Neutrophil lysosomal enzyme release and complement activation during cardiopulmonary bypass.

Complement activation and neutrophil degranulation were concomitantly studied during uncomplicated cardiopulmonary bypass (CPB). Plasma concentrations of complement factor C4, complement split product C3d, the neutrophil lysosomal enzyme elastase complexed with alpha 1-proteinase inhibitor (PI) and fibronectin were measured in 12 patients, C3d and elastase/PI increased significantly during CPB (volume-corrected results). The C3d rise was almost linear, whereas elastase/PI showed exponential increase. Mean elastase/PI and mean C3d concentrations at different times during CPB covaried closely. The study showed that during CPB neutrophil lysosomal enzyme release is intimately related to complement activation, although activation of the two systems may be caused by a common third activator within the extracorporeal circuit.

[1]  W. Watson,et al.  Albumin standards and the measurement of serum albumin with bromcresol green. , 1997, Clinica chimica acta; international journal of clinical chemistry.

[2]  D. Vergani,et al.  Pathways to complement activation during cardiopulmonary bypass. , 1984 .

[3]  A. Nielsen,et al.  Leukopenia and Release of Granulocyte Elastase: Interlinked Membrane-Dependent Events during Hemodialysis , 1984 .

[4]  W. Hörl,et al.  Release of granulocyte proteinases during hemodialysis. , 1983, American journal of nephrology.

[5]  I. Brandslund,et al.  Complement activation in shock associated with a surgically provoked bacteriaemia. , 1983, Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology.

[6]  F. Zaimi,et al.  Perioperative changes in complement associated with cardiopulmonary bypass. , 1982, British journal of anaesthesia.

[7]  N. Matthews,et al.  Cardiopulmonary bypass and complement activation , 1982, Anaesthesia.

[8]  H. Jacob,et al.  Complement-induced granulocyte aggregation in vivo. , 1981, The American journal of pathology.

[9]  I. Brandslund,et al.  Double-decker rocket immunoelectrophoresis for direct quantitation of complement C3 split products with C3d specificities in plasma. , 1981, Journal of immunological methods.

[10]  D. Stroncek,et al.  Complement activation and neutropenia occurring during cardiopulmonary bypass. , 1981, The Journal of thoracic and cardiovascular surgery.

[11]  E. Blackstone,et al.  Complement activation during cardiopulmonary bypass: evidence for generation of C3a and C5a anaphylatoxins. , 1981, The New England journal of medicine.

[12]  E. Jaffe,et al.  Plasma fibronectin (opsonic glycoprotein): its synthesis by vascular endothelial cells and role in cardiopulmonary integrity after trauma as related to reticuloendothelial function. , 1980, The American journal of medicine.

[13]  T. Hugli,et al.  Demonstration of specific C5a receptor on intact human polymorphonuclear leukocytes. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[14]  E. Welman,et al.  Lysosomal enzyme release during cardiopulmonary bypass , 1977, Anaesthesia.

[15]  A. Dalmasso,et al.  Hemodialysis leukopenia. Pulmonary vascular leukostasis resulting from complement activation by dialyzer cellophane membranes. , 1977, The Journal of clinical investigation.

[16]  R S Kronenberg,et al.  Complement and leukocyte-mediated pulmonary dysfunction in hemodialysis. , 1977, The New England journal of medicine.

[17]  K. Ohlsson,et al.  The disappearance of enzyme-inhibitor complexes from the circulation of man. , 1976, Clinical science and molecular medicine.

[18]  K. Adams,et al.  Erythrocyte filterability and lysosomal enzymes in patients requiring cardiopulmonary bypass. , 1975, Surgery.

[19]  K. Ohlsson,et al.  Neutral proteases of human granulocytes. III. Interaction between human granulocyte elastase and plasma protease inhibitors. , 1974, Scandinavian journal of clinical and laboratory investigation.

[20]  S. Neumann,et al.  “PMN-Elastase Assay”: Enzyme Immunoassay for Human Polymorphonuclear Elastase Complexed with α1-Proteinase Inhibitor , 1984, Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie.

[21]  I. Brandslund,et al.  Activation of the complement system and accumulation of hemoglobin-haptoglobin complexes in plasma during an adverse reaction to penicillin treatment. , 1983, International archives of allergy and applied immunology.

[22]  P. Venge,et al.  Neutrophil and eosinophil degranulation during hemodialysis are mediated by the dialysis membrane. , 1982, Nephron.

[23]  W. Massion,et al.  Proteolytic enzyme levels during cardiopulmonary bypass. , 1982, Advances in shock research.

[24]  P. Venge,et al.  Hemodialysis-induced increase in serum lactoferrin and serum eosinophil cationic protein as signs of local neutrophil and eosinophil degranulation. , 1981, Nephron.

[25]  Haslam Pl,et al.  Complement activation during cardiopulmonary bypass , 1980 .

[26]  R. Macgregor.  Granulocyte Adherence Changes Induced by Hemodialysis, Endotoxin, Epinephrine, and Glucocorticoids , 1977 .