Force-controlled spatial manipulation of viable mammalian cells and micro-organisms by means of FluidFM technology

The FluidFM technology uses microchanneled atomic force microscope cantilevers that are fixed to a drilled atomic force microscope cantilevers probeholder. A continuous fluidic circuit is thereby achieved extending from an external liquid reservoir, through the probeholder and the hollow cantilever to the tip aperture. In this way, both overpressure and an underpressure can be applied to the liquid reservoir and hence to the built-in fluidic circuit. We describe in this letter how standard atomic force microscopy in combination with regulated pressure differences inside the microchanneled cantilevers can be used to displace living organisms with micrometric precision in a nondestructive way. The protocol is applicable to both eukaryotic and prokaryotic cells (e.g., mammalian cells, yeasts, and bacteria) in physiological buffer. By means of this procedure, cells can also be transferred from one glass slide to another one or onto an agar medium.

[1]  Ulrich S. Schwarz,et al.  Probing cellular microenvironments and tissue remodeling by atomic force microscopy , 2008, Pflügers Archiv - European Journal of Physiology.

[2]  Julia Gorelik,et al.  Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette. , 2008, Biophysical journal.

[3]  A. Ashkin,et al.  Internal cell manipulation using infrared laser traps. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[4]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[5]  Gijsbertus J.M. Krijnen,et al.  Micromachined fountain pen for atomic force microscope-based nanopatterning , 2004 .

[6]  Jay X. Tang,et al.  Adhesion of single bacterial cells in the micronewton range. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Hermann E. Gaub,et al.  Discrete interactions in cell adhesion measured by single-molecule force spectroscopy , 2000, Nature Cell Biology.

[8]  B. Tromberg,et al.  Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption. , 1996, Optics letters.

[9]  Horacio D Espinosa,et al.  A nanofountain probe with Sub-100 nm molecular writing resolution. , 2005, Small.

[10]  K. Svoboda,et al.  Biological applications of optical forces. , 1994, Annual review of biophysics and biomolecular structure.

[11]  Clemens M. Franz,et al.  Atomic Force Microscopy: A Versatile Tool for Studying Cell Morphology, Adhesion and Mechanics , 2008 .

[12]  M. Sheetz,et al.  Local force and geometry sensing regulate cell functions , 2006, Nature Reviews Molecular Cell Biology.

[13]  V. Moy,et al.  Atomic force microscopy measurement of leukocyte-endothelial interaction. , 2004, American journal of physiology. Heart and circulatory physiology.

[14]  K Bergman,et al.  Characterization of photodamage to Escherichia coli in optical traps. , 1999, Biophysical journal.

[15]  C. Quate,et al.  Forces in atomic force microscopy in air and water , 1989 .

[16]  Gijsbertus J.M. Krijnen,et al.  Fabrication of micromachined fountain pen with in situ characterization possibility of nanoscale surface modification , 2005 .

[17]  R. Hochmuth,et al.  Micropipette aspiration of living cells. , 2000, Journal of biomechanics.

[18]  L. Oddershede,et al.  Optical Tweezers Cause Physiological Damage to Escherichia coli and Listeria Bacteria , 2008, Applied and Environmental Microbiology.

[19]  U. Schwarz,et al.  Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors. , 2010, Biophysical journal.

[20]  Marcus Textor,et al.  Poly(l-lysine)-g-poly(ethylene glycol) Layers on Metal Oxide Surfaces: Surface-Analytical Characterization and Resistance to Serum and Fibrinogen Adsorption , 2001 .

[21]  H. Gaub,et al.  Print your atomic force microscope. , 2007, The Review of scientific instruments.

[22]  Dino Di Carlo,et al.  Dynamic single-cell analysis for quantitative biology. , 2006, Analytical chemistry.

[23]  Michael W. Berns,et al.  Radiation trapping forces on microspheres with optical tweezers , 1993 .

[24]  Mattias Goksör,et al.  Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. , 2005, Lab on a chip.

[25]  Takayuki Shibata,et al.  Micromachining of a newly designed AFM probe integrated with hollow microneedle for cellular function analysis , 2010 .

[26]  A. Ashkin,et al.  Optical trapping and manipulation of single cells using infrared laser beams , 1987, Nature.

[27]  Nicolas Blondiaux,et al.  Use of force spectroscopy to investigate the adhesion of living adherent cells. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[28]  T K Chowdhury,et al.  Fabrication of extremely fine glass micropipette electrodes. , 1969, Journal of scientific instruments.

[29]  Tomaso Zambelli,et al.  FluidFM: combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond. , 2009, Nano letters.

[30]  K. Greulich,et al.  Manipulation of cells, organelles, and genomes by laser microbeam and optical trap. , 1992, International review of cytology.