论文信息 - YOCTOMOLE NON-LABELED PROTEIN DETECTION IN EXTENDED-NANO CHANNEL BY UV EXCITATION DIFFERENTIAL INTERFERENCE CONTRAST THERMAL LENS MICROSCOPY

YOCTOMOLE NON-LABELED PROTEIN DETECTION IN EXTENDED-NANO CHANNEL BY UV EXCITATION DIFFERENTIAL INTERFERENCE CONTRAST THERMAL LENS MICROSCOPY

We report non-labeled protein detection at 100 yoctomole (10 mole) level with an optical path length of 900 nm by improving UV differential interference contrast thermal lens microscopy (UV-DICTLM). In the present study, we made UV-DIC-TLM optimized the optical configuration to obtain maximum signal, and realized 15-fold higher sensitivity than previous report [1].

K. Mawatari | T. Kitamori | H. Shimizu | N. Miyawaki

[1] Takehiko Kitamori,et al. Highly efficient and ultra-small volume separation by pressure-driven liquid chromatography in extended nanochannels. , 2012, Small.

[2] K. Mawatari,et al. Sensitive determination of concentration of nonfluorescent species in an extended-nano channel by differential interference contrast thermal lens microscope. , 2010, Analytical chemistry.

[3] K. Mawatari,et al. DETECTION OF ZEPTOMOLE NONLABELED PROTEIN IN EXTENDED-NANO CHANNEL USING UV EXCITATION DIFFERENTIAL INTERFERENCE CONTRAST THERMAL LENS MICROSCOPE ( DIC-TLM ) , 2014 .