Polyhydroxyalkanoate (PHA) Synthases: The Key Enzymes of PHA Synthesis
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[1] A. Steinbüchel,et al. Characterization of the Polyhydroxyalkanoate Synthase Gene Locus of Rhodobacter Sphaeroides , 1993, Biotechnology Letters.
[2] R. Doolittle,et al. Progressive sequence alignment as a prerequisitetto correct phylogenetic trees , 2007, Journal of Molecular Evolution.
[3] D. Solaiman. PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli , 2000, Biotechnology Letters.
[4] A. Steinbüchel,et al. Anaylsis of polyhydroxyalkanoic acid-biosynthesis genes of anoxygenic phototrophic bacteria reveals synthesis of a polyester exhibiting an unusal composition , 1993, Applied Microbiology and Biotechnology.
[5] Yoshiharu Doi,et al. Substrate specificities in hydrolysis of polyhydroxyalkanoates by microbial esterases , 1993, Biotechnology Letters.
[6] A. Steinbüchel,et al. Application of enzymatically synthesized short-chain-length hydroxy fatty acid coenzyme A thioesters for assay of polyhydroxyalkanoic acid synthases , 2004, Applied Microbiology and Biotechnology.
[7] S. Wiese,et al. A general method for identification of polyhydroxyalkanoic acid synthase genes from pseudomonads belonging to the rRNA homology group I , 2004, Applied Microbiology and Biotechnology.
[8] A. Steinbüchel,et al. Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthetic genes of Thiocystis violacea , 2004, Applied Microbiology and Biotechnology.
[9] H. Schlegel,et al. The isolation of mutants not accumulating poly-β-hydroxybutyric acid , 2004, Archiv für Mikrobiologie.
[10] A. Steinbüchel,et al. Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model. , 2002, Biochimica et biophysica acta.
[11] Q. Qi,et al. Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: molecular characterization of the polyhydroxybutyrate synthase. , 2001, Microbiology.
[12] A. Steinbüchel. PHB and Other Polhydroxyalkanoic Acids , 2001 .
[13] H. Nakamura,et al. Analysis of mutational effects of a polyhydroxybutyrate (PHB) polymerase on bacterial PHB accumulation using an in vivo assay system. , 2001, FEMS microbiology letters.
[14] A. Sinskey,et al. Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: class I and III synthases share a similar catalytic mechanism. , 2001, Biochemistry.
[15] A. Steinbüchel,et al. Biochemical and molecular basis of microbial synthesis of polyhydroxyalkanoates in microorganisms. , 2001, Advances in biochemical engineering/biotechnology.
[16] A. Steinbüchel,et al. Identification of a new class of biopolymer: bacterial synthesis of a sulfur-containing polymer with thioester linkages. , 2001, Microbiology.
[17] A. Steinbüchel,et al. Studies on polyhydroxyalkanoate (PHA) accumulation in a PHA synthase I-negative mutant of Burkholderia cepacia generated by homogenotization. , 2000, FEMS microbiology letters.
[18] A. Steinbüchel,et al. Homologous functional expression of cryptic phaG from Pseudomonas oleovorans establishes the transacylase-mediated polyhydroxyalkanoate biosynthetic pathway , 2000, Applied Microbiology and Biotechnology.
[19] A. Steinbüchel,et al. Analysis of the Thiocapsa pfennigii polyhydroxyalkanoate synthase: subcloning, molecular characterization and generation of hybrid synthases with the corresponding Chromatium vinosum enzyme , 2000, Applied Microbiology and Biotechnology.
[20] S. Salzberg,et al. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae , 2000, Nature.
[21] A. Steinbüchel,et al. In vitro synthesis of poly(3-hydroxydecanoate): purification and enzymatic characterization of type II polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa , 2000, Applied Microbiology and Biotechnology.
[22] A. Steinbüchel,et al. Exploitation of butyrate kinase and phosphotransbutyrylase from Clostridium acetobutylicum for the in vitro biosynthesis of poly(hydroxyalkanoic acid) , 2000, Applied Microbiology and Biotechnology.
[23] A. Steinbüchel,et al. PhaG-Mediated Synthesis of Poly(3-Hydroxyalkanoates) Consisting of Medium-Chain-Length Constituents from Nonrelated Carbon Sources in Recombinant Pseudomonas fragi , 2000, Applied and Environmental Microbiology.
[24] A. Steinbüchel,et al. Polyhydroxyalkanoate accumulation in Burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids , 2000, Applied Microbiology and Biotechnology.
