论文信息 - Detecting behaviorally relevant changes in extracellular dopamine with microdialysis

Detecting behaviorally relevant changes in extracellular dopamine with microdialysis

A method is described for monitoring extracellular levels of striatal dopamine in the rat during behavior. The extracellular fluid is sampled using a microdialysis probe modified for use in behaving animals. Dopamine concentration in the perfusate is determined every 5 min using an automated smallbore chromatographic system with electrochemical detection. The system is capable of detecting behaviorally related changes of 5 nM, in extracellular dopamine.

[1] L. Pellegrino,et al. stereotaxic atlas of the rat brain , 1967 .

[2] J. B. Justice,et al. Sampling considerations for on-line microbore liquid chromatography of brain dialysate. , 1986, Analytical chemistry.

[3] T. Ljungberg,et al. Intracerebral dialysis coupled to a novel activity box — a method to monitor dopamine release during behaviour , 1986, Pharmacology Biochemistry and Behavior.

[4] J. B. Justice,et al. Rapid sampling and determination of extracellular dopamine in vivo. , 1987, Analytical chemistry.

[5] J. B. Justice,et al. Neuroleptics increase striatal catecholamine metabolites but not ascorbic acid in dialyzed perfusate , 1984, Brain Research.

[6] U. Ungerstedt,et al. Effect of neuroleptic drugs on striatal dopamine release and metabolism in the awake rat studied by intracerebral dialysis. , 1984, European journal of pharmacology.