CDw50 and ICAM‐3: Two names for the same molecule

CDw50 differentiation antigen is a molecule broadly expressed on hematopoetic cells but not on other cells. Previous experiments showed that CDw50 monoclonal antibodies (mAb) inhibited primary mixed lymphocyte culture (MLC). To understand the function of CDw50 better, we purified it and obtained peptide sequence. At the same time, intercellular adhesion molecule (ICAM)‐3, the third Iigand of lymphocyte function‐associated molecule 1, was described by mAb and subsequent cDNA cloning. Immunochemical, functional, and protein sequencing studies show that ICAM‐3 and CDw50 are the same glycoprotein, a 120‐kDa surface molecule with presumably an important role in the immune responses.

[1]  T. Springer,et al.  Cloning and expression of intercellular adhesion molecule 3 reveals strong homology to other immunoglobulin family counter-receptors for lymphocyte function-associated antigen 1 , 1993, The Journal of experimental medicine.

[2]  R. Jasman,et al.  Cloning and characterization of a new intercellular adhesion molecule ICAM-R , 1992, Nature.

[3]  D. Mason,et al.  Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytes , 1992, Nature.

[4]  T. Springer,et al.  Intercellular adhesion molecule 3, a third adhesion counter-receptor for lymphocyte function-associated molecule 1 on resting lymphocytes , 1992, The Journal of experimental medicine.

[5]  J. Alberola-Ila,et al.  Effect of protein kinase C activators on the phosphorylation and the surface expression of the CDw50 leukocyte antigen. , 1992, European journal of biochemistry.

[6]  T. Springer,et al.  Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1 , 1991, The Journal of experimental medicine.

[7]  J. Cambier,et al.  IgM antigen receptor complex contains phosphoprotein products of B29 and mb-1 genes. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[8]  Michael Loran Dustin,et al.  Role of lymphocyte adhesion receptors in transient interactions and cell locomotion. , 1991, Annual review of immunology.

[9]  M. Diamond,et al.  ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18) , 1990, The Journal of cell biology.

[10]  R. Vilella,et al.  Involvement of the CDw50 molecule in allorecognition. , 1990, Tissue antigens.

[11]  Timothy A. Springer,et al.  Adhesion receptors of the immune system , 1990, Nature.

[12]  H. Okayama,et al.  High-efficiency transformation of mammalian cells by plasmid DNA. , 1987, Molecular and cellular biology.

[13]  I. Bernstein,et al.  Leukocyte Typing II , 1986, Springer New York.

[14]  J. Strominger,et al.  Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[15]  T. Springer,et al.  Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. , 1982, The Journal of biological chemistry.

[16]  Fox Cf,et al.  Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. , 1978 .

[17]  C. Barnstable,et al.  Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis , 1978, Cell.

[18]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.