[25] T. Clemente,et al. Sequence of PHA synthase gene from two strains of Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across two heterologous expression systems , 2000, Applied Microbiology and Biotechnology.
[26] A. Sinskey,et al. Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase. , 2000, Biochemistry.
[27] T. Fukui,et al. Molecular cloning of two (R)-specific enoyl-CoA hydratase genes from Pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis. , 2000, FEMS microbiology letters.
[28] A. Steinbüchel,et al. The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources. , 2000, FEMS microbiology letters.
[29] A. Steinbüchel,et al. A Novel Genetically Engineered Pathway for Synthesis of Poly(Hydroxyalkanoic Acids) in Escherichia coli , 2000, Applied and Environmental Microbiology.
[30] A. Steinbüchel,et al. Analysis of in vivo substrate specificity of the PHA synthase from Ralstonia eutropha: formation of novel copolyesters in recombinant Escherichia coli. , 2000, FEMS microbiology letters.
[31] J. García,et al. Novel Biodegradable Aromatic Plastics from a Bacterial Source , 1999, The Journal of Biological Chemistry.
[32] A. Miller,et al. Cloning, Molecular Analysis, and Expression of the Polyhydroxyalkanoic Acid Synthase (phaC) Gene fromChromobacterium violaceum , 1999, Applied and Environmental Microbiology.
[33] R. Weusthuis,et al. Development of environmentally friendly coatings and paints using medium-chain-length poly(3-hydroxyalkanoates) as the polymer binder. , 1999, International journal of biological macromolecules.
[34] A. Steinbüchel,et al. Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis. , 1999, International journal of biological macromolecules.
[35] David P. Martin,et al. PHA applications: addressing the price performance issue: I. Tissue engineering. , 1999, International journal of biological macromolecules.
[36] T. Yamane,et al. Analyses of a Polyhydroxyalkanoic Acid Granule-Associated 16-Kilodalton Protein and Its Putative Regulator in the pha Locus of Paracoccus denitrificans , 1999, Journal of bacteriology.
[37] A. Steinbüchel,et al. Analysis of 4-Phosphopantetheinylation of Polyhydroxybutyrate Synthase from Ralstonia eutropha: Generation of β-Alanine Auxotrophic Tn5 Mutants and Cloning of thepanD Gene Region , 1999, Journal of bacteriology.
[38] M. Cannon,et al. Polyhydroxyalkanoate Inclusion Body-Associated Proteins and Coding Region in Bacillus megaterium , 1999, Journal of bacteriology.
[39] A. Steinbüchel,et al. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds , 1999, Archives of Microbiology.
[40] William Arbuthnot Sir Lane,et al. PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis. , 1999, Biochemistry.
[41] T. Fukui,et al. Co-expression of polyhydroxyalkanoate synthase and (R)-enoyl-CoA hydratase genes of Aeromonas caviae establishes copolyester biosynthesis pathway in Escherichia coli. , 1999, FEMS microbiology letters.
[42] N. Rawlings,et al. Identification of the active site of legumain links it to caspases, clostripain and gingipains in a new clan of cysteine endopeptidases , 1998, FEBS letters.
[43] T. Fukui,et al. Cloning and Molecular Analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) Biosynthesis Genes in Pseudomonas sp. Strain 61-3 , 1998, Journal of bacteriology.
[44] S. Lee,et al. Cloning of the Alcaligenes latus Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in Escherichia coli , 1998, Applied and Environmental Microbiology.
[45] T. Sicheritz-Pontén,et al. The genome sequence of Rickettsia prowazekii and the origin of mitochondria , 1998, Nature.
[46] A. Steinbüchel,et al. In vitro synthesis of poly(3-hydroxybutyric acid) by using an enzymatic coenzyme A recycling system. , 1998, FEMS microbiology letters.
[47] Richard Hughey,et al. Hidden Markov models for detecting remote protein homologies , 1998, Bioinform..
[48] R. Lenz,et al. Investigation of the function of proteins associated to polyhydroxyalkanoate inclusions in Pseudomonas putida BMO1. , 1998, Journal of biotechnology.
[49] H. Valentin,et al. Formation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by PHA synthase from Ralstonia eutropha. , 1998, Journal of biotechnology.
[50] A. Steinbüchel,et al. Bacterial and other biological systems for polyester production. , 1998, Trends in biotechnology.
