrogen removal from sewage via anammox: Recent advances. , 2016, Bioresource technology.

[16]  M. V. van Loosdrecht,et al.  Nitrate reduction by organotrophic Anammox bacteria in a nitritation/anammox granular sludge and a moving bed biofilm reactor. , 2012, Bioresource technology.

[17]  Mark C M van Loosdrecht,et al.  Segregation of biomass in cyclic anaerobic/aerobic granular sludge allows the enrichment of anaerobic ammonium oxidizing bacteria at low temperatures. , 2011, Environmental science & technology.

[18]  Zhiguo Yuan,et al.  Determination of Growth Rate and Yield of Nitrifying Bacteria by Measuring Carbon Dioxide Uptake Rate , 2007, Water environment research : a research publication of the Water Environment Federation.

[19]  Christian Wissel,et al.  On the application of stability concepts in ecology , 1992 .

[20]  Joshua P Boltz,et al.  Moving Bed Biofilm Reactor Technology: Process Applications, Design, and Performance , 2011, Water environment research : a research publication of the Water Environment Federation.

[21]  Susanne Lackner,et al.  Full-scale partial nitritation/anammox experiences--an application survey. , 2014, Water research.

[22]  H. Siegrist,et al.  Combined nitritation-anammox: advances in understanding process stability. , 2011, Environmental science & technology.

[23]  Lesley A. Robertson,et al.  Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor , 1996 .

[24]  R. Méndez,et al.  Evaluation of activity and inhibition effects on Anammox process by batch tests based on the nitrogen-gas production , 2007 .

[25]  B Wett,et al.  Syntrophy of aerobic and anaerobic ammonia oxidisers. , 2010, Water science and technology : a journal of the International Association on Water Pollution Research.

[26]  R. Knowles,et al.  Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers , 1989, Microbiological reviews.

[27]  N. Jardin,et al.  Full-scale experience with the deammonification process to treat high strength sludge water -- a case study. , 2012, Water science and technology : a journal of the International Association on Water Pollution Research.

[28]  Willy Verstraete,et al.  Aggregate Size and Architecture Determine Microbial Activity Balance for One-Stage Partial Nitritation and Anammox , 2009, Applied and Environmental Microbiology.

[29]  M. V. van Loosdrecht,et al.  Effect of temperature change on anammox activity , 2015, Biotechnology and Bioengineering.

[30]  Jean-Louis Fanlo,et al.  Resistance and resilience of removal efficiency and bacterial community structure of gas biofilters exposed to repeated shock loads. , 2012, Bioresource technology.

[31]  P. Zheng,et al.  Quantitative comparison of stability of ANAMMOX process in different reactor configurations. , 2008, Bioresource technology.

[32]  H. Caswell,et al.  ALTERNATIVES TO RESILIENCE FOR MEASURING THE RESPONSES OF ECOLOGICAL SYSTEMS TO PERTURBATIONS , 1997 .

[33]  M C M van Loosdrecht,et al.  Simultaneous partial nitritation and anammox at low temperature with granular sludge. , 2014, Water research.

[34]  Didier L. Baho,et al.  Fundamentals of Microbial Community Resistance and Resilience , 2012, Front. Microbio..

[35]  M. V. van Loosdrecht,et al.  Startup of reactors for anoxic ammonium oxidation: experiences from the first full-scale anammox reactor in Rotterdam. , 2007, Water research.

[36]  C. Criddle,et al.  Correlation of patterns of denitrification instability in replicated bioreactor communities with shifts in the relative abundance and the denitrification patterns of specific populations , 2007, The ISME Journal.

[37]  M. Strous,et al.  Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor. , 2005, FEMS microbiology ecology.

[38]  M. V. van Loosdrecht,et al.  Effect of heterotrophic growth on autotrophic nitrogen removal in a granular sludge reactor , 2014, Environmental technology.

[39]  P. Zheng,et al.  The inhibition of the Anammox process: A review , 2012 .

[40]  S. Lackner,et al.  Comparing the performance and operation stability of an SBR and MBBR for single-stage nitritation-anammox treating wastewater with high organic load , 2013, Environmental technology.

[41]  J. Nielsen,et al.  Activity and growth of anammox biomass on aerobically pre-treated municipal wastewater , 2015, Water research.

[42]  Craig S. Criddle,et al.  Parallel Processing of Substrate Correlates with Greater Functional Stability in Methanogenic Bioreactor Communities Perturbed by Glucose , 2000, Applied and Environmental Microbiology.

[43]  W. Verstraete,et al.  Microbial resource management of one‐stage partial nitritation/anammox , 2012, Microbial biotechnology.

[44]  J. A. Field,et al.  Nitrite (not free nitrous acid) is the main inhibitor of the anammox process at common pH conditions , 2013, Biotechnology Letters.

[45]  E Morgenroth,et al.  Impact of coexistence of flocs and biofilm on performance of combined nitritation-anammox granular sludge reactors. , 2015, Water research.

[46]  Mark C M van Loosdrecht,et al.  Sensitivity analysis of a biofilm model describing a one-stage completely autotrophic nitrogen removal (CANON) process. , 2002, Biotechnology and bioengineering.

[47]  Han Gao,et al.  Towards energy neutral wastewater treatment: methodology and state of the art. , 2014, Environmental science. Processes & impacts.

[48]  J. Field,et al.  Inhibition of anaerobic ammonium oxidizing (anammox) enrichment cultures by substrates, metabolites and common wastewater constituents. , 2013, Chemosphere.