Effects of cryopreservation on bone marrow derived mesenchymal cells of a nonhuman primate.

Bone marrow stromal cells (MSCs) have multi-lineage differentiation capabilities and are focused on as a cell source for various cell therapies. To facilitate the availability of MSCs, cryopreservation technique is one of the critical factors for the cell therapies. In this study, effects of cryopreservation on capabilities of MSCs derived from a nonhuman primate were tested, aimed at a clinical application for tissue-engineered bone reconstruction. Effects of cryopreservation on the MSCs' adhesion rate, proliferation, and osteogenic differentiation in vitro were compared with non-cryopreserved MSCs. Bone formation capabilities were also tested using an extraskeletal bone induction model. The bone formation inducted by the combination of cryopreserved MSCs and an artificial bone scaffold was confirmed in all cases. The amount of bone formation at each case was irregular, but the results were suggested the possibility of cryopreserved MSCs on clinical use.

[1]  H. Ohgushi,et al.  Tissue-engineered approach for the treatment of steroid-induced osteonecrosis of the femoral head: transplantation of autologous mesenchymal stem cells cultured with beta-tricalcium phosphate ceramics and free vascularized fibula. , 2006, Artificial organs.

[2]  Zhao-qing Guo,et al.  [Lumbar interbody fusion using autologous bone marrow mesenchymal stem cell-calcium phosphate ceramic composite in rhesus monkey]. , 2006, Zhonghua wai ke za zhi [Chinese journal of surgery].

[3]  H. Ohgushi,et al.  Tissue engineering approach to the treatment of bone tumors: three cases of cultured bone grafts derived from patients' mesenchymal stem cells. , 2006, Artificial organs.

[4]  Techung Lee,et al.  Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture , 2005, Journal of cellular physiology.

[5]  Eleftherios Tsiridis,et al.  Bone substitutes: an update. , 2005, Injury.

[6]  H. Ohgushi,et al.  Tissue engineered ceramic artificial joint--ex vivo osteogenic differentiation of patient mesenchymal cells on total ankle joints for treatment of osteoarthritis. , 2005, Biomaterials.

[7]  Hajime Ohgushi,et al.  Viability and osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells. , 2005, Tissue engineering.

[8]  T. Uemura,et al.  Correlation of in vivo bone formation capability and in vitro differentiation of human bone marrow stromal cells. , 2005, Journal of medical and dental sciences.

[9]  K. Shinomiya,et al.  Beta-tricalcium phosphate (beta-TCP) graft combined with bone marrow stromal cells (MSCs) for posterolateral spine fusion. , 2005, Journal of medical and dental sciences.

[10]  H. Nakajima,et al.  Osteogenesis with cryopreserved marrow mesenchymal cells. , 2005, Tissue engineering.

[11]  H. Ohgushi,et al.  Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells after thawing in culture , 2004 .

[12]  A. Osyczka,et al.  Different Effects of BMP-2 on Marrow Stromal Cells from Human and Rat Bone , 2004, Cells Tissues Organs.

[13]  S. Both,et al.  Bone tissue-engineered implants using human bone marrow stromal cells: effect of culture conditions and donor age. , 2002, Tissue engineering.

[14]  S. Gerson,et al.  Allogeneic mesenchymal stem cell infusion for treatment of metachromatic leukodystrophy (MLD) and Hurler syndrome (MPS-IH) , 2002, Bone Marrow Transplantation.

[15]  R. Legeros,et al.  Properties of osteoconductive biomaterials: calcium phosphates. , 2002, Clinical orthopaedics and related research.

[16]  R. Bucholz.  Nonallograft osteoconductive bone graft substitutes. , 2002, Clinical orthopaedics and related research.

[17]  W. Koo,et al.  Clinical responses to bone marrow transplantation in children with severe osteogenesis imperfecta. , 2001, Blood.

[18]  R. Class,et al.  Propagation and senescence of human marrow stromal cells in culture: a simple colony‐forming assay identifies samples with the greatest potential to propagate and differentiate , 1999, British journal of haematology.

[19]  M. Pittenger,et al.  Multilineage potential of adult human mesenchymal stem cells. , 1999, Science.

[20]  Darwin J. Prockop,et al.  Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta , 1999, Nature Medicine.

[21]  S Tamai,et al.  The Effect of Aging on Bone Formation in Porous Hydroxyapatite: Biochemical and Histological Analysis , 1997, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[22]  D. Prockop.  Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues , 1997, Science.

[23]  S. Bruder,et al.  Growth kinetics, self‐renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation , 1997, Journal of cellular biochemistry.

[24]  Marc A. Asher,et al.  Iliac Crest Bone Graft Harvest Donor Site Morbidity: A Statistical Evaluation , 1995, Spine.

[25]  Banwart Jc,et al.  Iliac crest bone graft harvest donor site morbidity. A statistical evaluation. , 1995 .

[26]  A. Levine,et al.  Chronic Donor Site Pain Complicating Bone Graft Harvesting From the Posterior Iliac Crest for Spinal Fusion , 1992, Spine.

[27]  B. Schick.  Synthesis of proteins from [35S]methionine by guinea pig megakaryocytes in vivo and time course of appearance of newly synthesized proteins in platelets. , 1990, Blood.

[28]  R E Booth,et al.  Harvesting Autogenous Iliac Bone Grafts: A Review of Complications and Techniques , 1989, Spine.

[29]  K. de Groot,et al.  Immediate dental root implants from synthetic dense calcium hydroxylapatite. , 1979, The Journal of prosthetic dentistry.

[30]  Zhao-qing Guo,et al.  Evaluation of autologous bone marrow mesenchymal stem cell-calcium phosphate ceramic composite for lumbar fusion in rhesus monkey interbody fusion model. , 2005, Tissue engineering.

[31]  S. Gerson,et al.  Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy. , 2000, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.