[51] A. Steinbüchel,et al. Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): inhibition of fatty acid beta-oxidation by acrylic acid. , 1998, FEMS microbiology letters.
[52] S. Encarnación,et al. Poly-β-Hydroxybutyrate Turnover inAzorhizobium caulinodans Is Required for Growth and AffectsnifA Expression , 1998, Journal of bacteriology.
[53] A. Steinbüchel,et al. A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis. The PHAG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier protein-coenzyme a transferase. , 1998, The Journal of biological chemistry.
[54] T. Fukui,et al. Genetic Analysis of Comamonas acidovoransPolyhydroxyalkanoate Synthase and Factors Affecting the Incorporation of 4-Hydroxybutyrate Monomer , 1998, Applied and Environmental Microbiology.
[55] A. Steinbüchel,et al. Synechocystis sp. PCC6803 possesses a two-component polyhydroxyalkanoic acid synthase similar to that of anoxygenic purple sulfur bacteria , 1998, Archives of Microbiology.
[56] K. Houmiel,et al. Multiple β-Ketothiolases Mediate Poly(β-Hydroxyalkanoate) Copolymer Synthesis in Ralstonia eutropha , 1998 .
[57] A. Steinbüchel,et al. In vitro biosynthesis of poly(3-hydroxybutyric acid) by using purified poly(hydroxyalkanoic acid) synthase of Chromatium vinosum , 1998, Applied Microbiology and Biotechnology.
[58] T. Fukui,et al. Expression and Characterization of (R)-Specific Enoyl Coenzyme A Hydratase Involved in Polyhydroxyalkanoate Biosynthesis by Aeromonas caviae , 1998, Journal of bacteriology.
[59] A. Steinbüchel,et al. Biosynthesis of polyesters in bacteria and recombinant organisms , 1998 .
[60] A. Steinbüchel,et al. Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate) synthesis. , 2006, FEMS microbiology letters.
[61] A. Steinbüchel,et al. Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2. , 1997, FEMS microbiology letters.
[62] B. Witholt,et al. In vitro activities of granule-bound poly[(R)-3-hydroxyalkanoate]polymerase C1 of Pseudomonas oleovorans--development of an activity test for medium-chain-length-poly(3-hydroxyalkanoate) polymerases. , 1997, European journal of biochemistry.
[63] M. Madigan,et al. Polyhydroxyalkanoate production in Rhodobacter capsulatus: genes, mutants, expression, and physiology , 1997, Applied and environmental microbiology.
[64] T. Fukui,et al. Cloning and Analysis of the Poly ( 3-Hydroxybutyrate-co3-Hydroxyhexanoate ) Biosynthesis Genes of Aeromonas caviae , 1996 .
[65] H. Abe,et al. Molecular mass of poly[(R )-3-hydroxybutyric acid] produced in a recombinant Escherichia coli , 1997, Applied Microbiology and Biotechnology.
[66] N. Guex,et al. SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modeling , 1997, Electrophoresis.
[67] J. Schrag,et al. Lipases and alpha/beta hydrolase fold. , 1997, Methods in enzymology.
[68] A. Sinskey,et al. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo , 1997, Nature Biotechnology.
[69] A. Steinbüchel,et al. Investigation of poly ( β-L-malic acid) production by strains of Aureobasidium pullulans , 1996, Applied Microbiology and Biotechnology.
[70] K. Biemann,et al. Polyhydroxybutyrate Synthase: Evidence for Covalent Catalysis , 1996 .
[71] D. Fischer,et al. Protein fold recognition using sequence‐derived predictions , 1996, Protein science : a publication of the Protein Society.
[72] S. Encarnación,et al. Genetic and physiological characterization of a Rhizobium etli mutant strain unable to synthesize poly-beta-hydroxybutyrate , 1996, Journal of bacteriology.
[73] T. Yamane,et al. Molecular analysis of the poly(3-hydroxyalkanoate) synthase gene from a methylotrophic bacterium, Paracoccus denitrificans , 1996, Journal of bacteriology.
[74] D. Jendrossek,et al. Biodegradation of polyhydroxyalkanoic acids , 1996, Applied Microbiology and Biotechnology.
[75] O. Peoples,et al. Biodegradable plastics from plants , 1996 .
[76] A. Steinbüchel,et al. Electron microscopic observations on the macromolecular organization of the boundary layer of bacterial PHA inclusion bodies , 1996 .
