Surtos de doença vesicular causados pelo Vaccinia virus em bovinos de leite no Estado de Goiás, Brasil (2010-2012)

ABSTRACT.- Sant’Ana F.J.F., Leal A.A., Rabelo R.E., Vulcani V.A.S., Ferreira Junior J.A., Carg-nelutti J.F. & Flores E.F. 2013. Outbreaks of vesicular disease caused by Vaccinia virus in dairy cattle from Goias State, Brazil (2010-2012) . Pesquisa Veterinaria Brasileira 33(7):860-866. Laboratorio de Patologia Veterinaria, Universidade de Brasilia, Brasilia, DF 70910-900, Brazil. E-mail: santanafjf@yahoo.comCases of vesicular and exanthematic disease by Vaccinia virus (VACV) have been reported in dairy herds of several Brazilian regions, occasionally also affecting humans. The present article describes eight outbreaks of vesicular disease caused by VACV in dairy herds of six counties of Goias state, Midwestern Brazil (2010-2012), involving a total of 122 cows, 12 calves and 11 people. Dairy cows (3 to 9 years old) were affected in all cases and calves (2 to 9 months old) were affected in five outbreaks, presenting oral lesions. The morbidity ranged between 8 and 100% in cows, and 1.5 to 31% in calves. In the cows, the clinical signs started with vesicles (2-7mm), painful and coalescent papules (3-8 mm), which resulted in ulcers (5-25mm) and scabs in teats, and, occasionally, in the muzzle. The clinical course lasted from 16 to 26 days. The histopathology of bovine skin samples revealed superficial perivascular in-flammatory infiltrate of lymphocytes, plasma cells, neutrophils, macrophages and multifocal areas of acanthosis, spongiosis, hipergranulosis and parakeratotic or orthokeratotic hyperke-ratosis with adjacent focally extensive ulcers. Eosinophilic inclusion bodies were noted in the cytoplasm of the keratinocytes. PCR to

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