论文信息 - Measurement of protein by spectrophotometry at 205 nm.

Measurement of protein by spectrophotometry at 205 nm.

Abstract A method is described for the measurement of protein concentration by using the peptide bond absorption at 205 nm. ϵ205 is estimated, allowing for the absorption due to Trp and Tyr residues, by measuring the absorbance at 280 nm as well as at 205 nm. The estimated ϵ205 is compared with the actual ϵ205 for a number of proteins, the mean error being less than 2%. This is about three times better than using an average ϵ2051 mg/ml of 31 and approaches the range of experimental error inherent in any method of protein estimation.

R K Scopes | R. Scopes

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