[77] C. Föllner,et al. Considerations on the structure and biochemistry of bacterial polyhydroxyalkanoic acid inclusions. , 1995, Canadian journal of microbiology.
[78] A. Squartini,et al. Poly-β-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti 41 , 1995 .
[79] D. Jendrossek,et al. Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates) , 1995, Applied and environmental microbiology.
[80] T. Gerngross,et al. Enzyme-catalyzed synthesis of poly[(R)-(-)-3-hydroxybutyrate]: formation of macroscopic granules in vitro. , 1995, Proceedings of the National Academy of Sciences of the United States of America.
[81] A. Steinbüchel,et al. Diversity of bacterial polyhydroxyalkanoic acids , 1995 .
[82] A. Steinbüchel,et al. Identification of the region of a 14-kilodalton protein of Rhodococcus ruber that is responsible for the binding of this phasin to polyhydroxyalkanoic acid granules , 1995, Journal of bacteriology.
[83] A. Steinbüchel,et al. Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus , 1995, Journal of bacteriology.
[84] W. Pangborn,et al. Structure of uncomplexed and linoleate-bound Candida cylindracea cholesterol esterase. , 1995, Structure.
[85] Y. Poirier,et al. Synthesis of high-molecular-weight poly([R]-(-)-3-hydroxybutyrate) in transgenic Arabidopsis thaliana plant cells. , 1995, International journal of biological macromolecules.
[86] A. Steinbüchel,et al. Purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules. , 1994, European journal of biochemistry.
[87] U Derewenda,et al. Structure of a myristoyl-ACP-specific thioesterase from Vibrio harveyi. , 1994, Biochemistry.
[88] A. Sinskey,et al. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. , 1994, Biochemistry.
[89] M. Schembri,et al. Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located. , 1994, FEMS microbiology letters.
[90] B. Rost,et al. Combining evolutionary information and neural networks to predict protein secondary structure , 1994, Proteins.
[91] A. Sinskey,et al. Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules , 1993, Journal of bacteriology.
[92] B. Rost,et al. Prediction of protein secondary structure at better than 70% accuracy. , 1993, Journal of molecular biology.
[93] D. Seebach,et al. POLY(HYDROXYFETTSAUREESTER), EINE FUNFTE KLASSE VON PHYSIOLOGISCH BEDEUTSAMEN ORGANISCHEN BIOPOLYMEREN ? , 1993 .
[94] Michael J. McInerney,et al. Synthesis and function of polyhydroxyalkanoates in anaerobic syntrophic bacteria , 1992 .
[95] Y. Doi,et al. Synthesis and degradation of polyhydroxyalkanoates in Alcaligenes eutrophus , 1992 .
[96] A. Steinbüchel,et al. Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1. , 1992, European journal of biochemistry.
[97] A. Steinbüchel,et al. Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D. , 1992, European journal of biochemistry.
[98] Joel L. Sussman,et al. The α/β hydrolase fold , 1992 .
[99] A. Steinbüchel,et al. Physiology and molecular genetics of poly(β‐hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus , 1991, Molecular microbiology.
[100] P. Terpstra,et al. Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA. , 1991, The Journal of biological chemistry.
[101] A. Steinbüchel,et al. Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon: identification of the N terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter , 1991, Journal of bacteriology.
[102] A. Anderson,et al. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. , 1990, Microbiological reviews.
[103] D. Ollis,et al. Refined structure of dienelactone hydrolase at 1.8 A. , 1990, Journal of molecular biology.
[104] A. Sinskey,et al. Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). , 1989, The Journal of biological chemistry.
[105] G. W. Haywood,et al. The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus , 1989 .
[106] A. Steinbüchel,et al. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli , 1988, Journal of bacteriology.
[107] S. Slater,et al. Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway , 1988, Journal of bacteriology.
[108] R. Reusch,et al. Putative structure and functions of a poly-beta-hydroxybutyrate/calcium polyphosphate channel in bacterial plasma membranes. , 1988, Proceedings of the National Academy of Sciences of the United States of America.
[109] R. Doolittle,et al. A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.
[110] J. Merrick,et al. Metabolism of Poly-β-Hydroxybutyrate: Effect of Mild Alkaline Extraction on Native Poly-β-Hydroxybutyrate Granules , 1971, Journal of bacteriology.
[111] J. Merrick,et al. Metabolism of poly-beta-hydroxybutyrate. I. Purification, composition, and properties of native poly-beta-hydroxybutyrate granules from Bacillus megaterium. , 1968, Biochemistry.